Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060380231> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2060380231 endingPage "209a" @default.
- W2060380231 startingPage "209a" @default.
- W2060380231 abstract "Telomeres function to protect the end of linear chromosomes from damage and degradation. Human telomeres consists of several kilobases of the tandem repeat d(TTAGGG), which terminate in a 3′-single stranded overhang capable of forming G-Quadruplex structures. Telomeric G-Quadruplexes have potential roles in the maintenance of telomere integrity and the control of telomerase activity, an enzyme responsible for elongation of the telomeric overhang that is over expressed in a wide variety of cancers. Investigation of intramolecular G-Quadruplexes corresponding to the human telomere is complicated by the formation of multiple rapidly interconverting conformations. The multiple telomere G-Quadruplex conformations are identified by unique folding geometries containing distinct connecting loop regions.Time-resolved fluorescent measurements have been conducted to characterize the stability and heterogeneity of the telomeric G-Quadruplex forming sequence d(AGGG(TTAGGG)3). The fluorescent adenine analog, 2-aminopurine, served as an internal fluorescent probe of G-Quadruplex conformation and was used to specifically monitor loop regions. 2-aminopurine demonstrates a complex fluorescent decay upon incorporation into nucleic acid polymers that can be simplified by transformation of time-resolved data to an (S,G) phasor diagram. Phasor diagrams were used to monitor G-Quadruplex formation upon addition of NaCl or KCl and elucidated a unique KCl-dependent transition. The digestion of G-Quadruplex structure by a single-strand specific nuclease and acrylamide quenching are also evaluated through phasor diagrams. This work demonstrates the utility of phasor diagrams for the study of nucleic acid systems and the ability to sensitively monitor complex fluorescent decays that may complicate analysis through nonlinear regression techniques. Supported by awards RO1CA35635 and RO1GM077422 from the NCI and NIGMS." @default.
- W2060380231 created "2016-06-24" @default.
- W2060380231 creator A5004574073 @default.
- W2060380231 creator A5012431846 @default.
- W2060380231 creator A5035101442 @default.
- W2060380231 date "2012-01-01" @default.
- W2060380231 modified "2023-10-03" @default.
- W2060380231 title "Phasor Diagram Analysis of Telomeric G-Quadruplex Stability and Heterogeneity" @default.
- W2060380231 doi "https://doi.org/10.1016/j.bpj.2011.11.1140" @default.
- W2060380231 hasPublicationYear "2012" @default.
- W2060380231 type Work @default.
- W2060380231 sameAs 2060380231 @default.
- W2060380231 citedByCount "0" @default.
- W2060380231 crossrefType "journal-article" @default.
- W2060380231 hasAuthorship W2060380231A5004574073 @default.
- W2060380231 hasAuthorship W2060380231A5012431846 @default.
- W2060380231 hasAuthorship W2060380231A5035101442 @default.
- W2060380231 hasBestOaLocation W20603802311 @default.
- W2060380231 hasConcept C104317684 @default.
- W2060380231 hasConcept C121332964 @default.
- W2060380231 hasConcept C121745418 @default.
- W2060380231 hasConcept C12554922 @default.
- W2060380231 hasConcept C163258240 @default.
- W2060380231 hasConcept C176605952 @default.
- W2060380231 hasConcept C177336024 @default.
- W2060380231 hasConcept C185592680 @default.
- W2060380231 hasConcept C24107716 @default.
- W2060380231 hasConcept C2777271071 @default.
- W2060380231 hasConcept C552990157 @default.
- W2060380231 hasConcept C55493867 @default.
- W2060380231 hasConcept C62520636 @default.
- W2060380231 hasConcept C83762044 @default.
- W2060380231 hasConcept C86803240 @default.
- W2060380231 hasConcept C89227174 @default.
- W2060380231 hasConcept C91881484 @default.
- W2060380231 hasConcept C94581717 @default.
- W2060380231 hasConceptScore W2060380231C104317684 @default.
- W2060380231 hasConceptScore W2060380231C121332964 @default.
- W2060380231 hasConceptScore W2060380231C121745418 @default.
- W2060380231 hasConceptScore W2060380231C12554922 @default.
- W2060380231 hasConceptScore W2060380231C163258240 @default.
- W2060380231 hasConceptScore W2060380231C176605952 @default.
- W2060380231 hasConceptScore W2060380231C177336024 @default.
- W2060380231 hasConceptScore W2060380231C185592680 @default.
- W2060380231 hasConceptScore W2060380231C24107716 @default.
- W2060380231 hasConceptScore W2060380231C2777271071 @default.
- W2060380231 hasConceptScore W2060380231C552990157 @default.
- W2060380231 hasConceptScore W2060380231C55493867 @default.
- W2060380231 hasConceptScore W2060380231C62520636 @default.
- W2060380231 hasConceptScore W2060380231C83762044 @default.
- W2060380231 hasConceptScore W2060380231C86803240 @default.
- W2060380231 hasConceptScore W2060380231C89227174 @default.
- W2060380231 hasConceptScore W2060380231C91881484 @default.
- W2060380231 hasConceptScore W2060380231C94581717 @default.
- W2060380231 hasIssue "3" @default.
- W2060380231 hasLocation W20603802311 @default.
- W2060380231 hasOpenAccess W2060380231 @default.
- W2060380231 hasPrimaryLocation W20603802311 @default.
- W2060380231 hasRelatedWork W1938216854 @default.
- W2060380231 hasRelatedWork W1985690897 @default.
- W2060380231 hasRelatedWork W2009667051 @default.
- W2060380231 hasRelatedWork W2016668244 @default.
- W2060380231 hasRelatedWork W2028673140 @default.
- W2060380231 hasRelatedWork W2048215993 @default.
- W2060380231 hasRelatedWork W2072258154 @default.
- W2060380231 hasRelatedWork W2078024052 @default.
- W2060380231 hasRelatedWork W2103630737 @default.
- W2060380231 hasRelatedWork W2204099251 @default.
- W2060380231 hasRelatedWork W2224705212 @default.
- W2060380231 hasRelatedWork W2235942998 @default.
- W2060380231 hasRelatedWork W2270133955 @default.
- W2060380231 hasRelatedWork W2324217022 @default.
- W2060380231 hasRelatedWork W2417860154 @default.
- W2060380231 hasRelatedWork W2586894471 @default.
- W2060380231 hasRelatedWork W2969283119 @default.
- W2060380231 hasRelatedWork W3004726456 @default.
- W2060380231 hasRelatedWork W3181926755 @default.
- W2060380231 hasRelatedWork W3184503255 @default.
- W2060380231 hasVolume "102" @default.
- W2060380231 isParatext "false" @default.
- W2060380231 isRetracted "false" @default.
- W2060380231 magId "2060380231" @default.
- W2060380231 workType "article" @default.