Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060480019> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2060480019 endingPage "92" @default.
- W2060480019 startingPage "85" @default.
- W2060480019 abstract "A standardized enzyme-linked immunosorbent assay (ELISA) was used to examine the capacity of immunoassay plates to prevent non-specific protein binding under blocking conditions. Data from 16 types of 96-well microtitre plate from seven commercial sources, are described. Plates were evaluated with respect to their capacity to adsorb a conjugated antibody in diluent buffer containing non-ionic detergent Tween 20 (0.05%) and skimmed milk proteins (5%). Plates with an absorbance value of ≥0.05, in not more than one well, were defined as within acceptable limits. Major problems were seen in high binding γ-irradiated polystyrene plates, from all sources, where only ≤30% of plates were acceptable. These showed high, randomly distributed, non-specific binding, with some wells showing absorbance values >2.0. Similar results were obtained when high binding plates were repeatedly γ-irradiated, and after γ-irradiation of low binding polystyrene plates. For high binding, non-γ-irradiated polystyrene plates, approximately 70% of plates were acceptable. Better results (86–100% acceptability) were observed for all low binding polystyrene plates. Only one source in three provided acceptable, low binding, polyvinylchloride plates. This paper confirms a widely held view that non-specific binding to certain plates could be a serious factor in both the development and application of ELISAs. Therefore, the test protocol described is proposed as an additional quality control method for certifying ELISA plates by commercial companies." @default.
- W2060480019 created "2016-06-24" @default.
- W2060480019 creator A5002841490 @default.
- W2060480019 creator A5003464863 @default.
- W2060480019 creator A5043026997 @default.
- W2060480019 creator A5043702513 @default.
- W2060480019 creator A5054582278 @default.
- W2060480019 creator A5083374834 @default.
- W2060480019 creator A5084621279 @default.
- W2060480019 date "1999-06-01" @default.
- W2060480019 modified "2023-09-27" @default.
- W2060480019 title "Identification of unacceptable background caused by non-specific protein adsorption to the plastic surface of 96-well immunoassay plates using a standardized enzyme-linked immunosorbent assay procedure" @default.
- W2060480019 cites W2032594229 @default.
- W2060480019 cites W2084855813 @default.
- W2060480019 doi "https://doi.org/10.1016/s0022-1759(99)00051-4" @default.
- W2060480019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10410974" @default.
- W2060480019 hasPublicationYear "1999" @default.
- W2060480019 type Work @default.
- W2060480019 sameAs 2060480019 @default.
- W2060480019 citedByCount "49" @default.
- W2060480019 countsByYear W20604800192012 @default.
- W2060480019 countsByYear W20604800192013 @default.
- W2060480019 countsByYear W20604800192014 @default.
- W2060480019 countsByYear W20604800192015 @default.
- W2060480019 countsByYear W20604800192016 @default.
- W2060480019 countsByYear W20604800192017 @default.
- W2060480019 countsByYear W20604800192020 @default.
- W2060480019 countsByYear W20604800192023 @default.
- W2060480019 crossrefType "journal-article" @default.
- W2060480019 hasAuthorship W2060480019A5002841490 @default.
- W2060480019 hasAuthorship W2060480019A5003464863 @default.
- W2060480019 hasAuthorship W2060480019A5043026997 @default.
- W2060480019 hasAuthorship W2060480019A5043702513 @default.
- W2060480019 hasAuthorship W2060480019A5054582278 @default.
- W2060480019 hasAuthorship W2060480019A5083374834 @default.
- W2060480019 hasAuthorship W2060480019A5084621279 @default.
- W2060480019 hasConcept C121332964 @default.
- W2060480019 hasConcept C13965031 @default.
- W2060480019 hasConcept C150394285 @default.
- W2060480019 hasConcept C159654299 @default.
- W2060480019 hasConcept C175804951 @default.
- W2060480019 hasConcept C178790620 @default.
- W2060480019 hasConcept C184651966 @default.
- W2060480019 hasConcept C185592680 @default.
- W2060480019 hasConcept C187428577 @default.
- W2060480019 hasConcept C203014093 @default.
- W2060480019 hasConcept C2777922577 @default.
- W2060480019 hasConcept C2778861558 @default.
- W2060480019 hasConcept C2780420688 @default.
- W2060480019 hasConcept C43617362 @default.
- W2060480019 hasConcept C521977710 @default.
- W2060480019 hasConcept C62520636 @default.
- W2060480019 hasConcept C86803240 @default.
- W2060480019 hasConcept C91881484 @default.
- W2060480019 hasConcept C98015330 @default.
- W2060480019 hasConceptScore W2060480019C121332964 @default.
- W2060480019 hasConceptScore W2060480019C13965031 @default.
- W2060480019 hasConceptScore W2060480019C150394285 @default.
- W2060480019 hasConceptScore W2060480019C159654299 @default.
- W2060480019 hasConceptScore W2060480019C175804951 @default.
- W2060480019 hasConceptScore W2060480019C178790620 @default.
- W2060480019 hasConceptScore W2060480019C184651966 @default.
- W2060480019 hasConceptScore W2060480019C185592680 @default.
- W2060480019 hasConceptScore W2060480019C187428577 @default.
- W2060480019 hasConceptScore W2060480019C203014093 @default.
- W2060480019 hasConceptScore W2060480019C2777922577 @default.
- W2060480019 hasConceptScore W2060480019C2778861558 @default.
- W2060480019 hasConceptScore W2060480019C2780420688 @default.
- W2060480019 hasConceptScore W2060480019C43617362 @default.
- W2060480019 hasConceptScore W2060480019C521977710 @default.
- W2060480019 hasConceptScore W2060480019C62520636 @default.
- W2060480019 hasConceptScore W2060480019C86803240 @default.
- W2060480019 hasConceptScore W2060480019C91881484 @default.
- W2060480019 hasConceptScore W2060480019C98015330 @default.
- W2060480019 hasIssue "1-2" @default.
- W2060480019 hasLocation W20604800191 @default.
- W2060480019 hasLocation W20604800192 @default.
- W2060480019 hasOpenAccess W2060480019 @default.
- W2060480019 hasPrimaryLocation W20604800191 @default.
- W2060480019 hasRelatedWork W2014485428 @default.
- W2060480019 hasRelatedWork W2025124468 @default.
- W2060480019 hasRelatedWork W2026419386 @default.
- W2060480019 hasRelatedWork W2055294708 @default.
- W2060480019 hasRelatedWork W2075861635 @default.
- W2060480019 hasRelatedWork W2087052575 @default.
- W2060480019 hasRelatedWork W2115021388 @default.
- W2060480019 hasRelatedWork W2189090409 @default.
- W2060480019 hasRelatedWork W2359926239 @default.
- W2060480019 hasRelatedWork W2388259567 @default.
- W2060480019 hasVolume "226" @default.
- W2060480019 isParatext "false" @default.
- W2060480019 isRetracted "false" @default.
- W2060480019 magId "2060480019" @default.
- W2060480019 workType "article" @default.