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- W2060181898 abstract "A microassay for p34cdc2 based on the high affinity association between cdc2 and Schizosaccharomyces pombe p13suc1 has been developed. p13 purified from Escherichia coli was immobilized on microtiter plates and cellular lysate was incubated in the wells to allow the binding of cdc2 and its associated proteins. p34cdc2 was assayed either as a histone kinase or by immunological methods. The method was optimized for S. pombe cell extracts but can also be applied to other organisms such as Xenopus oocytes or HeLa cells. This rapid assay allows the specific determination of p34cdc2 histone H1 kinase activity in a very large number of samples." @default.
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- W2060181898 date "1990-05-01" @default.
- W2060181898 modified "2023-09-27" @default.
- W2060181898 title "A versatile microtiter assay for the universal cdc2 cell cycle regulator" @default.
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- W2060181898 doi "https://doi.org/10.1016/0003-2697(90)90422-6" @default.
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