FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060017723> ?p ?o ?g. }

• W2060017723 abstract "Kinetic, chromatographic and electrophoretic studies of Chromatium hydrogenase show the existence in vitro of two different activities (I and II). The two hydrogenases exhibit different kinetic parameters and properties. Using reduced methyl viologen, Km and [S]0.5 values of about 20 microM and 360 microM were calculated for the hydrogenases I and II, respectively. Hill plots revealed that hydrogenase I followed classical hyperbolic (Michaelis-Menten) kinetics. However, a Hill coefficient (h = 0.68) indicating non-hyperbolic kinetics could be shown for hydrogenase II. After several purification steps hydrogenase II still showed kinetics typical of the action of either (a) two enzymes each of which shows Michaelis-Menten kinetics but with different substrate affinities or (b) only one enzyme which shows apparent negative cooperative regulation. The molecular weights of the hydrogenases were about 37,000 (I) and 55,000 (II) when determined by gel filtration. Sodium dodecyl sulphate/polyacrylamide gel electrophoresis revealed that both enzymes give a coincidental single protein band with the same relative mobility indicating a molecular weight of 31,000. Both hydrogenases were able to catalyse the reversible activation of H2 in the presence of artificial electron carriers but with different rates, hydrogenase II being much more active in the H2-uptake mode. The kinetic properties and molecular weight of hydrogenase II are partially modified by high ionic strength resulting in an increased substrate affinity and Hill coefficient and thus resembling hydrogenase I. These results are interpreted as due to the existence in vitro of monomeric and dimeric forms of Chromatium hydrogenase." @default.
• W2060017723 created "2016-06-24" @default.
• W2060017723 creator A5032033695 @default.
• W2060017723 creator A5045358445 @default.
• W2060017723 creator A5048149002 @default.
• W2060017723 creator A5085379313 @default.
• W2060017723 date "2005-03-03" @default.
• W2060017723 modified "2023-09-25" @default.
• W2060017723 title "Isolation of Two Hydrogenase Activities in Chromatium" @default.
• W2060017723 cites W156926467 @default.
• W2060017723 cites W1750773183 @default.
• W2060017723 cites W1781980958 @default.
• W2060017723 cites W1939912932 @default.
• W2060017723 cites W1971781666 @default.
• W2060017723 cites W1979204404 @default.
• W2060017723 cites W1990241814 @default.
• W2060017723 cites W2009652285 @default.
• W2060017723 cites W2011219512 @default.
• W2060017723 cites W2027369906 @default.
• W2060017723 cites W2030637960 @default.
• W2060017723 cites W2036145567 @default.
• W2060017723 cites W2043333515 @default.
• W2060017723 cites W2043981229 @default.
• W2060017723 cites W2048997178 @default.
• W2060017723 cites W2050553755 @default.
• W2060017723 cites W2055735042 @default.
• W2060017723 cites W2074581799 @default.
• W2060017723 cites W2084856012 @default.
• W2060017723 cites W2090764568 @default.
• W2060017723 cites W2093645948 @default.
• W2060017723 cites W2100837269 @default.
• W2060017723 cites W2111301002 @default.
• W2060017723 cites W2132068224 @default.
• W2060017723 cites W2399490670 @default.
• W2060017723 cites W2571930439 @default.
• W2060017723 cites W57615732 @default.
• W2060017723 doi "https://doi.org/10.1111/j.1432-1033.1981.tb06176.x" @default.
• W2060017723 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7011805" @default.
• W2060017723 hasPublicationYear "2005" @default.
• W2060017723 type Work @default.
• W2060017723 sameAs 2060017723 @default.
• W2060017723 citedByCount "13" @default.
• W2060017723 crossrefType "journal-article" @default.
• W2060017723 hasAuthorship W2060017723A5032033695 @default.
• W2060017723 hasAuthorship W2060017723A5045358445 @default.
• W2060017723 hasAuthorship W2060017723A5048149002 @default.
• W2060017723 hasAuthorship W2060017723A5085379313 @default.
• W2060017723 hasConcept C101660505 @default.
• W2060017723 hasConcept C104292427 @default.
• W2060017723 hasConcept C104317684 @default.
• W2060017723 hasConcept C116084860 @default.
• W2060017723 hasConcept C121332964 @default.
• W2060017723 hasConcept C148898269 @default.
• W2060017723 hasConcept C181199279 @default.
• W2060017723 hasConcept C185592680 @default.
• W2060017723 hasConcept C18903297 @default.
• W2060017723 hasConcept C193363816 @default.
• W2060017723 hasConcept C2777289219 @default.
• W2060017723 hasConcept C41183919 @default.
• W2060017723 hasConcept C55493867 @default.
• W2060017723 hasConcept C56856141 @default.
• W2060017723 hasConcept C62520636 @default.
• W2060017723 hasConcept C79115784 @default.
• W2060017723 hasConcept C7927669 @default.
• W2060017723 hasConcept C86803240 @default.
• W2060017723 hasConceptScore W2060017723C101660505 @default.
• W2060017723 hasConceptScore W2060017723C104292427 @default.
• W2060017723 hasConceptScore W2060017723C104317684 @default.
• W2060017723 hasConceptScore W2060017723C116084860 @default.
• W2060017723 hasConceptScore W2060017723C121332964 @default.
• W2060017723 hasConceptScore W2060017723C148898269 @default.
• W2060017723 hasConceptScore W2060017723C181199279 @default.
• W2060017723 hasConceptScore W2060017723C185592680 @default.
• W2060017723 hasConceptScore W2060017723C18903297 @default.
• W2060017723 hasConceptScore W2060017723C193363816 @default.
• W2060017723 hasConceptScore W2060017723C2777289219 @default.
• W2060017723 hasConceptScore W2060017723C41183919 @default.
• W2060017723 hasConceptScore W2060017723C55493867 @default.
• W2060017723 hasConceptScore W2060017723C56856141 @default.
• W2060017723 hasConceptScore W2060017723C62520636 @default.
• W2060017723 hasConceptScore W2060017723C79115784 @default.
• W2060017723 hasConceptScore W2060017723C7927669 @default.
• W2060017723 hasConceptScore W2060017723C86803240 @default.
• W2060017723 hasLocation W20600177231 @default.
• W2060017723 hasLocation W20600177232 @default.
• W2060017723 hasOpenAccess W2060017723 @default.
• W2060017723 hasPrimaryLocation W20600177231 @default.
• W2060017723 hasRelatedWork W1510195768 @default.
• W2060017723 hasRelatedWork W1521506765 @default.
• W2060017723 hasRelatedWork W1551283513 @default.
• W2060017723 hasRelatedWork W1649084527 @default.
• W2060017723 hasRelatedWork W2025788670 @default.
• W2060017723 hasRelatedWork W2141876431 @default.
• W2060017723 hasRelatedWork W2155086483 @default.
• W2060017723 hasRelatedWork W2317998381 @default.
• W2060017723 hasRelatedWork W308904022 @default.
• W2060017723 hasRelatedWork W4289956857 @default.
• W2060017723 isParatext "false" @default.
• W2060017723 isRetracted "false" @default.
• W2060017723 magId "2060017723" @default.

• next