Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059896265> ?p ?o ?g. }
- W2059896265 endingPage "2251" @default.
- W2059896265 startingPage "2241" @default.
- W2059896265 abstract "Abstract A preparation of recombinant birch pollen allergen of Betula verrucosa isoform 1a (Bet v 1a) containing chemically modified (carbamylated) variants has been analyzed by CZE and CIEF. In CZE, employing a 100 mmol/L MES buffer at pH 6.50, with 0.4 mmol/L tetraethylenepentamine (TEPA) added, allowed for the resolution of 17 protein fractions. The CIEF profiling of the allergen preparation required a combination of a wide‐pH‐range carrier ampholyte (CA) of pH 3–10 with two narrow‐range CAs of pH 5–6 and 5–7. For CIEF, 91 mmol/L of glycine at pH 2.12 and 20 mmol/L of CHES at pH 10.00 were applied as anolyte and catholyte, respectively. The generated pH gradient was nonlinear with a flat slope for pH 4–6, thus providing an improved resolution. In CIEF, up to 18 protein fractions were distinguished as well. The p I of the target allergen Bet v 1a was 4.9 as determined by means of two p I marker compounds flanking the allergen. Relative purity of the target allergen within the preparation containing carbamylated variants was in accordance for both separation systems and varied between 40.7 and 42.8%." @default.
- W2059896265 created "2016-06-24" @default.
- W2059896265 creator A5039467765 @default.
- W2059896265 creator A5050617437 @default.
- W2059896265 creator A5091374943 @default.
- W2059896265 date "2007-07-01" @default.
- W2059896265 modified "2023-10-18" @default.
- W2059896265 title "Profiling recombinant major birch pollen allergen Bet v 1a and carbamylated variants with CZE and CIEF" @default.
- W2059896265 cites W189446590 @default.
- W2059896265 cites W1966199235 @default.
- W2059896265 cites W1982127463 @default.
- W2059896265 cites W1985391925 @default.
- W2059896265 cites W1988482874 @default.
- W2059896265 cites W1989861229 @default.
- W2059896265 cites W1993255151 @default.
- W2059896265 cites W1994359117 @default.
- W2059896265 cites W1996979312 @default.
- W2059896265 cites W1997198229 @default.
- W2059896265 cites W1997944974 @default.
- W2059896265 cites W1998786130 @default.
- W2059896265 cites W2002667781 @default.
- W2059896265 cites W2004935008 @default.
- W2059896265 cites W2005732586 @default.
- W2059896265 cites W2006777849 @default.
- W2059896265 cites W2009476264 @default.
- W2059896265 cites W2011493463 @default.
- W2059896265 cites W2015147436 @default.
- W2059896265 cites W2020732219 @default.
- W2059896265 cites W2022339017 @default.
- W2059896265 cites W2029339919 @default.
- W2059896265 cites W2029809864 @default.
- W2059896265 cites W2030172614 @default.
- W2059896265 cites W2033465911 @default.
- W2059896265 cites W2034562096 @default.
- W2059896265 cites W2040300887 @default.
- W2059896265 cites W2043229877 @default.
- W2059896265 cites W2046200473 @default.
- W2059896265 cites W2046600138 @default.
- W2059896265 cites W2051079878 @default.
- W2059896265 cites W2052233059 @default.
- W2059896265 cites W2052384160 @default.
- W2059896265 cites W2052457044 @default.
- W2059896265 cites W2052507142 @default.
- W2059896265 cites W2052638754 @default.
- W2059896265 cites W2057364524 @default.
- W2059896265 cites W2060706382 @default.
- W2059896265 cites W2069177428 @default.
- W2059896265 cites W2073317144 @default.
- W2059896265 cites W2075019839 @default.
- W2059896265 cites W2082474780 @default.
- W2059896265 cites W2083842525 @default.
- W2059896265 cites W2083863843 @default.
- W2059896265 cites W2088252563 @default.
- W2059896265 cites W2090176404 @default.
- W2059896265 cites W2090738309 @default.
- W2059896265 cites W2092295247 @default.
- W2059896265 cites W2094606373 @default.
- W2059896265 cites W2095653057 @default.
- W2059896265 cites W2098524113 @default.
- W2059896265 cites W2101936254 @default.
- W2059896265 cites W2110068169 @default.
- W2059896265 cites W2128140264 @default.
- W2059896265 cites W2129401772 @default.
- W2059896265 cites W2132366094 @default.
- W2059896265 cites W2142104533 @default.
- W2059896265 cites W2902622046 @default.
- W2059896265 cites W4232072950 @default.
- W2059896265 cites W4241180012 @default.
- W2059896265 cites W4243278371 @default.
- W2059896265 cites W2126672079 @default.
- W2059896265 doi "https://doi.org/10.1002/elps.200600764" @default.
- W2059896265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17538926" @default.
- W2059896265 hasPublicationYear "2007" @default.
- W2059896265 type Work @default.
- W2059896265 sameAs 2059896265 @default.
- W2059896265 citedByCount "15" @default.
- W2059896265 countsByYear W20598962652013 @default.
- W2059896265 countsByYear W20598962652014 @default.
- W2059896265 countsByYear W20598962652016 @default.
- W2059896265 countsByYear W20598962652017 @default.
- W2059896265 countsByYear W20598962652018 @default.
- W2059896265 countsByYear W20598962652020 @default.
- W2059896265 countsByYear W20598962652023 @default.
- W2059896265 crossrefType "journal-article" @default.
- W2059896265 hasAuthorship W2059896265A5039467765 @default.
- W2059896265 hasAuthorship W2059896265A5050617437 @default.
- W2059896265 hasAuthorship W2059896265A5091374943 @default.
- W2059896265 hasConcept C104317684 @default.
- W2059896265 hasConcept C124815738 @default.
- W2059896265 hasConcept C138268822 @default.
- W2059896265 hasConcept C154945302 @default.
- W2059896265 hasConcept C185592680 @default.
- W2059896265 hasConcept C203014093 @default.
- W2059896265 hasConcept C207480886 @default.
- W2059896265 hasConcept C2780510475 @default.
- W2059896265 hasConcept C40767141 @default.
- W2059896265 hasConcept C41008148 @default.
- W2059896265 hasConcept C43617362 @default.