FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060186677> ?p ?o ?g. }

• W2060186677 endingPage "108" @default.
• W2060186677 startingPage "103" @default.
• W2060186677 abstract "A novel system is described for mild elution of fusion proteins by competitive elution. The approach is based on displacement of immobilized fusions containing a monovalent IgG-binding staphylococcal protein A fragment (Z) from an IgG-affinity matrix by a divalent fragment fused to a serum-albumin-binding region derived from streptococcal protein G. Using real-time interaction analysis, the binding (Kaff) to polyclonal human IgG was found to be 3.3 (±0.4)×108M-1 for divalent ZZ and 2.0 (±0.1)×107M-1 for monovalent Z. This more than tenfold difference in binding strength ensures a high efficiency in the elution step. The competitor protein can specifically be removed and recovered from the elution mixture by subsequent passage through a human serum albumin(HSA)-affinity column, leaving only the target fusion protein in the flow-through fraction. Here, we show that a recombinant Klenow fragment of DNA polymerase I expressed in Escherichia coli can be recovered with high yield, and retained activity, from a crude bacterial lysate by IgG-affinity chromatography using mild conditions during both binding and elution." @default.
• W2060186677 created "2016-06-24" @default.
• W2060186677 creator A5002431291 @default.
• W2060186677 creator A5034785132 @default.
• W2060186677 creator A5058880814 @default.
• W2060186677 creator A5060045564 @default.
• W2060186677 creator A5060241561 @default.
• W2060186677 creator A5084408140 @default.
• W2060186677 date "1994-08-01" @default.
• W2060186677 modified "2023-10-15" @default.
• W2060186677 title "Competitive Elution of Protein A Fusion Proteins Allows Specific Recovery Under Mild Conditions" @default.
• W2060186677 cites W150462389 @default.
• W2060186677 cites W1593554557 @default.
• W2060186677 cites W1973214902 @default.
• W2060186677 cites W1975773001 @default.
• W2060186677 cites W1979927412 @default.
• W2060186677 cites W1987461205 @default.
• W2060186677 cites W1991516961 @default.
• W2060186677 cites W1993870692 @default.
• W2060186677 cites W1998511748 @default.
• W2060186677 cites W2005537634 @default.
• W2060186677 cites W2012604733 @default.
• W2060186677 cites W2028622989 @default.
• W2060186677 cites W2033556541 @default.
• W2060186677 cites W2035609763 @default.
• W2060186677 cites W2040249970 @default.
• W2060186677 cites W2040668246 @default.
• W2060186677 cites W2042997515 @default.
• W2060186677 cites W2055247303 @default.
• W2060186677 cites W2055454792 @default.
• W2060186677 cites W2065471902 @default.
• W2060186677 cites W2066089897 @default.
• W2060186677 cites W2068446924 @default.
• W2060186677 cites W2074969682 @default.
• W2060186677 cites W2081061541 @default.
• W2060186677 cites W2083269271 @default.
• W2060186677 cites W2100837269 @default.
• W2060186677 cites W2131626832 @default.
• W2060186677 cites W2165449806 @default.
• W2060186677 cites W2228598834 @default.
• W2060186677 cites W2402266146 @default.
• W2060186677 doi "https://doi.org/10.1111/j.1432-1033.1994.tb20000.x" @default.
• W2060186677 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8076629" @default.
• W2060186677 hasPublicationYear "1994" @default.
• W2060186677 type Work @default.
• W2060186677 sameAs 2060186677 @default.
• W2060186677 citedByCount "50" @default.
• W2060186677 countsByYear W20601866772012 @default.
• W2060186677 countsByYear W20601866772013 @default.
• W2060186677 countsByYear W20601866772014 @default.
• W2060186677 countsByYear W20601866772015 @default.
• W2060186677 countsByYear W20601866772016 @default.
• W2060186677 countsByYear W20601866772017 @default.
• W2060186677 countsByYear W20601866772023 @default.
• W2060186677 crossrefType "journal-article" @default.
• W2060186677 hasAuthorship W2060186677A5002431291 @default.
• W2060186677 hasAuthorship W2060186677A5034785132 @default.
• W2060186677 hasAuthorship W2060186677A5058880814 @default.
• W2060186677 hasAuthorship W2060186677A5060045564 @default.
• W2060186677 hasAuthorship W2060186677A5060241561 @default.
• W2060186677 hasAuthorship W2060186677A5084408140 @default.
• W2060186677 hasConcept C104317684 @default.
• W2060186677 hasConcept C123894998 @default.
• W2060186677 hasConcept C159654299 @default.
• W2060186677 hasConcept C178790620 @default.
• W2060186677 hasConcept C179247698 @default.
• W2060186677 hasConcept C181199279 @default.
• W2060186677 hasConcept C185592680 @default.
• W2060186677 hasConcept C203014093 @default.
• W2060186677 hasConcept C2777501222 @default.
• W2060186677 hasConcept C2779000548 @default.
• W2060186677 hasConcept C40767141 @default.
• W2060186677 hasConcept C43617362 @default.
• W2060186677 hasConcept C54415193 @default.
• W2060186677 hasConcept C552990157 @default.
• W2060186677 hasConcept C55493867 @default.
• W2060186677 hasConcept C57409179 @default.
• W2060186677 hasConcept C73826308 @default.
• W2060186677 hasConcept C74884574 @default.
• W2060186677 hasConcept C82381507 @default.
• W2060186677 hasConcept C86803240 @default.
• W2060186677 hasConcept C956191 @default.
• W2060186677 hasConceptScore W2060186677C104317684 @default.
• W2060186677 hasConceptScore W2060186677C123894998 @default.
• W2060186677 hasConceptScore W2060186677C159654299 @default.
• W2060186677 hasConceptScore W2060186677C178790620 @default.
• W2060186677 hasConceptScore W2060186677C179247698 @default.
• W2060186677 hasConceptScore W2060186677C181199279 @default.
• W2060186677 hasConceptScore W2060186677C185592680 @default.
• W2060186677 hasConceptScore W2060186677C203014093 @default.
• W2060186677 hasConceptScore W2060186677C2777501222 @default.
• W2060186677 hasConceptScore W2060186677C2779000548 @default.
• W2060186677 hasConceptScore W2060186677C40767141 @default.
• W2060186677 hasConceptScore W2060186677C43617362 @default.
• W2060186677 hasConceptScore W2060186677C54415193 @default.
• W2060186677 hasConceptScore W2060186677C552990157 @default.
• W2060186677 hasConceptScore W2060186677C55493867 @default.
• W2060186677 hasConceptScore W2060186677C57409179 @default.

• next