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- W2060280090 abstract "The Arabidopsis thaliana uncoupling protein (UCP) gene was expressed in Escherichia coli and isolated protein reconstituted into liposomes. Linoleic acid‐induced H + fluxes were sensitive to purine nucleotide inhibition with an apparent K i (in mM) of 0.8 (GDP), 0.85 (ATP), 0.98 (GTP), and 1.41 (ADP); the inhibition was pH‐dependent. Kinetics of At PUMP1‐mediated H + fluxes were determined for lauric, myristic, palmitic, oleic, linoleic, and linolenic acids. Properties of recombinant At PUMP1 indicate that it represents a plant counterpart of animal UCP2 or UCP3. This work brings the functional and genetic approaches together for the first time, providing strong support that At PUMP1 is truly an UCP." @default.
- W2060280090 created "2016-06-24" @default.
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- W2060280090 date "2001-08-28" @default.
- W2060280090 modified "2023-10-15" @default.
- W2060280090 title "Functional reconstitution of <i>Arabidopsis thaliana</i> plant uncoupling mitochondrial protein (<i>At</i>PUMP1) expressed in <i>Escherichia coli</i>" @default.
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- W2060280090 doi "https://doi.org/10.1016/s0014-5793(01)02835-6" @default.
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