FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060194734> ?p ?o ?g. }

• W2060194734 endingPage "4317" @default.
• W2060194734 startingPage "4308" @default.
• W2060194734 abstract "Coronaviruses are responsible for a significant proportion of annual respiratory and enteric infections in humans and other mammals. The most prominent of these viruses is the severe acute respiratory syndrome coronavirus (SARS-CoV) which causes acute respiratory and gastrointestinal infection in humans. The coronavirus main protease, 3CLpro, is a key target for broad-spectrum antiviral development because of its critical role in viral maturation and high degree of structural conservation among coronaviruses. Dimerization is an indispensable requirement for the function of SARS 3CLpro and is regulated through mechanisms involving both direct and long-range interactions in the enzyme. While many of the binding interactions at the dimerization interface have been extensively studied, those that are important for long-range control are not well-understood. Characterization of these dimerization mechanisms is important for the structure-based design of new treatments targeting coronavirus-based infections. Here we report that Asn28, a residue 11 Å from the closest residue in the opposing monomer, is essential for the enzymatic activity and dimerization of SARS 3CLpro. Mutation of this residue to alanine almost completely inactivates the enzyme and results in a 19.2-fold decrease in the dimerization Kd. The crystallographic structure of the N28A mutant determined at 2.35 Å resolution reveals the critical role of Asn28 in maintaining the structural integrity of the active site and in orienting key residues involved in binding at the dimer interface and substrate catalysis. These findings provide deeper insight into complex mechanisms regulating the activity and dimerization of SARS 3CLpro." @default.
• W2060194734 created "2016-06-24" @default.
• W2060194734 creator A5006684849 @default.
• W2060194734 creator A5007948012 @default.
• W2060194734 creator A5029031647 @default.
• W2060194734 creator A5029989649 @default.
• W2060194734 creator A5067641191 @default.
• W2060194734 date "2010-05-03" @default.
• W2060194734 modified "2023-10-01" @default.
• W2060194734 title "Mutation of Asn28 Disrupts the Dimerization and Enzymatic Activity of SARS 3CL^pro," @default.
• W2060194734 cites W1539796472 @default.
• W2060194734 cites W1965411625 @default.
• W2060194734 cites W1965965133 @default.
• W2060194734 cites W1966480833 @default.
• W2060194734 cites W1975317507 @default.
• W2060194734 cites W1986191025 @default.
• W2060194734 cites W1987036857 @default.
• W2060194734 cites W2005098407 @default.
• W2060194734 cites W2010082441 @default.
• W2060194734 cites W2013083986 @default.
• W2060194734 cites W2016036730 @default.
• W2060194734 cites W2017178787 @default.
• W2060194734 cites W2021587345 @default.
• W2060194734 cites W2023981044 @default.
• W2060194734 cites W2025170735 @default.
• W2060194734 cites W2032660941 @default.
• W2060194734 cites W2036572208 @default.
• W2060194734 cites W2039466442 @default.
• W2060194734 cites W2039776546 @default.
• W2060194734 cites W2044733286 @default.
• W2060194734 cites W2055983838 @default.
• W2060194734 cites W2075379119 @default.
• W2060194734 cites W2080476827 @default.
• W2060194734 cites W2081912151 @default.
• W2060194734 cites W2084874437 @default.
• W2060194734 cites W2086407132 @default.
• W2060194734 cites W2103854602 @default.
• W2060194734 cites W2103869681 @default.
• W2060194734 cites W2106882534 @default.
• W2060194734 cites W2108018232 @default.
• W2060194734 cites W2125223017 @default.
• W2060194734 cites W2125976303 @default.
• W2060194734 cites W2127011149 @default.
• W2060194734 cites W2132260239 @default.
• W2060194734 cites W2132629607 @default.
• W2060194734 cites W2134061616 @default.
• W2060194734 cites W2144927470 @default.
• W2060194734 cites W2145693406 @default.
• W2060194734 cites W2146886475 @default.
• W2060194734 cites W2149696442 @default.
• W2060194734 cites W2153849867 @default.
• W2060194734 cites W2158812536 @default.
• W2060194734 cites W2164526213 @default.
• W2060194734 cites W2168247623 @default.
• W2060194734 cites W2168446943 @default.
• W2060194734 cites W2170933940 @default.
• W2060194734 cites W2171291163 @default.
• W2060194734 doi "https://doi.org/10.1021/bi1002585" @default.
• W2060194734 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2877132" @default.
• W2060194734 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20420403" @default.
• W2060194734 hasPublicationYear "2010" @default.
• W2060194734 type Work @default.
• W2060194734 sameAs 2060194734 @default.
• W2060194734 citedByCount "46" @default.
• W2060194734 countsByYear W20601947342012 @default.
• W2060194734 countsByYear W20601947342013 @default.
• W2060194734 countsByYear W20601947342014 @default.
• W2060194734 countsByYear W20601947342015 @default.
• W2060194734 countsByYear W20601947342016 @default.
• W2060194734 countsByYear W20601947342017 @default.
• W2060194734 countsByYear W20601947342019 @default.
• W2060194734 countsByYear W20601947342020 @default.
• W2060194734 countsByYear W20601947342021 @default.
• W2060194734 countsByYear W20601947342022 @default.
• W2060194734 countsByYear W20601947342023 @default.
• W2060194734 crossrefType "journal-article" @default.
• W2060194734 hasAuthorship W2060194734A5006684849 @default.
• W2060194734 hasAuthorship W2060194734A5007948012 @default.
• W2060194734 hasAuthorship W2060194734A5029031647 @default.
• W2060194734 hasAuthorship W2060194734A5029989649 @default.
• W2060194734 hasAuthorship W2060194734A5067641191 @default.
• W2060194734 hasBestOaLocation W20601947342 @default.
• W2060194734 hasConcept C104317684 @default.
• W2060194734 hasConcept C142724271 @default.
• W2060194734 hasConcept C143065580 @default.
• W2060194734 hasConcept C178790620 @default.
• W2060194734 hasConcept C181199279 @default.
• W2060194734 hasConcept C185592680 @default.
• W2060194734 hasConcept C2776714187 @default.
• W2060194734 hasConcept C2776844884 @default.
• W2060194734 hasConcept C2777648638 @default.
• W2060194734 hasConcept C2779134260 @default.
• W2060194734 hasConcept C2779546866 @default.
• W2060194734 hasConcept C2779856020 @default.
• W2060194734 hasConcept C2781338088 @default.
• W2060194734 hasConcept C3008058167 @default.
• W2060194734 hasConcept C41183919 @default.
• W2060194734 hasConcept C47701112 @default.

• next