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• W2060403781 abstract "A gene encoding a type I topoisomerase (TOP1) was isolated from Candida albicans, sequenced, and expressed in Saccharomyces cerevisiae. The TOP1 gene was identified from a C. albicans genomic library by hybridization with the product of a polymerase chain reaction with degenerate primer sets encoding regions conserved in other TOP1 genes. A clone containing an open reading frame of 2463 bp and predicted to encode a protein of 778 amino acids with sequence similarity to eukaryotic type I topoisomerases was identified. The C. albicans TOP1 gene restored camptothecin sensitivity and increased the topoisomerase activity in S. cerevisiae, indicating that the DNA fragment encodes a functional C. albicans topoisomerase I." @default.
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• W2060403781 date "1996-05-01" @default.
• W2060403781 modified "2023-10-01" @default.
• W2060403781 title "Identification of the gene encoding DNA topoisomerase I from<i>Candida albicans</i>" @default.
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• W2060403781 doi "https://doi.org/10.1111/j.1574-6968.1996.tb08143.x" @default.
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