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- W2059875550 abstract "Abstract A genetic and physical map of Escherichia coli plasmid R538-1 was constructed using restriction endonucleases and molecular cloning techniques. R538-1 DNA was cleaved into 12 fragments by endonuclease · R · Eco RI, 6 fragments by endonuclease R · Hin dIII, and 3 fragments by endonuclease R · Bam HI. The order of these fragments was determined by standard restriction fragment mapping techniques. Endo · R · Eco RI, endo · R · Hin dIII, endo · R · Bam HI, and endo · R · Pst I fragments obtained from R538-1 and ColE1-derived plasmids (pMB9, ColE1Ap r , and pBR322) were ligated in vitro and used to transform E. coli C600. Transformants were selected for antibiotic resistance markers carried by R538-1. Analysis of the R538-1 fragments contained in these hybrid plasmids permitted the construction of a genetic map of the R538-1 plasmid. The genetic map of this plasmid is very similar to that of plasmid R100." @default.
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- W2059875550 date "1978-06-01" @default.
- W2059875550 modified "2023-10-14" @default.
- W2059875550 title "Molecular cloning of restriction fragments and construction of a physical and genetic map of the Escherichia coli plasmid R538-1" @default.
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- W2059875550 doi "https://doi.org/10.1016/0147-619x(78)90054-9" @default.
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