FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060393137> ?p ?o ?g. }

• W2060393137 endingPage "883" @default.
• W2060393137 startingPage "875" @default.
• W2060393137 abstract "The third variable (V3) domain of HIV-1 gp120 envelope glycoprotein is critical for HIV-1 entry and represents an attractive target for vaccine design. There are three conserved N-glycans within or around the V3 loop. The N295 and N332 glycans at the base of V3 are usually characterized as high-mannose type in gp120, and the N301 glycan is a complex type. We report in this paper the expression and characterization of glycosylated, full-size V3 domain derived from HIV-1(Bal) strain as an IgG1-Fc fusion protein, including its binding to two broadly HIV-neutralizing antibodies 2G12 and 447-52D. It was found that expressing the V3-Fc fusion protein in the HEK293T cells resulted in the production of a glycoform in which all the N-glycans were complex type, in contrast to the glycosylation pattern of V3 in the context of gp120, where the N295 and N332 glycans are high-mannose type. Controlling the glycosylation to restore an epitope of antibody 2G12 was achieved by using an inhibitor of glycan processing enzymes. Mutational studies indicate that the glycan at N301 slightly decreases the binding of V3-Fc to antibody 447-52D, but it can significantly enhance the binding of the V3-Fc to antibody 2G12 when it is changed to a high-mannose type N-glycan. The high-mannose type V3-Fc fusion protein that includes both the 2G12 and 447-52D epitopes represents an interesting immunogen that may be able to raise anti-HIV neutralizing antibodies." @default.
• W2060393137 created "2016-06-24" @default.
• W2060393137 creator A5019540364 @default.
• W2060393137 creator A5023777560 @default.
• W2060393137 creator A5036627086 @default.
• W2060393137 creator A5077552203 @default.
• W2060393137 creator A5088737664 @default.
• W2060393137 date "2010-04-06" @default.
• W2060393137 modified "2023-10-06" @default.
• W2060393137 title "Expression, Glycoform Characterization, and Antibody-Binding of HIV-1 V3 Glycopeptide Domain Fused with Human IgG1-Fc" @default.
• W2060393137 cites W150399887 @default.
• W2060393137 cites W1505517942 @default.
• W2060393137 cites W1559766260 @default.
• W2060393137 cites W1794879226 @default.
• W2060393137 cites W1971158720 @default.
• W2060393137 cites W1982488181 @default.
• W2060393137 cites W1985711808 @default.
• W2060393137 cites W1987471547 @default.
• W2060393137 cites W1994322627 @default.
• W2060393137 cites W1996061504 @default.
• W2060393137 cites W1997902536 @default.
• W2060393137 cites W1998987175 @default.
• W2060393137 cites W2010521915 @default.
• W2060393137 cites W2024734109 @default.
• W2060393137 cites W2024977015 @default.
• W2060393137 cites W2033032481 @default.
• W2060393137 cites W2049544926 @default.
• W2060393137 cites W2050982497 @default.
• W2060393137 cites W2054547688 @default.
• W2060393137 cites W2056493803 @default.
• W2060393137 cites W2057457338 @default.
• W2060393137 cites W2067107443 @default.
• W2060393137 cites W2071914798 @default.
• W2060393137 cites W2076584494 @default.
• W2060393137 cites W2093282128 @default.
• W2060393137 cites W2099308199 @default.
• W2060393137 cites W2106183724 @default.
• W2060393137 cites W2109115694 @default.
• W2060393137 cites W2124445678 @default.
• W2060393137 cites W2131974188 @default.
• W2060393137 cites W2141444589 @default.
• W2060393137 cites W2145462968 @default.
• W2060393137 cites W2151344352 @default.
• W2060393137 cites W2163558461 @default.
• W2060393137 cites W2172063653 @default.
• W2060393137 doi "https://doi.org/10.1021/bc9004238" @default.
• W2060393137 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2901909" @default.
• W2060393137 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20369886" @default.
• W2060393137 hasPublicationYear "2010" @default.
• W2060393137 type Work @default.
• W2060393137 sameAs 2060393137 @default.
• W2060393137 citedByCount "17" @default.
• W2060393137 countsByYear W20603931372013 @default.
• W2060393137 countsByYear W20603931372014 @default.
• W2060393137 countsByYear W20603931372015 @default.
• W2060393137 countsByYear W20603931372016 @default.
• W2060393137 countsByYear W20603931372017 @default.
• W2060393137 countsByYear W20603931372021 @default.
• W2060393137 crossrefType "journal-article" @default.
• W2060393137 hasAuthorship W2060393137A5019540364 @default.
• W2060393137 hasAuthorship W2060393137A5023777560 @default.
• W2060393137 hasAuthorship W2060393137A5036627086 @default.
• W2060393137 hasAuthorship W2060393137A5077552203 @default.
• W2060393137 hasAuthorship W2060393137A5088737664 @default.
• W2060393137 hasBestOaLocation W20603931372 @default.
• W2060393137 hasConcept C104317684 @default.
• W2060393137 hasConcept C108625454 @default.
• W2060393137 hasConcept C153911025 @default.
• W2060393137 hasConcept C159654299 @default.
• W2060393137 hasConcept C167625842 @default.
• W2060393137 hasConcept C185592680 @default.
• W2060393137 hasConcept C195616568 @default.
• W2060393137 hasConcept C203014093 @default.
• W2060393137 hasConcept C206212055 @default.
• W2060393137 hasConcept C2777313579 @default.
• W2060393137 hasConcept C2779909307 @default.
• W2060393137 hasConcept C2779921545 @default.
• W2060393137 hasConcept C542903549 @default.
• W2060393137 hasConcept C55493867 @default.
• W2060393137 hasConcept C86803240 @default.
• W2060393137 hasConceptScore W2060393137C104317684 @default.
• W2060393137 hasConceptScore W2060393137C108625454 @default.
• W2060393137 hasConceptScore W2060393137C153911025 @default.
• W2060393137 hasConceptScore W2060393137C159654299 @default.
• W2060393137 hasConceptScore W2060393137C167625842 @default.
• W2060393137 hasConceptScore W2060393137C185592680 @default.
• W2060393137 hasConceptScore W2060393137C195616568 @default.
• W2060393137 hasConceptScore W2060393137C203014093 @default.
• W2060393137 hasConceptScore W2060393137C206212055 @default.
• W2060393137 hasConceptScore W2060393137C2777313579 @default.
• W2060393137 hasConceptScore W2060393137C2779909307 @default.
• W2060393137 hasConceptScore W2060393137C2779921545 @default.
• W2060393137 hasConceptScore W2060393137C542903549 @default.
• W2060393137 hasConceptScore W2060393137C55493867 @default.
• W2060393137 hasConceptScore W2060393137C86803240 @default.
• W2060393137 hasIssue "5" @default.
• W2060393137 hasLocation W20603931371 @default.
• W2060393137 hasLocation W20603931372 @default.

• next