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- W2059944076 abstract "Background: We developed an HPLC-MS/MS method to quantify tamoxifen (2.5–250 ng/ml) and its metabolite (Z)-endoxifen (0.5–50 ng/ml) in dried blood spots. Results: Extraction recovery of both analytes from Whatman DMPK-A cards was 100% and consistent over time, however, recovery of (Z)-endoxifen from Whatman 903 cards was incomplete and increased upon storage. When SDS, a constituent of the DMPK-A coating, was present during the extraction, recovery improved. The method using DMPK-A cards was validated using bioanalytical guidelines. Additionally, influence of haematocrit (0.29–0.48 L/L), spot volume (20–50µl) and homogeneity was within limits and both analytes were stable in DBS for at least 4 months. Conclusions: The method for the quantification of tamoxifen and (Z)-endoxifen in DBS collected on DMPK-A cards was successfully validated." @default.
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- W2059944076 date "2014-11-01" @default.
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- W2059944076 title "Determination of tamoxifen and endoxifen in dried blood spots using LC–MS/MS and the effect of coated DBS cards on recovery and matrix effects" @default.
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- W2059944076 doi "https://doi.org/10.4155/bio.14.157" @default.
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