FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059816522> ?p ?o ?g. }

• W2059816522 abstract "This study was designed to measure the survival and development of pronuclear stage bovine zygotes after microinjection with DNA and either culture in vitro or incubation in the sheep oviduct and to determine the percentage of embryos that retain exogenous DNA at the blastocyst stage. In vitro matured and fertilized oocytes were examined for pronuclear development 18 to 20 h after coincubation with sperm. An ovine somatotropin gene construct was microinjected into ova with visible pronuclei. Microinjected ova and unmanipulated ova from the same in vitro derived pool were cocultured with oviductal epithelial cells or incubated in the sheep oviduct and cocultured, respectively, for 7 d. Blastocysts were subjected to polymerase chain reaction analysis for detection of exogenous DNA. The percentage of cleaved embryos that formed blastocysts was similar for microinjected and unmanipulated cultured ova. The percentage of blastocysts recovered from the sheep oviduct that hatched was higher than for blastocysts that developed in coculture. Fourteen of 26 (54%) blastocysts that developed from microinjected ova were positive for the exogenous DNA by polymerase chain reaction. The implications are discussed for polymerase chain reaction detection of the exogenous gene construct in a relatively high proportion of microinjected blastocysts." @default.
• W2059816522 created "2016-06-24" @default.
• W2059816522 creator A5014699274 @default.
• W2059816522 creator A5017386242 @default.
• W2059816522 creator A5031061558 @default.
• W2059816522 creator A5045814298 @default.
• W2059816522 creator A5053694306 @default.
• W2059816522 creator A5057791131 @default.
• W2059816522 date "1993-11-01" @default.
• W2059816522 modified "2023-09-29" @default.
• W2059816522 title "Microinjection of Bovine Embryos with a Foreign Gene and Its Detection at the Blastocyst Stage" @default.
• W2059816522 cites W1833131266 @default.
• W2059816522 cites W1968458490 @default.
• W2059816522 cites W1976386694 @default.
• W2059816522 cites W1978224730 @default.
• W2059816522 cites W1983232505 @default.
• W2059816522 cites W1987834144 @default.
• W2059816522 cites W2020966007 @default.
• W2059816522 cites W2022126715 @default.
• W2059816522 cites W2022677304 @default.
• W2059816522 cites W2034875873 @default.
• W2059816522 cites W2047480444 @default.
• W2059816522 cites W2051038137 @default.
• W2059816522 cites W2061890028 @default.
• W2059816522 cites W2063445385 @default.
• W2059816522 cites W2066292941 @default.
• W2059816522 cites W2068076697 @default.
• W2059816522 cites W2074729249 @default.
• W2059816522 cites W2078846682 @default.
• W2059816522 cites W2088564782 @default.
• W2059816522 cites W2089832032 @default.
• W2059816522 cites W2095712895 @default.
• W2059816522 cites W2104991761 @default.
• W2059816522 cites W2155178339 @default.
• W2059816522 cites W2311724061 @default.
• W2059816522 cites W2441119074 @default.
• W2059816522 cites W3213292632 @default.
• W2059816522 doi "https://doi.org/10.3168/jds.s0022-0302(93)77677-8" @default.
• W2059816522 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8270681" @default.
• W2059816522 hasPublicationYear "1993" @default.
• W2059816522 type Work @default.
• W2059816522 sameAs 2059816522 @default.
• W2059816522 citedByCount "21" @default.
• W2059816522 crossrefType "journal-article" @default.
• W2059816522 hasAuthorship W2059816522A5014699274 @default.
• W2059816522 hasAuthorship W2059816522A5017386242 @default.
• W2059816522 hasAuthorship W2059816522A5031061558 @default.
• W2059816522 hasAuthorship W2059816522A5045814298 @default.
• W2059816522 hasAuthorship W2059816522A5053694306 @default.
• W2059816522 hasAuthorship W2059816522A5057791131 @default.
• W2059816522 hasBestOaLocation W20598165221 @default.
• W2059816522 hasConcept C104317684 @default.
• W2059816522 hasConcept C110758877 @default.
• W2059816522 hasConcept C134018914 @default.
• W2059816522 hasConcept C153911025 @default.
• W2059816522 hasConcept C16685009 @default.
• W2059816522 hasConcept C196843134 @default.
• W2059816522 hasConcept C202751555 @default.
• W2059816522 hasConcept C2777129507 @default.
• W2059816522 hasConcept C2778177303 @default.
• W2059816522 hasConcept C28748434 @default.
• W2059816522 hasConcept C49105822 @default.
• W2059816522 hasConcept C54355233 @default.
• W2059816522 hasConcept C62525196 @default.
• W2059816522 hasConcept C71924100 @default.
• W2059816522 hasConcept C86803240 @default.
• W2059816522 hasConcept C87073359 @default.
• W2059816522 hasConceptScore W2059816522C104317684 @default.
• W2059816522 hasConceptScore W2059816522C110758877 @default.
• W2059816522 hasConceptScore W2059816522C134018914 @default.
• W2059816522 hasConceptScore W2059816522C153911025 @default.
• W2059816522 hasConceptScore W2059816522C16685009 @default.
• W2059816522 hasConceptScore W2059816522C196843134 @default.
• W2059816522 hasConceptScore W2059816522C202751555 @default.
• W2059816522 hasConceptScore W2059816522C2777129507 @default.
• W2059816522 hasConceptScore W2059816522C2778177303 @default.
• W2059816522 hasConceptScore W2059816522C28748434 @default.
• W2059816522 hasConceptScore W2059816522C49105822 @default.
• W2059816522 hasConceptScore W2059816522C54355233 @default.
• W2059816522 hasConceptScore W2059816522C62525196 @default.
• W2059816522 hasConceptScore W2059816522C71924100 @default.
• W2059816522 hasConceptScore W2059816522C86803240 @default.
• W2059816522 hasConceptScore W2059816522C87073359 @default.
• W2059816522 hasLocation W20598165221 @default.
• W2059816522 hasLocation W20598165222 @default.
• W2059816522 hasOpenAccess W2059816522 @default.
• W2059816522 hasPrimaryLocation W20598165221 @default.
• W2059816522 hasRelatedWork W1970811629 @default.
• W2059816522 hasRelatedWork W2004218199 @default.
• W2059816522 hasRelatedWork W2036028667 @default.
• W2059816522 hasRelatedWork W2059816522 @default.
• W2059816522 hasRelatedWork W2060146325 @default.
• W2059816522 hasRelatedWork W2088252231 @default.
• W2059816522 hasRelatedWork W2099535287 @default.
• W2059816522 hasRelatedWork W2367018321 @default.
• W2059816522 hasRelatedWork W2378339968 @default.
• W2059816522 hasRelatedWork W267988810 @default.
• W2059816522 isParatext "false" @default.
• W2059816522 isRetracted "false" @default.
• W2059816522 magId "2059816522" @default.

• next