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- W2059785315 abstract "ABSTRACT FlhF is a signal recognition particle-like protein present in monotrichous bacteria. The loss of FlhF in various bacteria results in decreased transcription of class II, III, or IV flagellar genes, leads to diminished or absent motility, and results in the assembly of flagella at nonpolar locations on the cell surface. In this work, we demonstrate that the loss of FlhF results in defective swimming and swarming motility of Pseudomonas aeruginosa . The FlhF protein localizes to the flagellar pole; in the absence of FlhF, flagellar assembly occurs but is no longer restricted to the pole. Δ flhF bacteria swim at lower velocities than wild-type bacteria in liquid media and can no longer swarm when assayed under standard swarming conditions (0.5% agar). However, Δ flhF bacteria regain swarming behavior when plated on 0.3% agar. Δ flhF organisms show decreased transcription and expression of flagellin (FliC) both in liquid media and on swarming plates compared to wild-type bacteria. However, changes in flagellin expression do not explain the different motility patterns observed for Δ flhF bacteria. Instead, the aberrant placement of flagella in Δ flhF bacteria may reduce their ability to move this rod-shaped organism effectively." @default.
- W2059785315 created "2016-06-24" @default.
- W2059785315 creator A5029651247 @default.
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- W2059785315 date "2006-10-01" @default.
- W2059785315 modified "2023-10-03" @default.
- W2059785315 title "FlhF Is Required for Swimming and Swarming in <i>Pseudomonas aeruginosa</i>" @default.
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- W2059785315 doi "https://doi.org/10.1128/jb.00790-06" @default.
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