Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059696845> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2059696845 endingPage "282" @default.
- W2059696845 startingPage "270" @default.
- W2059696845 abstract "The pnd system of plasmid R483 mediates plasmid stabilization by killing of plasmid-free cells. The pnd mRNA is very stable and can be translated into PndA protein, a cell toxin which kills the cells from within by damaging the cell membrane. Translation of the pnd mRNA is inhibited by the PndB antisense, a small labile RNA of 63 nt. The rapid decay of the PndB antidote leads to onset of PndA synthesis in plasmid-free segregants or after addition of rifampicin. Surprisingly however, the full-length pnd mRNA was found to be translationally inactive whereas a 3′-end truncated version of it was found to be active. We have therefore suggested previously, that the 3′-end of the full-length pnd mRNA encodes a fold-back inhibitory sequence (fbi), which prevents its translation. Here we present an analysis of the metabolism of the pnd mRNAs. A mutational analysis shows that single point mutations in the fbi motif results in more rapid truncation. The fbi mutations could not be complemented by second-site mutations in either of the pndA or pndC Shine-Dalgarno (SD) elements. Surprisingly, mutations in the pndC SD element also lead to a more rapid truncation. The effect of these latter mutations was, however, complemented by mutations in a proposed anti-SD element upstream of the pndC SD. Mutations in the anti-SD element were lethal. These results show, that the pnd mRNA contains two negative control elements, one located in its very 3′-end (fbi), and one located just upstream of the pndC SD region (the anti-SD element). These observations add to the complexity of the induction scheme previously proposed to explain activation of pndA expression in plasmid-free cells: In addition to its negative effect of translation, the fbi structure also maintains a reduced processing rate in the 3′-end of the mRNA. This permits the accumulation of a reservoir of pnd mRNA, which can be activated by 3′-end processing in plasmid-free cells. The anti-SD may prevent translation of the pnd mRNA during transcription, thus preventing detrimental synthesis of toxin." @default.
- W2059696845 created "2016-06-24" @default.
- W2059696845 creator A5070618817 @default.
- W2059696845 creator A5077807435 @default.
- W2059696845 date "1995-01-01" @default.
- W2059696845 modified "2023-10-16" @default.
- W2059696845 title "Mechanism of post-segregational killing by hok-homologue pnd of plasmid R483: Two translational control elements in the pnd mRNA" @default.
- W2059696845 cites W112158789 @default.
- W2059696845 cites W1500916922 @default.
- W2059696845 cites W1511335270 @default.
- W2059696845 cites W1585145369 @default.
- W2059696845 cites W1588276940 @default.
- W2059696845 cites W1605290272 @default.
- W2059696845 cites W166920987 @default.
- W2059696845 cites W17016481 @default.
- W2059696845 cites W1936065549 @default.
- W2059696845 cites W1970852096 @default.
- W2059696845 cites W1977238968 @default.
- W2059696845 cites W1984124809 @default.
- W2059696845 cites W1996039015 @default.
- W2059696845 cites W2009301315 @default.
- W2059696845 cites W2014776977 @default.
- W2059696845 cites W2016245855 @default.
- W2059696845 cites W2019616994 @default.
- W2059696845 cites W2022456092 @default.
- W2059696845 cites W2024468809 @default.
- W2059696845 cites W2069477714 @default.
- W2059696845 cites W2070863882 @default.
- W2059696845 cites W2116950977 @default.
- W2059696845 cites W2127372563 @default.
- W2059696845 cites W2127827249 @default.
- W2059696845 cites W2129262822 @default.
- W2059696845 cites W2131423362 @default.
- W2059696845 cites W2146495422 @default.
- W2059696845 cites W2166489116 @default.
- W2059696845 cites W2197678796 @default.
- W2059696845 cites W2213221235 @default.
- W2059696845 cites W2916217134 @default.
- W2059696845 cites W348060877 @default.
- W2059696845 doi "https://doi.org/10.1006/jmbi.1995.0296" @default.
- W2059696845 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7783193" @default.
- W2059696845 hasPublicationYear "1995" @default.
- W2059696845 type Work @default.
- W2059696845 sameAs 2059696845 @default.
- W2059696845 citedByCount "14" @default.
- W2059696845 countsByYear W20596968452012 @default.
- W2059696845 countsByYear W20596968452014 @default.
- W2059696845 countsByYear W20596968452017 @default.
- W2059696845 countsByYear W20596968452020 @default.
- W2059696845 countsByYear W20596968452021 @default.
- W2059696845 crossrefType "journal-article" @default.
- W2059696845 hasAuthorship W2059696845A5070618817 @default.
- W2059696845 hasAuthorship W2059696845A5077807435 @default.
- W2059696845 hasConcept C104317684 @default.
- W2059696845 hasConcept C105580179 @default.
- W2059696845 hasConcept C149364088 @default.
- W2059696845 hasConcept C153911025 @default.
- W2059696845 hasConcept C176944494 @default.
- W2059696845 hasConcept C22744801 @default.
- W2059696845 hasConcept C3675279 @default.
- W2059696845 hasConcept C501734568 @default.
- W2059696845 hasConcept C54355233 @default.
- W2059696845 hasConcept C67705224 @default.
- W2059696845 hasConcept C86803240 @default.
- W2059696845 hasConcept C95444343 @default.
- W2059696845 hasConceptScore W2059696845C104317684 @default.
- W2059696845 hasConceptScore W2059696845C105580179 @default.
- W2059696845 hasConceptScore W2059696845C149364088 @default.
- W2059696845 hasConceptScore W2059696845C153911025 @default.
- W2059696845 hasConceptScore W2059696845C176944494 @default.
- W2059696845 hasConceptScore W2059696845C22744801 @default.
- W2059696845 hasConceptScore W2059696845C3675279 @default.
- W2059696845 hasConceptScore W2059696845C501734568 @default.
- W2059696845 hasConceptScore W2059696845C54355233 @default.
- W2059696845 hasConceptScore W2059696845C67705224 @default.
- W2059696845 hasConceptScore W2059696845C86803240 @default.
- W2059696845 hasConceptScore W2059696845C95444343 @default.
- W2059696845 hasIssue "2" @default.
- W2059696845 hasLocation W20596968451 @default.
- W2059696845 hasLocation W20596968452 @default.
- W2059696845 hasOpenAccess W2059696845 @default.
- W2059696845 hasPrimaryLocation W20596968451 @default.
- W2059696845 hasRelatedWork W127805044 @default.
- W2059696845 hasRelatedWork W1571771606 @default.
- W2059696845 hasRelatedWork W1982581831 @default.
- W2059696845 hasRelatedWork W2032977728 @default.
- W2059696845 hasRelatedWork W2046029527 @default.
- W2059696845 hasRelatedWork W2055435782 @default.
- W2059696845 hasRelatedWork W2064856200 @default.
- W2059696845 hasRelatedWork W2081714384 @default.
- W2059696845 hasRelatedWork W2399870116 @default.
- W2059696845 hasRelatedWork W4256246649 @default.
- W2059696845 hasVolume "249" @default.
- W2059696845 isParatext "false" @default.
- W2059696845 isRetracted "false" @default.
- W2059696845 magId "2059696845" @default.
- W2059696845 workType "article" @default.