FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059697503> ?p ?o ?g. }

• W2059697503 endingPage "25849" @default.
• W2059697503 startingPage "25845" @default.
• W2059697503 abstract "Liposomes recovered from the blood of liposome-treated CD1 mice were previously reported to have a complex protein profile associated with their membranes (Chonn, A., Semple, S. C., and Cullis, P. R.(1992) J. Biol. Chem. 267, 18759-18765). In this study, we have further characterized and identified the major proteins associated with very rapidly cleared large unilamellar vesicles. These liposomes contained phosphatidylcholine, cholesterol, and anionic phospholipids (phosphatidylserine, phosphatidic acid, or cardiolipin) that dramatically enhance the clearance rate of liposomes from the circulation. These anionic phospholipids are normally found exclusively in the interior of cells but become expressed when cells undergo apoptosis or programmed cell death, and thus, they are believed to be markers of cell senescence. Analysis of the proteins associated with these liposomes by SDS-polyacrylamide gel electrophoresis revealed that two of the major proteins associated with the liposome membranes are proteins with electrophoretic mobilities corresponding to Mr of 66,000 and 50,000-55,000. The 66-kDa protein was identified to be serum albumin by immunoblot analysis. Using various biochemical and immunological methods, we have identified the 50-55-kDa protein as the murine equivalent of human β2-glycoprotein I. β2-glycoprotein I has a strong affinity for phosphatidylserine, phosphatidic acid, and cardiolipin inasmuch as the levels of β2-glycoprotein I associated with these anionic liposomes approach or even exceed those of serum albumin, which is present in serum at a concentration 200-fold greater than β2-glycoprotein I. Further, we demonstrate that the amount of β2-glycoprotein I associated with liposomes, as quantitated by an enzyme-linked immunosorbent assay, is correlated with their clearance rates; moreover, the circulation residency time of cardiolipin-containing liposomes is extended in mice pretreated with anti-β2-glycoprotein I antibodies. These findings strongly suggest that β2-glycoprotein I plays a primary role in mediating the clearance of liposomes and, by extension, senescent cells and foreign particles. Liposomes recovered from the blood of liposome-treated CD1 mice were previously reported to have a complex protein profile associated with their membranes (Chonn, A., Semple, S. C., and Cullis, P. R.(1992) J. Biol. Chem. 267, 18759-18765). In this study, we have further characterized and identified the major proteins associated with very rapidly cleared large unilamellar vesicles. These liposomes contained phosphatidylcholine, cholesterol, and anionic phospholipids (phosphatidylserine, phosphatidic acid, or cardiolipin) that dramatically enhance the clearance rate of liposomes from the circulation. These anionic phospholipids are normally found exclusively in the interior of cells but become expressed when cells undergo apoptosis or programmed cell death, and thus, they are believed to be markers of cell senescence. Analysis of the proteins associated with these liposomes by SDS-polyacrylamide gel electrophoresis revealed that two of the major proteins associated with the liposome membranes are proteins with electrophoretic mobilities corresponding to Mr of 66,000 and 50,000-55,000. The 66-kDa protein was identified to be serum albumin by immunoblot analysis. Using various biochemical and immunological methods, we have identified the 50-55-kDa protein as the murine equivalent of human β2-glycoprotein I. β2-glycoprotein I has a strong affinity for phosphatidylserine, phosphatidic acid, and cardiolipin inasmuch as the levels of β2-glycoprotein I associated with these anionic liposomes approach or even exceed those of serum albumin, which is present in serum at a concentration 200-fold greater than β2-glycoprotein I. Further, we demonstrate that the amount of β2-glycoprotein I associated with liposomes, as quantitated by an enzyme-linked immunosorbent assay, is correlated with their clearance rates; moreover, the circulation residency time of cardiolipin-containing liposomes is extended in mice pretreated with anti-β2-glycoprotein I antibodies. These findings strongly suggest that β2-glycoprotein I plays a primary role in mediating the clearance of liposomes and, by extension, senescent cells and foreign particles." @default.
• W2059697503 created "2016-06-24" @default.
• W2059697503 creator A5016337965 @default.
• W2059697503 creator A5038339810 @default.
• W2059697503 creator A5050350074 @default.
• W2059697503 date "1995-10-01" @default.
• W2059697503 modified "2023-10-14" @default.
• W2059697503 title "β2-Glycoprotein I Is a Major Protein Associated with Very Rapidly Cleared Liposomes in Vivo, Suggesting a Significant Role in the Immune Clearance of “Non-self” Particles" @default.
• W2059697503 cites W1500801849 @default.
• W2059697503 cites W1527910795 @default.
• W2059697503 cites W1564122790 @default.
• W2059697503 cites W1597997684 @default.
• W2059697503 cites W1608305635 @default.
• W2059697503 cites W1940692702 @default.
• W2059697503 cites W1966527905 @default.
• W2059697503 cites W1969013901 @default.
• W2059697503 cites W1980126098 @default.
• W2059697503 cites W1988814356 @default.
• W2059697503 cites W1991505001 @default.
• W2059697503 cites W2000055469 @default.
• W2059697503 cites W2000886437 @default.
• W2059697503 cites W2007201040 @default.
• W2059697503 cites W2011577434 @default.
• W2059697503 cites W2013827296 @default.
• W2059697503 cites W2017628675 @default.
• W2059697503 cites W2020781643 @default.
• W2059697503 cites W2023052741 @default.
• W2059697503 cites W2024546396 @default.
• W2059697503 cites W2052245233 @default.
• W2059697503 cites W2059243929 @default.
• W2059697503 cites W2062447821 @default.
• W2059697503 cites W2080779467 @default.
• W2059697503 cites W2085092548 @default.
• W2059697503 cites W2091528120 @default.
• W2059697503 cites W2093870776 @default.
• W2059697503 cites W2101831374 @default.
• W2059697503 cites W2107203783 @default.
• W2059697503 cites W2113891001 @default.
• W2059697503 cites W2126662679 @default.
• W2059697503 cites W2138858884 @default.
• W2059697503 cites W2326622458 @default.
• W2059697503 cites W4240479615 @default.
• W2059697503 doi "https://doi.org/10.1074/jbc.270.43.25845" @default.
• W2059697503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7592769" @default.
• W2059697503 hasPublicationYear "1995" @default.
• W2059697503 type Work @default.
• W2059697503 sameAs 2059697503 @default.
• W2059697503 citedByCount "167" @default.
• W2059697503 countsByYear W20596975032012 @default.
• W2059697503 countsByYear W20596975032013 @default.
• W2059697503 countsByYear W20596975032014 @default.
• W2059697503 countsByYear W20596975032015 @default.
• W2059697503 countsByYear W20596975032016 @default.
• W2059697503 countsByYear W20596975032017 @default.
• W2059697503 countsByYear W20596975032018 @default.
• W2059697503 countsByYear W20596975032019 @default.
• W2059697503 countsByYear W20596975032020 @default.
• W2059697503 countsByYear W20596975032021 @default.
• W2059697503 countsByYear W20596975032022 @default.
• W2059697503 countsByYear W20596975032023 @default.
• W2059697503 crossrefType "journal-article" @default.
• W2059697503 hasAuthorship W2059697503A5016337965 @default.
• W2059697503 hasAuthorship W2059697503A5038339810 @default.
• W2059697503 hasAuthorship W2059697503A5050350074 @default.
• W2059697503 hasBestOaLocation W20596975031 @default.
• W2059697503 hasConcept C108625454 @default.
• W2059697503 hasConcept C130316041 @default.
• W2059697503 hasConcept C146543888 @default.
• W2059697503 hasConcept C165616093 @default.
• W2059697503 hasConcept C185154212 @default.
• W2059697503 hasConcept C185592680 @default.
• W2059697503 hasConcept C2776125364 @default.
• W2059697503 hasConcept C2776330855 @default.
• W2059697503 hasConcept C2776909261 @default.
• W2059697503 hasConcept C2778541144 @default.
• W2059697503 hasConcept C2778597219 @default.
• W2059697503 hasConcept C2778918659 @default.
• W2059697503 hasConcept C2779637612 @default.
• W2059697503 hasConcept C2781302388 @default.
• W2059697503 hasConcept C41625074 @default.
• W2059697503 hasConcept C55493867 @default.
• W2059697503 hasConcept C86803240 @default.
• W2059697503 hasConceptScore W2059697503C108625454 @default.
• W2059697503 hasConceptScore W2059697503C130316041 @default.
• W2059697503 hasConceptScore W2059697503C146543888 @default.
• W2059697503 hasConceptScore W2059697503C165616093 @default.
• W2059697503 hasConceptScore W2059697503C185154212 @default.
• W2059697503 hasConceptScore W2059697503C185592680 @default.
• W2059697503 hasConceptScore W2059697503C2776125364 @default.
• W2059697503 hasConceptScore W2059697503C2776330855 @default.
• W2059697503 hasConceptScore W2059697503C2776909261 @default.
• W2059697503 hasConceptScore W2059697503C2778541144 @default.
• W2059697503 hasConceptScore W2059697503C2778597219 @default.
• W2059697503 hasConceptScore W2059697503C2778918659 @default.
• W2059697503 hasConceptScore W2059697503C2779637612 @default.
• W2059697503 hasConceptScore W2059697503C2781302388 @default.
• W2059697503 hasConceptScore W2059697503C41625074 @default.
• W2059697503 hasConceptScore W2059697503C55493867 @default.

• next