FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060083954> ?p ?o ?g. }

• W2060083954 endingPage "20230" @default.
• W2060083954 startingPage "20221" @default.
• W2060083954 abstract "Legionella pneumophila has been shown to secrete a protease termed major secretory protein (Msp). This protease belongs to the M4 family of metalloproteases and shares 62.9% sequence similarity with pseudolysin (EC 3.4.24.26). With the aim of developing a specific enzymatic assay for the detection and quantification of Msp, the Fluofast substrate library was screened using both enzymes in parallel. Moreover, based on the crystal structure of pseudolysin, a model of the Msp structure was built. Screening of the peptide library identified a lead substrate specifically cleaved by Msp that was subsequently optimized by rational design. The proposed model for Msp is consistent with the enzymatic characteristics of the studied peptide substrates and provides new structural information useful for the characterization of the protease. This study leads to the identification of the first selective and high affinity substrate for Msp that is able to detect picomolar concentrations of the purified enzyme. The identified substrate could be useful for the development of a novel method for the rapid detection of Legionella." @default.
• W2060083954 created "2016-06-24" @default.
• W2060083954 creator A5003595001 @default.
• W2060083954 creator A5013132778 @default.
• W2060083954 creator A5017094117 @default.
• W2060083954 creator A5021316807 @default.
• W2060083954 creator A5060369838 @default.
• W2060083954 creator A5068784944 @default.
• W2060083954 creator A5070561198 @default.
• W2060083954 date "2012-06-01" @default.
• W2060083954 modified "2023-09-27" @default.
• W2060083954 title "Highly Sensitive Quenched Fluorescent Substrate of Legionella Major Secretory Protein (Msp) Based on Its Structural Analysis" @default.
• W2060083954 cites W1501405625 @default.
• W2060083954 cites W1773971809 @default.
• W2060083954 cites W1886928416 @default.
• W2060083954 cites W1916368487 @default.
• W2060083954 cites W1980045184 @default.
• W2060083954 cites W1985469117 @default.
• W2060083954 cites W2050456292 @default.
• W2060083954 cites W2052247856 @default.
• W2060083954 cites W2056812552 @default.
• W2060083954 cites W2065283382 @default.
• W2060083954 cites W2101130672 @default.
• W2060083954 cites W2106867797 @default.
• W2060083954 cites W2106882534 @default.
• W2060083954 cites W2125679181 @default.
• W2060083954 cites W2127237625 @default.
• W2060083954 cites W2138741791 @default.
• W2060083954 cites W2140547602 @default.
• W2060083954 cites W2151177230 @default.
• W2060083954 cites W2163998847 @default.
• W2060083954 cites W2165381351 @default.
• W2060083954 cites W4237766996 @default.
• W2060083954 doi "https://doi.org/10.1074/jbc.m111.334334" @default.
• W2060083954 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3370204" @default.
• W2060083954 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22528499" @default.
• W2060083954 hasPublicationYear "2012" @default.
• W2060083954 type Work @default.
• W2060083954 sameAs 2060083954 @default.
• W2060083954 citedByCount "2" @default.
• W2060083954 countsByYear W20600839542021 @default.
• W2060083954 countsByYear W20600839542023 @default.
• W2060083954 crossrefType "journal-article" @default.
• W2060083954 hasAuthorship W2060083954A5003595001 @default.
• W2060083954 hasAuthorship W2060083954A5013132778 @default.
• W2060083954 hasAuthorship W2060083954A5017094117 @default.
• W2060083954 hasAuthorship W2060083954A5021316807 @default.
• W2060083954 hasAuthorship W2060083954A5060369838 @default.
• W2060083954 hasAuthorship W2060083954A5068784944 @default.
• W2060083954 hasAuthorship W2060083954A5070561198 @default.
• W2060083954 hasBestOaLocation W20600839542 @default.
• W2060083954 hasConcept C156399914 @default.
• W2060083954 hasConcept C181199279 @default.
• W2060083954 hasConcept C185592680 @default.
• W2060083954 hasConcept C18903297 @default.
• W2060083954 hasConcept C2776118409 @default.
• W2060083954 hasConcept C2776714187 @default.
• W2060083954 hasConcept C2777289219 @default.
• W2060083954 hasConcept C2779164008 @default.
• W2060083954 hasConcept C2779281246 @default.
• W2060083954 hasConcept C49039625 @default.
• W2060083954 hasConcept C523546767 @default.
• W2060083954 hasConcept C54355233 @default.
• W2060083954 hasConcept C55493867 @default.
• W2060083954 hasConcept C86803240 @default.
• W2060083954 hasConceptScore W2060083954C156399914 @default.
• W2060083954 hasConceptScore W2060083954C181199279 @default.
• W2060083954 hasConceptScore W2060083954C185592680 @default.
• W2060083954 hasConceptScore W2060083954C18903297 @default.
• W2060083954 hasConceptScore W2060083954C2776118409 @default.
• W2060083954 hasConceptScore W2060083954C2776714187 @default.
• W2060083954 hasConceptScore W2060083954C2777289219 @default.
• W2060083954 hasConceptScore W2060083954C2779164008 @default.
• W2060083954 hasConceptScore W2060083954C2779281246 @default.
• W2060083954 hasConceptScore W2060083954C49039625 @default.
• W2060083954 hasConceptScore W2060083954C523546767 @default.
• W2060083954 hasConceptScore W2060083954C54355233 @default.
• W2060083954 hasConceptScore W2060083954C55493867 @default.
• W2060083954 hasConceptScore W2060083954C86803240 @default.
• W2060083954 hasIssue "24" @default.
• W2060083954 hasLocation W20600839541 @default.
• W2060083954 hasLocation W20600839542 @default.
• W2060083954 hasLocation W20600839543 @default.
• W2060083954 hasLocation W20600839544 @default.
• W2060083954 hasOpenAccess W2060083954 @default.
• W2060083954 hasPrimaryLocation W20600839541 @default.
• W2060083954 hasRelatedWork W1037319764 @default.
• W2060083954 hasRelatedWork W1174660814 @default.
• W2060083954 hasRelatedWork W1915907597 @default.
• W2060083954 hasRelatedWork W1987020986 @default.
• W2060083954 hasRelatedWork W2060083954 @default.
• W2060083954 hasRelatedWork W2080747272 @default.
• W2060083954 hasRelatedWork W2085753387 @default.
• W2060083954 hasRelatedWork W2765553062 @default.
• W2060083954 hasRelatedWork W2780593008 @default.
• W2060083954 hasRelatedWork W921125394 @default.
• W2060083954 hasVolume "287" @default.
• W2060083954 isParatext "false" @default.

• next