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- W2059762010 abstract "For the analysis of mutation in human T-cell lymphocytes, it is crucial to determine the clonal relationship between isolated mutants, particularly if they harbour identical mutations. Here we report an efficient method to determine the clonal relationships between these cells. The method is based on the analysis of restriction fragment length polymorphisms of a polymerase chain reaction amplified, rearranged T-cell receptor γ-gene. As few as 600 cells are sufficient, regular agarose gels can be used for the separation of the restriction fragments, no radioactive label is required, and results can be obtained in 2 days." @default.
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- W2059762010 date "1993-07-01" @default.
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- W2059762010 title "A fast and simple method to determine the clonal relationship among human T-cell lymphocytes" @default.
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- W2059762010 doi "https://doi.org/10.1016/0027-5107(93)90218-5" @default.
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