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• W2060009917 endingPage "7131" @default.
• W2060009917 startingPage "7117" @default.
• W2060009917 abstract "Translocation of bacterial toxins or effectors into host cells using the type III secretion (T3S) system is a conserved mechanism shared by many Gram-negative pathogens. Pseudomonas aeruginosa injects different proteins across the plasma membrane of target cells, altering the normal metabolism of the host. Protein translocation presumably occurs through a proteinaceous transmembrane pore formed by two T3S secreted protein translocators, PopB and PopD. Unfolded translocators are secreted through the T3S needle prior to insertion into the target membrane. Purified PopB and PopD form pores in model membranes. However, their tendency to form heterogeneous aggregates in solution had hampered the analysis of how these proteins undergo the transition from a denatured state to a membrane-inserted state. Translocators were purified as stable complexes with the cognate chaperone PcrH and isolated from the chaperone using 6 M urea. We report here the assembly of stable transmembrane pores by dilution of urea-denatured translocators in the presence of membranes. PopB and PopD spontaneously bound liposomes containing anionic phospholipids and cholesterol in a pH-dependent manner as observed by two independent assays, time-resolved Förster resonance energy transfer and sucrose-step gradient ultracentrifugation. Using Bodipy-labeled proteins, we found that PopB interacts with PopD on the membrane surface as determined by excitation energy migration and fluorescence quenching. Stable transmembrane pores are more efficiently assembled at pH <5.0, suggesting that acidic residues might be involved in the initial membrane binding and/or insertion. Altogether, the experimental setup described here represents an efficient method for the reconstitution and analysis of membrane-inserted translocators." @default.
• W2060009917 created "2016-06-24" @default.
• W2060009917 creator A5003385998 @default.
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• W2060009917 creator A5056897795 @default.
• W2060009917 creator A5064035517 @default.
• W2060009917 date "2011-07-26" @default.
• W2060009917 modified "2023-10-17" @default.
• W2060009917 title "Efficient Isolation of <i>Pseudomonas aeruginosa</i> Type III Secretion Translocators and Assembly of Heteromeric Transmembrane Pores in Model Membranes" @default.
• W2060009917 cites W1494429407 @default.
• W2060009917 cites W1497273660 @default.
• W2060009917 cites W1511786918 @default.
• W2060009917 cites W1546885641 @default.
• W2060009917 cites W1565489479 @default.
• W2060009917 cites W1605208918 @default.
• W2060009917 cites W1821732051 @default.
• W2060009917 cites W1964051095 @default.
• W2060009917 cites W1967399503 @default.
• W2060009917 cites W1971413967 @default.
• W2060009917 cites W1972382160 @default.
• W2060009917 cites W1976224933 @default.
• W2060009917 cites W1977271009 @default.
• W2060009917 cites W1980355157 @default.
• W2060009917 cites W1986761673 @default.
• W2060009917 cites W1992162228 @default.
• W2060009917 cites W1994369382 @default.
• W2060009917 cites W1996341064 @default.
• W2060009917 cites W2001276190 @default.
• W2060009917 cites W2003551345 @default.
• W2060009917 cites W2004668209 @default.
• W2060009917 cites W2006872113 @default.
• W2060009917 cites W2007142983 @default.
• W2060009917 cites W2014749312 @default.
• W2060009917 cites W2017276607 @default.
• W2060009917 cites W2017550398 @default.
• W2060009917 cites W2018054206 @default.
• W2060009917 cites W2018271515 @default.
• W2060009917 cites W2019228627 @default.
• W2060009917 cites W2019333610 @default.
• W2060009917 cites W2021071631 @default.
• W2060009917 cites W2034548105 @default.
• W2060009917 cites W2037099987 @default.
• W2060009917 cites W2038131958 @default.
• W2060009917 cites W2045421528 @default.
• W2060009917 cites W2052797725 @default.
• W2060009917 cites W2055486660 @default.
• W2060009917 cites W2056186055 @default.
• W2060009917 cites W2057468776 @default.
• W2060009917 cites W2062106725 @default.
• W2060009917 cites W2063024547 @default.
• W2060009917 cites W2069520354 @default.
• W2060009917 cites W2075162871 @default.
• W2060009917 cites W2077049678 @default.
• W2060009917 cites W2082022392 @default.
• W2060009917 cites W2085837389 @default.
• W2060009917 cites W2086447005 @default.
• W2060009917 cites W2087528475 @default.
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• W2060009917 cites W2090821297 @default.
• W2060009917 cites W2098280552 @default.
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• W2060009917 cites W2113348899 @default.
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• W2060009917 cites W2146089032 @default.
• W2060009917 cites W2148956213 @default.
• W2060009917 cites W2150315067 @default.
• W2060009917 cites W2153266303 @default.
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• W2060009917 doi "https://doi.org/10.1021/bi200905x" @default.
• W2060009917 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3171962" @default.
• W2060009917 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21770428" @default.
• W2060009917 hasPublicationYear "2011" @default.
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