Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060208608> ?p ?o ?g. }
- W2060208608 endingPage "3990" @default.
- W2060208608 startingPage "3979" @default.
- W2060208608 abstract "One pathway in forming synaptic-like microvesicles (SLMV) involves direct budding from the plasma membrane, requires adaptor protein 2 (AP2) and is brefeldin A (BFA) resistant. A second route leads from the plasma membrane to an endosomal intermediate from which SLMV bud in a BFA-sensitive, AP3-dependent manner. Because AP3 has been shown to bind to a di-leucine targeting signal in vitro, we have investigated whether this major class of targeting signals is capable of directing protein traffic to SLMV in vivo. We have found that a di-leucine signal within the cytoplasmic tail of human tyrosinase is responsible for the majority of the targeting of HRP-tyrosinase chimeras to SLMV in PC12 cells. Furthermore, we have discovered that a Met-Leu di-hydrophobic motif within the extreme C terminus of synaptotagmin I supports 20% of the SLMV targeting of a CD4-synaptotagmin chimera. All of the traffic to the SLMV mediated by either di-Leu or Met-Leu is BFA sensitive, strongly suggesting a role for AP3 and possibly for an endosomal intermediate in this process. The differential reduction in SLMV targeting for HRP-tyrosinase and CD4-synaptotagmin chimeras by di-alanine substitutions or BFA treatment implies that different proteins use the two routes to the SLMV to differing extents." @default.
- W2060208608 created "2016-06-24" @default.
- W2060208608 creator A5035690049 @default.
- W2060208608 creator A5072558369 @default.
- W2060208608 creator A5086757918 @default.
- W2060208608 date "1999-11-01" @default.
- W2060208608 modified "2023-10-01" @default.
- W2060208608 title "Di-Leucine Signals Mediate Targeting of Tyrosinase and Synaptotagmin to Synaptic-like Microvesicles within PC12 Cells" @default.
- W2060208608 cites W1483463937 @default.
- W2060208608 cites W1524901037 @default.
- W2060208608 cites W1537719134 @default.
- W2060208608 cites W1548736406 @default.
- W2060208608 cites W1591133722 @default.
- W2060208608 cites W1644644959 @default.
- W2060208608 cites W1698625455 @default.
- W2060208608 cites W1869532508 @default.
- W2060208608 cites W1963980287 @default.
- W2060208608 cites W1967817609 @default.
- W2060208608 cites W1968240918 @default.
- W2060208608 cites W1969722889 @default.
- W2060208608 cites W1970930136 @default.
- W2060208608 cites W1973583404 @default.
- W2060208608 cites W1977444829 @default.
- W2060208608 cites W1980548298 @default.
- W2060208608 cites W1982630258 @default.
- W2060208608 cites W1987281935 @default.
- W2060208608 cites W1989333387 @default.
- W2060208608 cites W1993446288 @default.
- W2060208608 cites W1994849653 @default.
- W2060208608 cites W1996862455 @default.
- W2060208608 cites W1999382407 @default.
- W2060208608 cites W2003560862 @default.
- W2060208608 cites W2005022545 @default.
- W2060208608 cites W2009008715 @default.
- W2060208608 cites W2011230954 @default.
- W2060208608 cites W2014181207 @default.
- W2060208608 cites W2018757497 @default.
- W2060208608 cites W2019908099 @default.
- W2060208608 cites W2021059521 @default.
- W2060208608 cites W2025472785 @default.
- W2060208608 cites W2026590190 @default.
- W2060208608 cites W2030039293 @default.
- W2060208608 cites W2034167933 @default.
- W2060208608 cites W2035477123 @default.
- W2060208608 cites W2037717413 @default.
- W2060208608 cites W2038045605 @default.
- W2060208608 cites W2040996008 @default.
- W2060208608 cites W2042471874 @default.
- W2060208608 cites W2056865617 @default.
- W2060208608 cites W2059171611 @default.
- W2060208608 cites W2061017589 @default.
- W2060208608 cites W2063790250 @default.
- W2060208608 cites W2064069829 @default.
- W2060208608 cites W2076364749 @default.
- W2060208608 cites W2077606430 @default.
- W2060208608 cites W2079435546 @default.
- W2060208608 cites W2082312089 @default.
- W2060208608 cites W2085340755 @default.
- W2060208608 cites W2086036400 @default.
- W2060208608 cites W2105417881 @default.
- W2060208608 cites W2119123632 @default.
- W2060208608 cites W2121357228 @default.
- W2060208608 cites W2125742253 @default.
- W2060208608 cites W213218024 @default.
- W2060208608 cites W2133530629 @default.
- W2060208608 cites W2133927394 @default.
- W2060208608 cites W2135319033 @default.
- W2060208608 cites W2136764202 @default.
- W2060208608 cites W2139930780 @default.
- W2060208608 cites W2140983793 @default.
- W2060208608 cites W2148188096 @default.
- W2060208608 cites W2152558091 @default.
- W2060208608 cites W2159058105 @default.
- W2060208608 cites W2165435442 @default.
- W2060208608 cites W2170611990 @default.
- W2060208608 cites W2172226184 @default.
- W2060208608 cites W2178812731 @default.
- W2060208608 cites W2401100074 @default.
- W2060208608 cites W637806715 @default.
- W2060208608 cites W75521371 @default.
- W2060208608 doi "https://doi.org/10.1091/mbc.10.11.3979" @default.
- W2060208608 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/25693" @default.
- W2060208608 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10564285" @default.
- W2060208608 hasPublicationYear "1999" @default.
- W2060208608 type Work @default.
- W2060208608 sameAs 2060208608 @default.
- W2060208608 citedByCount "63" @default.
- W2060208608 countsByYear W20602086082012 @default.
- W2060208608 countsByYear W20602086082013 @default.
- W2060208608 countsByYear W20602086082015 @default.
- W2060208608 countsByYear W20602086082018 @default.
- W2060208608 countsByYear W20602086082019 @default.
- W2060208608 countsByYear W20602086082020 @default.
- W2060208608 crossrefType "journal-article" @default.
- W2060208608 hasAuthorship W2060208608A5035690049 @default.
- W2060208608 hasAuthorship W2060208608A5072558369 @default.
- W2060208608 hasAuthorship W2060208608A5086757918 @default.
- W2060208608 hasBestOaLocation W20602086082 @default.