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- W2059969653 abstract "Thin spin-coated films of polystyrene-block-poly(tert-butyl acrylate) (PS690-b-PtBA1210) on various substrates are introduced as versatile, robust reactive platform for the immobilization of (bio)molecules for the fabrication of tailored biointerfaces. The films are characterized by high stability and (bio)reactivity due to the presence of a glassy PS and a reactive PtBA block, respectively. The selective deprotection of the tert-butyl-ester groups in the PtBA skin layer by hydrolysis under acidic conditions, the activation with N-hydroxysuccinimide and the subsequent derivatization with amino functionalized (bio)molecules were investigated. Based on contact angle, FTIR spectroscopy and XPS, fluorescence microscopy and AFM data, it was shown that the (bio)molecules were coupled covalently to the polymer films and that high molecular coverages up to ∼2.4 poly(ethylene glycol) (PEG) molecules per nm2 (Mn = 500 g/mol) were obtained. Organic dyes, polyamidoamine dendrimers, polypeptides, proteins and amino end-functionalized DNA were efficiently and homogeneously immobilized on the PS-PtBA platforms. Grafting of ω-amino functionalized PEG afforded surfaces with substantially reduced non-specific adsorption of proteins and DNA. Owing to the glassy nature of PS and the covalent amide linkages, the derivatized films showed excellent stability under a broad range of processing conditions. Finally, the viability of PS690-b-PtBA1210 platforms as versatile biointerfaces was demonstrated in DNA hybridization experiments, as well as cell-surface interaction studies using pancreatic cancer and K562 cells." @default.
- W2059969653 created "2016-06-24" @default.
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- W2059969653 date "2007-06-01" @default.
- W2059969653 modified "2023-10-17" @default.
- W2059969653 title "Tailored interfaces for biosensors and cell-surface interaction studies via activation and derivatization of polystyrene-block-poly(tert-butyl acrylate) thin films" @default.
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- W2059969653 cites W1974018656 @default.
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- W2059969653 cites W1974560628 @default.
- W2059969653 cites W1977308353 @default.
- W2059969653 cites W1977943530 @default.
- W2059969653 cites W1977970573 @default.
- W2059969653 cites W1980416838 @default.
- W2059969653 cites W1981028296 @default.
- W2059969653 cites W1984267955 @default.
- W2059969653 cites W1984315018 @default.
- W2059969653 cites W1988847233 @default.
- W2059969653 cites W1994670963 @default.
- W2059969653 cites W1994869073 @default.
- W2059969653 cites W1995829317 @default.
- W2059969653 cites W1996767514 @default.
- W2059969653 cites W2005324943 @default.
- W2059969653 cites W2006657990 @default.
- W2059969653 cites W2009437526 @default.
- W2059969653 cites W2010782361 @default.
- W2059969653 cites W2011905004 @default.
- W2059969653 cites W2012368513 @default.
- W2059969653 cites W2012644189 @default.
- W2059969653 cites W2013343352 @default.
- W2059969653 cites W2015165584 @default.
- W2059969653 cites W2017330811 @default.
- W2059969653 cites W2020978939 @default.
- W2059969653 cites W2021517658 @default.
- W2059969653 cites W2023135762 @default.
- W2059969653 cites W2023766971 @default.
- W2059969653 cites W2032174676 @default.
- W2059969653 cites W2034426138 @default.
- W2059969653 cites W2034882563 @default.
- W2059969653 cites W2040932720 @default.
- W2059969653 cites W2041212700 @default.
- W2059969653 cites W2041985764 @default.
- W2059969653 cites W2042205161 @default.
- W2059969653 cites W2046642433 @default.
- W2059969653 cites W2048291801 @default.
- W2059969653 cites W2050777364 @default.
- W2059969653 cites W2052453884 @default.
- W2059969653 cites W2055079612 @default.
- W2059969653 cites W2059542554 @default.
- W2059969653 cites W2067011627 @default.
- W2059969653 cites W2069960852 @default.
- W2059969653 cites W2076549842 @default.
- W2059969653 cites W2081173982 @default.
- W2059969653 cites W2081688312 @default.
- W2059969653 cites W2082972185 @default.
- W2059969653 cites W2085738178 @default.
- W2059969653 cites W2088267337 @default.
- W2059969653 cites W2092913048 @default.
- W2059969653 cites W2104025895 @default.
- W2059969653 cites W2108452517 @default.
- W2059969653 cites W2113280649 @default.
- W2059969653 cites W2115273449 @default.
- W2059969653 cites W2125341431 @default.
- W2059969653 cites W2128188359 @default.
- W2059969653 cites W2130093942 @default.
- W2059969653 cites W2132551021 @default.
- W2059969653 cites W2132655146 @default.
- W2059969653 cites W2133482050 @default.
- W2059969653 cites W2134130835 @default.
- W2059969653 cites W2135470343 @default.
- W2059969653 cites W2137783186 @default.
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- W2059969653 cites W2156246479 @default.
- W2059969653 cites W2166086274 @default.
- W2059969653 cites W4247309562 @default.
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- W2059969653 doi "https://doi.org/10.1016/j.eurpolymj.2007.03.021" @default.
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