FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059684607> ?p ?o ?g. }

• W2059684607 endingPage "331" @default.
• W2059684607 startingPage "325" @default.
• W2059684607 abstract "Background Surface enhanced laser desorption and ionization-time-of-flight (SELDI-TOF) is an evolving proteomic technology for improving biomarker discovery that allows for rapid and sensitive analysis of complex protein mixtures generated from body fluids, cells, and/or tissues. SELDI—based profiling identifies unique, differentially expressed proteins relating to specific cancer-related disease states. We utilized SELDI-TOF following pre-processing with molecular separation and chemical fractionation of cell membrane extracts from three Dunning rat prostate cancer cell lines of varying metastatic potential to search novel proteins that are differentially expressed. Methods Dunning rat cell sublines of variable (%) metastatic potential; G (0%), AT-1 (20%), and Mat-Ly-Lu (100%) were cultured in two different laboratories. Cell lysis was performed in a homogenation buffer (320 mM sucrose/50 mM Tris/0.5 mM PSMF) using Dounce homogenation. After centrifugation, the membrane pellet was washed 2× and then solublized in 2% CHAPS/8 M urea. This sample was further processed using positive pressure molecular ultrafiltration at 30 kDa or precipitation with 50% ammonium sulfate. Next, each sample was applied to an IMAC3-Ni ProteinChip (Ciphergen Biosystems, Freemont, CA) and analyzed using Ciphergen's Protein Biology System with protein peak analysis software. Results SELDI-TOF analysis differentiated the three Dunning rat cell sublines based upon protein concentration normalized profiles between 5,000 and 20,000 Da. The preparations from the three cells lines showed clear differences when the extracts from the metastatic sublines (AT-1 and MLL) were compared to the benign subline (G) for proteins with molecular weights of 9 kDa (decrease), 12 kDa (significant decrease), 14 kDa (decrease), and 17 kDa (significant gain). After pre-processing extracts with ammonium sulfate and molecular ultrafiltration, the molecular profile changes from one subline to the next became more apparent. Our results were reproducible using multiple runs including from Dunning cells cultured in a separate laboratory, and using different lots of SELDI ProteinChips. Conclusions The application of SELDI-TOF to a series of Dunning rat prostate cancer cell lines illustrated apparent changes in protein profiles among the three cell lines with known differences in metastatic biologic activity. SELDI-TOF identified four reproducible changes in protein expression in the AT1 and MLL metastatic cell sublines. Three of the expression changes were manifested as decreases, but one protein (17 kDa) was over-expressed in the AT1 and MLL cell lines. Emphasis will be placed on the isolation, purification, and characterization of the 17 kDa over-expressed protein and its potential role in PCa metastasis. © 2004 Wiley-Liss, Inc." @default.
• W2059684607 created "2016-06-24" @default.
• W2059684607 creator A5018471465 @default.
• W2059684607 creator A5021033341 @default.
• W2059684607 creator A5033793895 @default.
• W2059684607 creator A5036160440 @default.
• W2059684607 creator A5040294874 @default.
• W2059684607 creator A5063449536 @default.
• W2059684607 creator A5066312474 @default.
• W2059684607 creator A5073431093 @default.
• W2059684607 date "2004-09-01" @default.
• W2059684607 modified "2023-10-17" @default.
• W2059684607 title "Proteomic analysis of dunning prostate cancer cell lines with variable metastatic potential using SELDI-TOF" @default.
• W2059684607 cites W122509891 @default.
• W2059684607 cites W1994326152 @default.
• W2059684607 cites W2000305974 @default.
• W2059684607 cites W2014399915 @default.
• W2059684607 cites W2018840890 @default.
• W2059684607 cites W2019781881 @default.
• W2059684607 cites W2027913086 @default.
• W2059684607 cites W2029113441 @default.
• W2059684607 cites W2031992506 @default.
• W2059684607 cites W2048258947 @default.
• W2059684607 cites W2051830951 @default.
• W2059684607 cites W2057150473 @default.
• W2059684607 cites W2057218203 @default.
• W2059684607 cites W2061383965 @default.
• W2059684607 cites W2068746221 @default.
• W2059684607 cites W2071879495 @default.
• W2059684607 cites W2071914829 @default.
• W2059684607 cites W2075800153 @default.
• W2059684607 cites W2086878322 @default.
• W2059684607 cites W2091949263 @default.
• W2059684607 cites W2102807020 @default.
• W2059684607 cites W2106246854 @default.
• W2059684607 cites W2109046819 @default.
• W2059684607 cites W2117098825 @default.
• W2059684607 cites W2122765364 @default.
• W2059684607 cites W2129028825 @default.
• W2059684607 cites W2160658234 @default.
• W2059684607 cites W2161768644 @default.
• W2059684607 cites W2418620952 @default.
• W2059684607 doi "https://doi.org/10.1002/pros.20066" @default.
• W2059684607 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15264244" @default.
• W2059684607 hasPublicationYear "2004" @default.
• W2059684607 type Work @default.
• W2059684607 sameAs 2059684607 @default.
• W2059684607 citedByCount "39" @default.
• W2059684607 countsByYear W20596846072012 @default.
• W2059684607 countsByYear W20596846072014 @default.
• W2059684607 countsByYear W20596846072015 @default.
• W2059684607 countsByYear W20596846072016 @default.
• W2059684607 crossrefType "journal-article" @default.
• W2059684607 hasAuthorship W2059684607A5018471465 @default.
• W2059684607 hasAuthorship W2059684607A5021033341 @default.
• W2059684607 hasAuthorship W2059684607A5033793895 @default.
• W2059684607 hasAuthorship W2059684607A5036160440 @default.
• W2059684607 hasAuthorship W2059684607A5040294874 @default.
• W2059684607 hasAuthorship W2059684607A5063449536 @default.
• W2059684607 hasAuthorship W2059684607A5066312474 @default.
• W2059684607 hasAuthorship W2059684607A5073431093 @default.
• W2059684607 hasConcept C104317684 @default.
• W2059684607 hasConcept C104397665 @default.
• W2059684607 hasConcept C121608353 @default.
• W2059684607 hasConcept C153911025 @default.
• W2059684607 hasConcept C185592680 @default.
• W2059684607 hasConcept C190431759 @default.
• W2059684607 hasConcept C2778336440 @default.
• W2059684607 hasConcept C2780192828 @default.
• W2059684607 hasConcept C41625074 @default.
• W2059684607 hasConcept C46111723 @default.
• W2059684607 hasConcept C54355233 @default.
• W2059684607 hasConcept C55493867 @default.
• W2059684607 hasConcept C57409179 @default.
• W2059684607 hasConcept C7623868 @default.
• W2059684607 hasConcept C81885089 @default.
• W2059684607 hasConcept C86803240 @default.
• W2059684607 hasConceptScore W2059684607C104317684 @default.
• W2059684607 hasConceptScore W2059684607C104397665 @default.
• W2059684607 hasConceptScore W2059684607C121608353 @default.
• W2059684607 hasConceptScore W2059684607C153911025 @default.
• W2059684607 hasConceptScore W2059684607C185592680 @default.
• W2059684607 hasConceptScore W2059684607C190431759 @default.
• W2059684607 hasConceptScore W2059684607C2778336440 @default.
• W2059684607 hasConceptScore W2059684607C2780192828 @default.
• W2059684607 hasConceptScore W2059684607C41625074 @default.
• W2059684607 hasConceptScore W2059684607C46111723 @default.
• W2059684607 hasConceptScore W2059684607C54355233 @default.
• W2059684607 hasConceptScore W2059684607C55493867 @default.
• W2059684607 hasConceptScore W2059684607C57409179 @default.
• W2059684607 hasConceptScore W2059684607C7623868 @default.
• W2059684607 hasConceptScore W2059684607C81885089 @default.
• W2059684607 hasConceptScore W2059684607C86803240 @default.
• W2059684607 hasIssue "4" @default.
• W2059684607 hasLocation W20596846071 @default.
• W2059684607 hasLocation W20596846072 @default.
• W2059684607 hasOpenAccess W2059684607 @default.
• W2059684607 hasPrimaryLocation W20596846071 @default.

• next