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- W2059767183 abstract "Due to its spectral characteristics, the fluorochrome nonyl acridine orange (NAO) (λabs:489 nm, λem:525 nm), which is spontaneously incorporated by mitochondria with a high relative specificity, provides a new probe for the in situ study of these organelles by flow cytometry. In 15 min at 20°C, the dye at 4.75 × 10−6 M saturates the mitochondrial binding sites present in 1.5 × 106 cells. Unlike Rh 123, the fixation of the probe is not affected by the action of uncouplers and ionophores. Unlike acridine orange, its binding is not sensitive to nucleases. By studying the mitochondrial incorporation of the fluorochrome during the cell cycle of murine splenocytes, it was possible to show that the biogenesis of NAO-stained mitochondrial constituents mainly occurs during the G1 phase." @default.
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- W2059767183 date "1988-05-01" @default.
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- W2059767183 title "In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes" @default.
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- W2059767183 doi "https://doi.org/10.1002/cyto.990090304" @default.
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