Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060050419> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2060050419 endingPage "3777" @default.
- W2060050419 startingPage "3769" @default.
- W2060050419 abstract "A reinvestigation of the metal ion activation and specificity of CTP synthetase was begun in order to separate effects due to binding of free metal ions and binding of nucleotide-complexed ions. Apo-CTP synthetase was prepared by dialysis against 5 mM EDTA. Analysis of apo-enzyme by atomic absorption spectroscopy revealed that all bound metal ions could be removed. Thus, apo-enzyme contained no detectable amounts of Mg2+, Mn2+, Cu2+, Zn2+, Co2+, Ni2+, or Fe2+. The half-saturation value of Mg(2+)-dependent enzyme activation was approximately 2.6 mM at a total concentration of 2 mM nucleotides (ATP plus UTP). These data suggest that the enzyme requires more Mg2+ for full catalytic activity than required simply to complex the nucleotide substrates. Analysis of velocity versus [Mg2+]free demonstrated that activity depends on [Mg2+]free. The half-saturation values for [Mg2+]free were 660 and 280 microM in the NH4(+)- and glutamine-dependent assays, respectively. The half-saturation values for [Mn2+]free and [Co2+]free were approximately 2.6 and 3.8 microM in the NH4(+)-dependent assay and 2.8 and 4.7 microM in the glutamine-dependent assay. These results are consistent with the presence of a separate binding site for free metal ion on the enzyme. Over the range of 0.1-10 mM, neither Cu2+, Zn2+, Ni2+, nor Ca2+ activated the enzyme. Also, both Cu2+ and Zn2+ were effective inhibitors of CTP synthetase in the absence of dithiothreitol at concentrations < 50 microM. Inhibition by Zn2+ was reversed by EDTA, whereas inhibition by Cu2+ was not. In the presence of dithiothreitol, Zn2+, Co2+ and Ni2+ inhibited the enzyme at less than 200 microM metal ion.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W2060050419 created "2016-06-24" @default.
- W2060050419 creator A5015871198 @default.
- W2060050419 creator A5018328217 @default.
- W2060050419 date "1993-04-13" @default.
- W2060050419 modified "2023-10-16" @default.
- W2060050419 title "Characterization of metal ion activation and inhibition of CTP synthetase" @default.
- W2060050419 cites W1527200958 @default.
- W2060050419 cites W1551007985 @default.
- W2060050419 cites W1603607026 @default.
- W2060050419 cites W1968314325 @default.
- W2060050419 cites W1970218664 @default.
- W2060050419 cites W1970803380 @default.
- W2060050419 cites W1974104587 @default.
- W2060050419 cites W1990665726 @default.
- W2060050419 cites W2000559841 @default.
- W2060050419 cites W2007665536 @default.
- W2060050419 cites W2021116136 @default.
- W2060050419 cites W2029470282 @default.
- W2060050419 cites W2044493879 @default.
- W2060050419 cites W2053851913 @default.
- W2060050419 cites W2061317980 @default.
- W2060050419 cites W2072897890 @default.
- W2060050419 cites W2085111510 @default.
- W2060050419 cites W2091969259 @default.
- W2060050419 cites W2129995310 @default.
- W2060050419 cites W2206940393 @default.
- W2060050419 cites W2208268550 @default.
- W2060050419 cites W2288211602 @default.
- W2060050419 cites W2410248087 @default.
- W2060050419 cites W68137331 @default.
- W2060050419 doi "https://doi.org/10.1021/bi00065a032" @default.
- W2060050419 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8385490" @default.
- W2060050419 hasPublicationYear "1993" @default.
- W2060050419 type Work @default.
- W2060050419 sameAs 2060050419 @default.
- W2060050419 citedByCount "21" @default.
- W2060050419 countsByYear W20600504192012 @default.
- W2060050419 countsByYear W20600504192016 @default.
- W2060050419 countsByYear W20600504192018 @default.
- W2060050419 countsByYear W20600504192022 @default.
- W2060050419 countsByYear W20600504192023 @default.
- W2060050419 crossrefType "journal-article" @default.
- W2060050419 hasAuthorship W2060050419A5015871198 @default.
- W2060050419 hasAuthorship W2060050419A5018328217 @default.
- W2060050419 hasConcept C104317684 @default.
- W2060050419 hasConcept C178790620 @default.
- W2060050419 hasConcept C181199279 @default.
- W2060050419 hasConcept C185592680 @default.
- W2060050419 hasConcept C199164860 @default.
- W2060050419 hasConcept C2777824588 @default.
- W2060050419 hasConcept C2779289688 @default.
- W2060050419 hasConcept C2779349466 @default.
- W2060050419 hasConcept C28212737 @default.
- W2060050419 hasConcept C512185932 @default.
- W2060050419 hasConcept C515207424 @default.
- W2060050419 hasConcept C544153396 @default.
- W2060050419 hasConcept C55493867 @default.
- W2060050419 hasConcept C71240020 @default.
- W2060050419 hasConceptScore W2060050419C104317684 @default.
- W2060050419 hasConceptScore W2060050419C178790620 @default.
- W2060050419 hasConceptScore W2060050419C181199279 @default.
- W2060050419 hasConceptScore W2060050419C185592680 @default.
- W2060050419 hasConceptScore W2060050419C199164860 @default.
- W2060050419 hasConceptScore W2060050419C2777824588 @default.
- W2060050419 hasConceptScore W2060050419C2779289688 @default.
- W2060050419 hasConceptScore W2060050419C2779349466 @default.
- W2060050419 hasConceptScore W2060050419C28212737 @default.
- W2060050419 hasConceptScore W2060050419C512185932 @default.
- W2060050419 hasConceptScore W2060050419C515207424 @default.
- W2060050419 hasConceptScore W2060050419C544153396 @default.
- W2060050419 hasConceptScore W2060050419C55493867 @default.
- W2060050419 hasConceptScore W2060050419C71240020 @default.
- W2060050419 hasIssue "14" @default.
- W2060050419 hasLocation W20600504191 @default.
- W2060050419 hasLocation W20600504192 @default.
- W2060050419 hasOpenAccess W2060050419 @default.
- W2060050419 hasPrimaryLocation W20600504191 @default.
- W2060050419 hasRelatedWork W1935703697 @default.
- W2060050419 hasRelatedWork W1981283490 @default.
- W2060050419 hasRelatedWork W1990585658 @default.
- W2060050419 hasRelatedWork W2016627433 @default.
- W2060050419 hasRelatedWork W2040461499 @default.
- W2060050419 hasRelatedWork W2085769706 @default.
- W2060050419 hasRelatedWork W2150080332 @default.
- W2060050419 hasRelatedWork W2357451662 @default.
- W2060050419 hasRelatedWork W2384149591 @default.
- W2060050419 hasRelatedWork W2404713397 @default.
- W2060050419 hasVolume "32" @default.
- W2060050419 isParatext "false" @default.
- W2060050419 isRetracted "false" @default.
- W2060050419 magId "2060050419" @default.
- W2060050419 workType "article" @default.