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- W2059762004 abstract "The emerging technique of three-dimensional (3D) printing provides a revolutionary way to fabricate objects with biologically realistic geometries. Previously we have performed optical and morphological characterization of basic 3D printed tissue-simulating phantoms and found them suitable for use in evaluating biophotonic imaging systems. In this study we assess the potential for printing phantoms with irregular, image-defined vascular networks that can be used to provide clinically-relevant insights into device performance. A previously acquired fundus camera image of the human retina was segmented, embedded into a 3D matrix, edited to incorporate the tubular shape of vessels and converted into a digital format suitable for printing. A polymer with biologically realistic optical properties was identified by spectrophotometer measurements of several commercially available samples. Phantoms were printed with the retinal vascular network reproduced as ~1.0 mm diameter channels at a range of depths up to ~3 mm. The morphology of the printed vessels was verified by volumetric imaging with μ-CT. Channels were filled with hemoglobin solutions at controlled oxygenation levels, and the phantoms were imaged by a near-infrared hyperspectral reflectance imaging system. The effect of vessel depth on hemoglobin saturation estimates was studied. Additionally, a phantom incorporating the vascular network at two depths was printed and filled with hemoglobin solution at two different saturation levels. Overall, results indicated that 3D printed phantoms are useful for assessing biophotonic system performance and have the potential to form the basis of clinically-relevant standardized test methods for assessment of medical imaging modalities." @default.
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- W2059762004 date "2015-03-06" @default.
- W2059762004 modified "2023-10-18" @default.
- W2059762004 title "3D printed biomimetic vascular phantoms for assessment of hyperspectral imaging systems" @default.
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- W2059762004 doi "https://doi.org/10.1117/12.2084720" @default.
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