FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060011275> ?p ?o ?g. }

• W2060011275 endingPage "95" @default.
• W2060011275 startingPage "90" @default.
• W2060011275 abstract "Following the introduction of potent aromatase inhibitors for the treatment of breast cancer patients, highly sensitive methods have become mandatory to evaluate the influence of these drugs on plasma estrogen levels. Commercially available kits for estrogen measurements are not suitable for these kinds of evaluations due to their detection limits that are close to baseline estrogen levels in postmenopausal women. We describe here an optimised radioimmunoassay suitable for the simultaneous measurement of plasma estrone (E1), estradiol (E2) and estrone sulfate (E1S) levels in the ultra-low range. Following incubation with [3H]-labelled estrogens as internal standards, crude estrogen fractions were separated by ether extraction. The E1S fraction was hydrolysed with sulfatase followed by eluation on a Sephadex column. Free estrogens (E1, E2) were separated by chromatography (LH-20). Estrone and E1S (following hydrolysis) were converted into E2, and each estrogen fraction was measured by the same highly sensitive and specific radioimmunoassay using estradiol-6-(O-carboxymethyl)-oximino-2-(2-[125I]-iodo-histamine) as ligand. Although several purification steps were involved, the internal recovery values for tritiated estrogens were found to be 88%, 90%, and 49% for E1, E2 and E1S, respectively. The intra-assay coefficient of variation was <5% for all recovery measurements. The detection limits were calculated following repeated blank measurements and found to be 1.14 pmol/L for E1, 0.67 pmol/L for E2, and 0.55 pmol/L for E1S, respectively. The intra-assay coefficient of variation (CV) was found to be 3.4% for E1, 5.1% for E2 and 6.1% for E1S, while the inter-assay CV was 13.6%, 7.6% and 7.5% for E1, E2, and E1S, respectively. Considering normal plasma levels for E2 (15 pmol/L), E1 (80 pmol/L) and E1S (400 pmol/L) in postmenopausal women, the method allows theoretically to detect suppression of plasma E2, E1 and E1S levels by 95.5%, 98.6% and 99.9% when starting from average, normal postmenopausal levels. Thus, the method presented here is to our knowledge the currently most sensitive assay available for plasma estrogen measurements in the ultra-low range and, as such, a reliable tool for a proper evaluation of potent aromatase inhibitors and other potential drugs influencing on plasma estrogen levels." @default.
• W2060011275 created "2016-06-24" @default.
• W2060011275 creator A5008138209 @default.
• W2060011275 creator A5031286476 @default.
• W2060011275 creator A5032368110 @default.
• W2060011275 creator A5035837487 @default.
• W2060011275 creator A5067278670 @default.
• W2060011275 date "2008-03-01" @default.
• W2060011275 modified "2023-10-16" @default.
• W2060011275 title "An optimised, highly sensitive radioimmunoassay for the simultaneous measurement of estrone, estradiol and estrone sulfate in the ultra-low range in human plasma samples" @default.
• W2060011275 cites W1453600927 @default.
• W2060011275 cites W1531964529 @default.
• W2060011275 cites W17379279 @default.
• W2060011275 cites W1754579018 @default.
• W2060011275 cites W1918073772 @default.
• W2060011275 cites W1964172805 @default.
• W2060011275 cites W1980417800 @default.
• W2060011275 cites W1992405425 @default.
• W2060011275 cites W1994207027 @default.
• W2060011275 cites W1995876524 @default.
• W2060011275 cites W1998401636 @default.
• W2060011275 cites W2009336450 @default.
• W2060011275 cites W2047038635 @default.
• W2060011275 cites W2050847082 @default.
• W2060011275 cites W2068057248 @default.
• W2060011275 cites W2101881391 @default.
• W2060011275 cites W2122095366 @default.
• W2060011275 cites W2123130972 @default.
• W2060011275 cites W2148777110 @default.
• W2060011275 cites W2151921197 @default.
• W2060011275 cites W2163688875 @default.
• W2060011275 cites W2263139599 @default.
• W2060011275 cites W2508460892 @default.
• W2060011275 cites W4230176650 @default.
• W2060011275 doi "https://doi.org/10.1016/j.jsbmb.2007.12.011" @default.
• W2060011275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18242079" @default.
• W2060011275 hasPublicationYear "2008" @default.
• W2060011275 type Work @default.
• W2060011275 sameAs 2060011275 @default.
• W2060011275 citedByCount "61" @default.
• W2060011275 countsByYear W20600112752012 @default.
• W2060011275 countsByYear W20600112752013 @default.
• W2060011275 countsByYear W20600112752014 @default.
• W2060011275 countsByYear W20600112752015 @default.
• W2060011275 countsByYear W20600112752016 @default.
• W2060011275 countsByYear W20600112752017 @default.
• W2060011275 countsByYear W20600112752018 @default.
• W2060011275 countsByYear W20600112752019 @default.
• W2060011275 countsByYear W20600112752020 @default.
• W2060011275 countsByYear W20600112752021 @default.
• W2060011275 countsByYear W20600112752022 @default.
• W2060011275 crossrefType "journal-article" @default.
• W2060011275 hasAuthorship W2060011275A5008138209 @default.
• W2060011275 hasAuthorship W2060011275A5031286476 @default.
• W2060011275 hasAuthorship W2060011275A5032368110 @default.
• W2060011275 hasAuthorship W2060011275A5035837487 @default.
• W2060011275 hasAuthorship W2060011275A5067278670 @default.
• W2060011275 hasConcept C119128265 @default.
• W2060011275 hasConcept C134018914 @default.
• W2060011275 hasConcept C176685330 @default.
• W2060011275 hasConcept C185592680 @default.
• W2060011275 hasConcept C2777164284 @default.
• W2060011275 hasConcept C2778656385 @default.
• W2060011275 hasConcept C2779149875 @default.
• W2060011275 hasConcept C43617362 @default.
• W2060011275 hasConcept C55493867 @default.
• W2060011275 hasConcept C86803240 @default.
• W2060011275 hasConcept C89838059 @default.
• W2060011275 hasConceptScore W2060011275C119128265 @default.
• W2060011275 hasConceptScore W2060011275C134018914 @default.
• W2060011275 hasConceptScore W2060011275C176685330 @default.
• W2060011275 hasConceptScore W2060011275C185592680 @default.
• W2060011275 hasConceptScore W2060011275C2777164284 @default.
• W2060011275 hasConceptScore W2060011275C2778656385 @default.
• W2060011275 hasConceptScore W2060011275C2779149875 @default.
• W2060011275 hasConceptScore W2060011275C43617362 @default.
• W2060011275 hasConceptScore W2060011275C55493867 @default.
• W2060011275 hasConceptScore W2060011275C86803240 @default.
• W2060011275 hasConceptScore W2060011275C89838059 @default.
• W2060011275 hasIssue "1-2" @default.
• W2060011275 hasLocation W20600112751 @default.
• W2060011275 hasLocation W20600112752 @default.
• W2060011275 hasOpenAccess W2060011275 @default.
• W2060011275 hasPrimaryLocation W20600112751 @default.
• W2060011275 hasRelatedWork W1966332474 @default.
• W2060011275 hasRelatedWork W1975619680 @default.
• W2060011275 hasRelatedWork W1991019901 @default.
• W2060011275 hasRelatedWork W2006379730 @default.
• W2060011275 hasRelatedWork W2023202574 @default.
• W2060011275 hasRelatedWork W204340081 @default.
• W2060011275 hasRelatedWork W2059865620 @default.
• W2060011275 hasRelatedWork W2074815152 @default.
• W2060011275 hasRelatedWork W2079250078 @default.
• W2060011275 hasRelatedWork W2158604980 @default.
• W2060011275 hasVolume "109" @default.
• W2060011275 isParatext "false" @default.
• W2060011275 isRetracted "false" @default.
• W2060011275 magId "2060011275" @default.

• next