FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059771211> ?p ?o ?g. }

• W2059771211 endingPage "1069" @default.
• W2059771211 startingPage "1061" @default.
• W2059771211 abstract "Cyclic-GMP-dependent protein kinase (PKG) is widely appreciated as having diverse roles in a variety of cell types. Many reports have indicated that PKG might regulate cell function by activating members of the mitogen-activated protein kinase (MAPK) family of signaling proteins. In this study, stimulation of HEK-293 cells with nitric oxide (NO) was found to induce a rapid accumulation of phosphorylated p38 MAPK. The involvement of PKG in this process was confirmed by cotransfection of a dominant negative PKG construct (G1αR-GFP), which was able to block cGMP-induced p38 MAPK activation. Transfection of cells to express dominant negative Rac1(T17N) was also able to dose-dependently block cGMP-stimulated activation of p38 MAPK, thus indicating the importance of this pathway downstream of PKG. GST-PDB affinity-precipitation experiments revealed that stimulation of HEK293 cells with either nitric oxide or 8-Br-cGMP resulted in a rapid and transient activation of Rac1 with similar kinetics to p38 MAPK phosphorylation. Moreover, using in vitro kinase assays it was found that cGMP also stimulated the activity of the Rac1 effector Pak1. The activation of both Rac1 and Pak1 by 8-Br-cGMP was completely abolished by transfection of the cells with G1αR-GFP. Expression of the Rac1(T17N) mutant inhibited PKG-dependent activation of PAK1 indicating that Rac1 functions upstream of PAK1 in this pathway. Immunofluorescence experiments demonstrated clear colocalization of PKG and Rac1 in membrane ruffles and dynamic membrane regions supporting a functional interaction. However, in vitro kinase assays demonstrated that Rac1 is not a substrate for PKG suggesting an indirect activation mechanism. Taken together these data demonstrate a novel PKG-dependent pathway by which the Rac1/Pak1 pathway is activated. Furthermore, we demonstrate that this pathway is central to the activation of p38 MAPK by PKG in these cells." @default.
• W2059771211 created "2016-06-24" @default.
• W2059771211 creator A5059867240 @default.
• W2059771211 creator A5066886337 @default.
• W2059771211 creator A5091494509 @default.
• W2059771211 date "2004-09-01" @default.
• W2059771211 modified "2023-10-05" @default.
• W2059771211 title "Activation of the small GTPase Rac1 by cGMP-dependent protein kinase" @default.
• W2059771211 cites W1526841021 @default.
• W2059771211 cites W1534699330 @default.
• W2059771211 cites W1555692789 @default.
• W2059771211 cites W1599507049 @default.
• W2059771211 cites W1902300865 @default.
• W2059771211 cites W1984387240 @default.
• W2059771211 cites W1985462906 @default.
• W2059771211 cites W1996374693 @default.
• W2059771211 cites W1998355348 @default.
• W2059771211 cites W2001148052 @default.
• W2059771211 cites W2005792688 @default.
• W2059771211 cites W2006335551 @default.
• W2059771211 cites W2016763579 @default.
• W2059771211 cites W2021047807 @default.
• W2059771211 cites W2029008901 @default.
• W2059771211 cites W2029242493 @default.
• W2059771211 cites W2034481155 @default.
• W2059771211 cites W2034580062 @default.
• W2059771211 cites W2047330178 @default.
• W2059771211 cites W2055181868 @default.
• W2059771211 cites W2064439294 @default.
• W2059771211 cites W2080573406 @default.
• W2059771211 cites W2090332465 @default.
• W2059771211 cites W2118408607 @default.
• W2059771211 cites W2121452261 @default.
• W2059771211 cites W2137377367 @default.
• W2059771211 cites W2145357604 @default.
• W2059771211 cites W2155868854 @default.
• W2059771211 cites W2171432125 @default.
• W2059771211 cites W29946730 @default.
• W2059771211 doi "https://doi.org/10.1016/j.cellsig.2004.03.002" @default.
• W2059771211 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15212766" @default.
• W2059771211 hasPublicationYear "2004" @default.
• W2059771211 type Work @default.
• W2059771211 sameAs 2059771211 @default.
• W2059771211 citedByCount "21" @default.
• W2059771211 countsByYear W20597712112012 @default.
• W2059771211 countsByYear W20597712112013 @default.
• W2059771211 countsByYear W20597712112014 @default.
• W2059771211 countsByYear W20597712112015 @default.
• W2059771211 countsByYear W20597712112016 @default.
• W2059771211 countsByYear W20597712112020 @default.
• W2059771211 crossrefType "journal-article" @default.
• W2059771211 hasAuthorship W2059771211A5059867240 @default.
• W2059771211 hasAuthorship W2059771211A5066886337 @default.
• W2059771211 hasAuthorship W2059771211A5091494509 @default.
• W2059771211 hasConcept C104317684 @default.
• W2059771211 hasConcept C153911025 @default.
• W2059771211 hasConcept C184235292 @default.
• W2059771211 hasConcept C185592680 @default.
• W2059771211 hasConcept C199217515 @default.
• W2059771211 hasConcept C24901178 @default.
• W2059771211 hasConcept C2779717556 @default.
• W2059771211 hasConcept C54009773 @default.
• W2059771211 hasConcept C55493867 @default.
• W2059771211 hasConcept C57074206 @default.
• W2059771211 hasConcept C62478195 @default.
• W2059771211 hasConcept C86803240 @default.
• W2059771211 hasConcept C90934575 @default.
• W2059771211 hasConcept C95444343 @default.
• W2059771211 hasConcept C97029542 @default.
• W2059771211 hasConceptScore W2059771211C104317684 @default.
• W2059771211 hasConceptScore W2059771211C153911025 @default.
• W2059771211 hasConceptScore W2059771211C184235292 @default.
• W2059771211 hasConceptScore W2059771211C185592680 @default.
• W2059771211 hasConceptScore W2059771211C199217515 @default.
• W2059771211 hasConceptScore W2059771211C24901178 @default.
• W2059771211 hasConceptScore W2059771211C2779717556 @default.
• W2059771211 hasConceptScore W2059771211C54009773 @default.
• W2059771211 hasConceptScore W2059771211C55493867 @default.
• W2059771211 hasConceptScore W2059771211C57074206 @default.
• W2059771211 hasConceptScore W2059771211C62478195 @default.
• W2059771211 hasConceptScore W2059771211C86803240 @default.
• W2059771211 hasConceptScore W2059771211C90934575 @default.
• W2059771211 hasConceptScore W2059771211C95444343 @default.
• W2059771211 hasConceptScore W2059771211C97029542 @default.
• W2059771211 hasIssue "9" @default.
• W2059771211 hasLocation W20597712111 @default.
• W2059771211 hasLocation W20597712112 @default.
• W2059771211 hasOpenAccess W2059771211 @default.
• W2059771211 hasPrimaryLocation W20597712111 @default.
• W2059771211 hasRelatedWork W1989455736 @default.
• W2059771211 hasRelatedWork W2000302322 @default.
• W2059771211 hasRelatedWork W2004726042 @default.
• W2059771211 hasRelatedWork W2066637872 @default.
• W2059771211 hasRelatedWork W2072243374 @default.
• W2059771211 hasRelatedWork W2095255069 @default.
• W2059771211 hasRelatedWork W2144611693 @default.
• W2059771211 hasRelatedWork W2350517500 @default.
• W2059771211 hasRelatedWork W3035328115 @default.

• next