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- W2059720166 abstract "The inclusion of urea has been found to eliminate adsorption of protein-sodium dodecyl sulphate (SDS) complexes to controlled pore glass. Using buffer containing 6 M urea, 0.5% SDS and glass with pore diameter 12.3 nm, it is possible to determine protein molecular weights in the range 3500-12,000. Results with glass of larger pore diameter (25.5 nm) are similar to those reported in the absence of urea in the molecular-weight range 12,000-140,000. Controlled pore glass chromatography also permits the study of the relative importance of conformation free of charge effects for those proteins which deviate from the normal calibration curve for SDS-polyacrylamide gels." @default.
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- W2059720166 title "Controlled pore glass chromatography of protein-sodium dodecyl sulphate complexes" @default.
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- W2059720166 doi "https://doi.org/10.1016/s0021-9673(00)99289-6" @default.
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