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- W2059688003 abstract "1. The effect of pH on the buoyant density of the complexes of Ag+ with DNA has been studied using 3H-labeled human DNA and several bacterial DNAs to determine the conditions necessary for the maximum resolution of compositional heterogeneity. In neutral Cs2SO4 density gradients, Ag+ complexes with (G · C)-rich components are always denser than those with (A · T)-rich components, since (G · C)rich DNAs have a larger affinity for Ag+ than (A · T)-rich DNAs and their complexes are denser than (A · T)-rich complexes. In alkaline (pH > 9) Cs2SO4 gradients, the buoyant density of the Ag+ · DNA complex is not a simple function of base composition. The Ag+ affinity of (A · T)-rich DNA is larger than that of (G · C)-rich DNA but the density of a (G · C)-rich complex is larger. Thus the ordering of the buoyant density changes depends on the amount of added Ag+. 2. The problem of resolving the density heterogeneity within a tracer DNA, and minor components of DNA, is explored and useful fractionation techniques are developed." @default.
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- W2059688003 date "1976-04-01" @default.
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- W2059688003 title "The effect of silver ion binding and pH on the buoyant density of DNA and its use in fractionating heterogeneous DNA" @default.
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- W2059688003 doi "https://doi.org/10.1016/0005-2787(76)90009-5" @default.
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