FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059674429> ?p ?o ?g. }

• W2059674429 endingPage "2481" @default.
• W2059674429 startingPage "2471" @default.
• W2059674429 abstract "Objective To clone and characterize human anti-Ro/SSA autoantibodies from a patient with primary Sjögren's syndrome (pSS). Methods Monoclonal antibodies (mAb) were raised from the peripheral blood of a patient with pSS using Epstein-Barr virus transformation and a hybridoma technique. Specificity was determined using cell extracts, recombinant Ro 52-kd, Ro 60-kd, and La proteins as well as Ro 52-kd peptides in enzyme-linked immunosorbent assay (ELISA) and Western blot. The immunofluorescence pattern was analyzed using cultured human and mouse cell lines. Complementary DNA was amplified by polymerase chain reaction, and Ig variable (V)–region genes were directly sequenced. Results Two human anti–Ro 52-kd mAb of IgM isotype, denoted SG1 and SG3, were cloned from the peripheral blood of a patient with pSS. The 2 mAb reacted with the Ro 52-kd antigen in cell extracts of human cell lines and mouse cell lines, and with purified human recombinant Ro 52-kd protein in ELISA and Western blot. SG1 reacted specifically with 1 peptide, amino acids 136–156, of the Ro 52-kd protein, and SG3 was mapped to react with a recombinant fragment representing amino acids 136–292. Immunofluorescence studies revealed cytoplasmic staining with both mAb. Both were encoded by VH3-family genes. SG1 was highly homologous to the DP-77 germ-line gene, with 2 replacement mutations and 1 silent. It utilized the DPL-11 germ-line gene from the Vλ2-family gene, with 1 silent mutation. SG3 was 100% homologous to the DP-47 germ-line gene, combined with a Vκ1-family gene that was 100% homologous to the A30 germ-line gene. Conclusion Two human mAb were demonstrated to be specific for the Ro 52-kd protein and to be directed against 2 different epitopes, 1 linear and 1 conformation-dependent, within a region previously described to be immunodominant. Somatic hypermutation appeared to be of minor importance in generating these 2 specificities." @default.
• W2059674429 created "2016-06-24" @default.
• W2059674429 creator A5000560776 @default.
• W2059674429 creator A5015208014 @default.
• W2059674429 creator A5037614272 @default.
• W2059674429 creator A5043056600 @default.
• W2059674429 creator A5054840940 @default.
• W2059674429 creator A5056189353 @default.
• W2059674429 creator A5070764431 @default.
• W2059674429 date "1999-11-01" @default.
• W2059674429 modified "2023-10-01" @default.
• W2059674429 title "Immunoglobulin variable genes and epitope recognition of human monoclonal anti-Ro 52-kd in primary Sj�gren's syndrome" @default.
• W2059674429 cites W1550034438 @default.
• W2059674429 cites W1576432283 @default.
• W2059674429 cites W174502258 @default.
• W2059674429 cites W1965052029 @default.
• W2059674429 cites W1969108300 @default.
• W2059674429 cites W1971181746 @default.
• W2059674429 cites W1973141899 @default.
• W2059674429 cites W1973449609 @default.
• W2059674429 cites W1976411430 @default.
• W2059674429 cites W1980614922 @default.
• W2059674429 cites W1982459140 @default.
• W2059674429 cites W1982764945 @default.
• W2059674429 cites W1983438002 @default.
• W2059674429 cites W1996319171 @default.
• W2059674429 cites W2000612383 @default.
• W2059674429 cites W2004036440 @default.
• W2059674429 cites W2004083487 @default.
• W2059674429 cites W2005353719 @default.
• W2059674429 cites W2005393972 @default.
• W2059674429 cites W2006964904 @default.
• W2059674429 cites W2010521626 @default.
• W2059674429 cites W2013594905 @default.
• W2059674429 cites W2017344650 @default.
• W2059674429 cites W2020897056 @default.
• W2059674429 cites W2021991795 @default.
• W2059674429 cites W2024716566 @default.
• W2059674429 cites W2030955172 @default.
• W2059674429 cites W2033011464 @default.
• W2059674429 cites W2035158781 @default.
• W2059674429 cites W2036178205 @default.
• W2059674429 cites W2036796300 @default.
• W2059674429 cites W2044070430 @default.
• W2059674429 cites W2046572926 @default.
• W2059674429 cites W2046800939 @default.
• W2059674429 cites W2049667382 @default.
• W2059674429 cites W2056254159 @default.
• W2059674429 cites W2072946918 @default.
• W2059674429 cites W2073579100 @default.
• W2059674429 cites W2078157295 @default.
• W2059674429 cites W2079132996 @default.
• W2059674429 cites W2079559236 @default.
• W2059674429 cites W2081338850 @default.
• W2059674429 cites W2090603099 @default.
• W2059674429 cites W2095212548 @default.
• W2059674429 cites W2101345672 @default.
• W2059674429 cites W2104505982 @default.
• W2059674429 cites W2112084656 @default.
• W2059674429 cites W2114148064 @default.
• W2059674429 cites W2120773157 @default.
• W2059674429 cites W2125354087 @default.
• W2059674429 cites W2132724017 @default.
• W2059674429 cites W2141216907 @default.
• W2059674429 cites W2160933629 @default.
• W2059674429 cites W2344266977 @default.
• W2059674429 cites W4245862679 @default.
• W2059674429 cites W4252780753 @default.
• W2059674429 doi "https://doi.org/10.1002/1529-0131(199911)42:11<2471::aid-anr26>3.0.co;2-i" @default.
• W2059674429 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10555043" @default.
• W2059674429 hasPublicationYear "1999" @default.
• W2059674429 type Work @default.
• W2059674429 sameAs 2059674429 @default.
• W2059674429 citedByCount "40" @default.
• W2059674429 countsByYear W20596744292012 @default.
• W2059674429 countsByYear W20596744292013 @default.
• W2059674429 countsByYear W20596744292014 @default.
• W2059674429 countsByYear W20596744292017 @default.
• W2059674429 countsByYear W20596744292018 @default.
• W2059674429 countsByYear W20596744292019 @default.
• W2059674429 countsByYear W20596744292021 @default.
• W2059674429 crossrefType "journal-article" @default.
• W2059674429 hasAuthorship W2059674429A5000560776 @default.
• W2059674429 hasAuthorship W2059674429A5015208014 @default.
• W2059674429 hasAuthorship W2059674429A5037614272 @default.
• W2059674429 hasAuthorship W2059674429A5043056600 @default.
• W2059674429 hasAuthorship W2059674429A5054840940 @default.
• W2059674429 hasAuthorship W2059674429A5056189353 @default.
• W2059674429 hasAuthorship W2059674429A5070764431 @default.
• W2059674429 hasConcept C104317684 @default.
• W2059674429 hasConcept C153911025 @default.
• W2059674429 hasConcept C159047783 @default.
• W2059674429 hasConcept C159654299 @default.
• W2059674429 hasConcept C195616568 @default.
• W2059674429 hasConcept C203014093 @default.
• W2059674429 hasConcept C2776415932 @default.
• W2059674429 hasConcept C2777991845 @default.
• W2059674429 hasConcept C2778236600 @default.

• next