Matches in SemOpenAlex for { <https://semopenalex.org/work/W2059912902> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2059912902 abstract "Abstract The over-expression of foreign proteins imposes metabolic burden on host strains that may lead to reduced cell growth and even yield of target protein. We investigated dependency of co-expression effects of stress-induced non-specific DNA-binding protein, Dps, on cell growth and foreign protein expression according to solubility in Escherichia coli . Dps co-expression showed clear distinct effects according to solubility of target proteins. Under co-expression of recombinant Dps, cell growth for the strain expressing baculoviral polyhedrin (Polh)-green fluorescent protein (GFP) fusion protein or human interleukin-2 (hIL-2) that were expressed as insoluble inclusion body had tendency to decline slightly compared to each Dps non-expressing strain. However, cell growth for the strain expressing soluble GFP or mussel adhesive protein type 5 (Mgfp-5) was somewhat increased. While Dps co-expression had somewhat negative effects on expression of soluble protein, it showed huge impacts on product yield of insoluble proteins (1.6–1.8-fold for Polh-GFP and 4–5-fold for hIL-2). Therefore, it was obvious that co-expression of Dps has different effects on foreign protein production according to solubility. Proteomic analyses revealed that Dps co-expression induced significantly different global patterns through interaction with target foreign protein according to target solubility. These global pattern alterations might be favorable for production of insoluble foreign proteins indirectly." @default.
- W2059912902 created "2016-06-24" @default.
- W2059912902 creator A5055650324 @default.
- W2059912902 creator A5083066637 @default.
- W2059912902 date "2006-07-01" @default.
- W2059912902 modified "2023-09-24" @default.
- W2059912902 title "Solubility dependency of co-expression effects of stress-induced protein Dps on foreign protein expression in Escherichia coli" @default.
- W2059912902 cites W156406831 @default.
- W2059912902 cites W1572008831 @default.
- W2059912902 cites W1781902555 @default.
- W2059912902 cites W1897176773 @default.
- W2059912902 cites W1931534490 @default.
- W2059912902 cites W1965987341 @default.
- W2059912902 cites W1986271212 @default.
- W2059912902 cites W1992592716 @default.
- W2059912902 cites W1994187511 @default.
- W2059912902 cites W2024453737 @default.
- W2059912902 cites W2061831461 @default.
- W2059912902 cites W2065762927 @default.
- W2059912902 cites W2070392843 @default.
- W2059912902 cites W2073626488 @default.
- W2059912902 cites W2075853257 @default.
- W2059912902 cites W2077374889 @default.
- W2059912902 cites W2087732324 @default.
- W2059912902 cites W2087823077 @default.
- W2059912902 cites W2089588701 @default.
- W2059912902 cites W2111553015 @default.
- W2059912902 cites W2114670018 @default.
- W2059912902 cites W2119936296 @default.
- W2059912902 cites W2122952856 @default.
- W2059912902 cites W2127558534 @default.
- W2059912902 cites W2128996981 @default.
- W2059912902 cites W2138840910 @default.
- W2059912902 cites W2143533963 @default.
- W2059912902 cites W2153150816 @default.
- W2059912902 cites W2155031737 @default.
- W2059912902 cites W2156185271 @default.
- W2059912902 cites W2162102151 @default.
- W2059912902 cites W2177384235 @default.
- W2059912902 doi "https://doi.org/10.1016/j.enzmictec.2005.11.040" @default.
- W2059912902 hasPublicationYear "2006" @default.
- W2059912902 type Work @default.
- W2059912902 sameAs 2059912902 @default.
- W2059912902 citedByCount "4" @default.
- W2059912902 countsByYear W20599129022020 @default.
- W2059912902 crossrefType "journal-article" @default.
- W2059912902 hasAuthorship W2059912902A5055650324 @default.
- W2059912902 hasAuthorship W2059912902A5083066637 @default.
- W2059912902 hasConcept C104317684 @default.
- W2059912902 hasConcept C123894998 @default.
- W2059912902 hasConcept C139447449 @default.
- W2059912902 hasConcept C142613039 @default.
- W2059912902 hasConcept C155574463 @default.
- W2059912902 hasConcept C178790620 @default.
- W2059912902 hasConcept C185592680 @default.
- W2059912902 hasConcept C203750385 @default.
- W2059912902 hasConcept C40767141 @default.
- W2059912902 hasConcept C547475151 @default.
- W2059912902 hasConcept C55493867 @default.
- W2059912902 hasConcept C86803240 @default.
- W2059912902 hasConceptScore W2059912902C104317684 @default.
- W2059912902 hasConceptScore W2059912902C123894998 @default.
- W2059912902 hasConceptScore W2059912902C139447449 @default.
- W2059912902 hasConceptScore W2059912902C142613039 @default.
- W2059912902 hasConceptScore W2059912902C155574463 @default.
- W2059912902 hasConceptScore W2059912902C178790620 @default.
- W2059912902 hasConceptScore W2059912902C185592680 @default.
- W2059912902 hasConceptScore W2059912902C203750385 @default.
- W2059912902 hasConceptScore W2059912902C40767141 @default.
- W2059912902 hasConceptScore W2059912902C547475151 @default.
- W2059912902 hasConceptScore W2059912902C55493867 @default.
- W2059912902 hasConceptScore W2059912902C86803240 @default.
- W2059912902 hasLocation W20599129021 @default.
- W2059912902 hasOpenAccess W2059912902 @default.
- W2059912902 hasPrimaryLocation W20599129021 @default.
- W2059912902 hasRelatedWork W1521735421 @default.
- W2059912902 hasRelatedWork W1976943917 @default.
- W2059912902 hasRelatedWork W2003338675 @default.
- W2059912902 hasRelatedWork W2088132348 @default.
- W2059912902 hasRelatedWork W2114362838 @default.
- W2059912902 hasRelatedWork W2164390141 @default.
- W2059912902 hasRelatedWork W2530389131 @default.
- W2059912902 hasRelatedWork W2543799468 @default.
- W2059912902 hasRelatedWork W2574203877 @default.
- W2059912902 hasRelatedWork W4220756668 @default.
- W2059912902 isParatext "false" @default.
- W2059912902 isRetracted "false" @default.
- W2059912902 magId "2059912902" @default.
- W2059912902 workType "article" @default.