Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060272004> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2060272004 endingPage "254" @default.
- W2060272004 startingPage "245" @default.
- W2060272004 abstract "Zinc-free bovine insulin has been studied by sedimentation equilibrium and circular dichroism. At pH 7.4, ionic strength 0.368, and a temperature of 20 °C, the peptide hormone appears to form a monomer-dimer-hexamer system characterized by a second virial coefficient of 8·10-6mol·dl·g-2, and association constants of 2.2·104 M-1 and 8.6·109 M-2 for monomer-dimer and dimer-hexamer formation, respectively. The dimerization free energy is —5.8 kcal/mol and the free energy for hexamer formation per (dimer) contact site is —4.4 kcal/mol. Guanidinium chloride induces marked changes in the self-association, the far ultraviolet circular dichroic spectrum, and the near ultraviolet circular dichroic spectrum. In the presence of 1.5 M guanidinium chloride Zn2+-free insulin exists mainly as a monomer-dimer system, and above 3.5 M guanidinium chloride the hormone forms a non-ideal monodisperse system. A broad transition, indicated by changes in the far ultraviolet circular dichroic spectrum between 2 and 6 M guanidinium chloride, probably results from a loss of secondary structure with increasing denaturant concentration. Under the conditions used, the near ultraviolet circular dichroic spectrum of native Zn2+-free insulin was not dependent on hormone concentration, although an apparent transition midpoint, measured as a decrease in the ellipticity at 270 nm with increasing guanidinium chloride concentration between 0 and 2 M, was dependent on insulin concentration. These results suggest that major changes in insulin secondary and tertiary structure occur over a range of denaturant concentrations in which insulin exists principally in the monomeric form. Thus, these data suggest that the maintenance of the insulin quarternary structure is not a prerequisite for the preservation of a considerable degree of secondary structure in the insulin molecule." @default.
- W2060272004 created "2016-06-24" @default.
- W2060272004 creator A5008929672 @default.
- W2060272004 creator A5058522048 @default.
- W2060272004 creator A5089145622 @default.
- W2060272004 date "1977-09-01" @default.
- W2060272004 modified "2023-10-16" @default.
- W2060272004 title "Conformational stability and self-association of zinc-free bovine insulin at neutral pH" @default.
- W2060272004 cites W1649140860 @default.
- W2060272004 cites W1949731655 @default.
- W2060272004 cites W1985959092 @default.
- W2060272004 cites W2000563495 @default.
- W2060272004 cites W2002194411 @default.
- W2060272004 cites W2006989237 @default.
- W2060272004 cites W2020975211 @default.
- W2060272004 cites W2046152615 @default.
- W2060272004 cites W2052149816 @default.
- W2060272004 cites W2064134818 @default.
- W2060272004 cites W2064968594 @default.
- W2060272004 cites W2077315899 @default.
- W2060272004 cites W2081110930 @default.
- W2060272004 cites W2084205559 @default.
- W2060272004 cites W2087395811 @default.
- W2060272004 cites W2114003924 @default.
- W2060272004 cites W2138090458 @default.
- W2060272004 cites W2171513964 @default.
- W2060272004 cites W2217579908 @default.
- W2060272004 cites W2315644272 @default.
- W2060272004 cites W3145404956 @default.
- W2060272004 cites W4211239749 @default.
- W2060272004 cites W4321429423 @default.
- W2060272004 cites W964503500 @default.
- W2060272004 doi "https://doi.org/10.1016/0005-2795(77)90152-0" @default.
- W2060272004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20156" @default.
- W2060272004 hasPublicationYear "1977" @default.
- W2060272004 type Work @default.
- W2060272004 sameAs 2060272004 @default.
- W2060272004 citedByCount "28" @default.
- W2060272004 countsByYear W20602720042018 @default.
- W2060272004 countsByYear W20602720042020 @default.
- W2060272004 crossrefType "journal-article" @default.
- W2060272004 hasAuthorship W2060272004A5008929672 @default.
- W2060272004 hasAuthorship W2060272004A5058522048 @default.
- W2060272004 hasAuthorship W2060272004A5089145622 @default.
- W2060272004 hasConcept C133571119 @default.
- W2060272004 hasConcept C166940927 @default.
- W2060272004 hasConcept C178790620 @default.
- W2060272004 hasConcept C181199279 @default.
- W2060272004 hasConcept C185592680 @default.
- W2060272004 hasConcept C189754071 @default.
- W2060272004 hasConcept C2777654166 @default.
- W2060272004 hasConcept C2779546866 @default.
- W2060272004 hasConcept C43617362 @default.
- W2060272004 hasConcept C46749446 @default.
- W2060272004 hasConcept C521977710 @default.
- W2060272004 hasConcept C54689828 @default.
- W2060272004 hasConcept C8010536 @default.
- W2060272004 hasConceptScore W2060272004C133571119 @default.
- W2060272004 hasConceptScore W2060272004C166940927 @default.
- W2060272004 hasConceptScore W2060272004C178790620 @default.
- W2060272004 hasConceptScore W2060272004C181199279 @default.
- W2060272004 hasConceptScore W2060272004C185592680 @default.
- W2060272004 hasConceptScore W2060272004C189754071 @default.
- W2060272004 hasConceptScore W2060272004C2777654166 @default.
- W2060272004 hasConceptScore W2060272004C2779546866 @default.
- W2060272004 hasConceptScore W2060272004C43617362 @default.
- W2060272004 hasConceptScore W2060272004C46749446 @default.
- W2060272004 hasConceptScore W2060272004C521977710 @default.
- W2060272004 hasConceptScore W2060272004C54689828 @default.
- W2060272004 hasConceptScore W2060272004C8010536 @default.
- W2060272004 hasIssue "1" @default.
- W2060272004 hasLocation W20602720041 @default.
- W2060272004 hasLocation W20602720042 @default.
- W2060272004 hasOpenAccess W2060272004 @default.
- W2060272004 hasPrimaryLocation W20602720041 @default.
- W2060272004 hasRelatedWork W195259517 @default.
- W2060272004 hasRelatedWork W1979908937 @default.
- W2060272004 hasRelatedWork W2004053657 @default.
- W2060272004 hasRelatedWork W2034223014 @default.
- W2060272004 hasRelatedWork W2040332722 @default.
- W2060272004 hasRelatedWork W2043843778 @default.
- W2060272004 hasRelatedWork W2060272004 @default.
- W2060272004 hasRelatedWork W2066467758 @default.
- W2060272004 hasRelatedWork W2097622231 @default.
- W2060272004 hasRelatedWork W349791440 @default.
- W2060272004 hasVolume "494" @default.
- W2060272004 isParatext "false" @default.
- W2060272004 isRetracted "false" @default.
- W2060272004 magId "2060272004" @default.
- W2060272004 workType "article" @default.