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- W2060545093 abstract "Substantial evidence suggests that the aggregation of the presynaptic protein α-synuclein is a key step in the etiology of Parkinson's disease (PD). Although the molecular mechanisms underlying α-synuclein aggregation remain unknown, oxidative stress has been implicated in the pathogenesis of PD. Here, we report the effects of tyrosine nitration on the propensity of human recombinant α-synuclein to fibrillate in vitro. The properties of nitrated α-synuclein were investigated using a variety of biophysical and biochemical techniques, which revealed that nitration led to formation of a partially folded conformation with increased secondary structure relative to the intrinsically disordered structure of the monomer, and to oligomerization at neutral pH. The degree of self-association was concentration-dependent, but at 1 mg/mL, nitrated α-synuclein was predominantly an octamer. At low pH, small-angle X-ray scattering data indicated that the nitrated protein was monomeric. α-Synuclein fibrillation at neutral pH was completely inhibited by nitrotyrosination and is attributed to the formation of stable soluble oligomers. The presence of heparin or metals did not overcome the inhibition; however, the inhibitory effect was eliminated at low pH. The addition of nitrated α-synuclein inhibited fibrillation of non-modified α-synuclein at neutral pH. Potential implications of these findings to the etiology of Parkinson's disease are discussed." @default.
- W2060545093 created "2016-06-24" @default.
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- W2060545093 date "2005-03-01" @default.
- W2060545093 modified "2023-10-11" @default.
- W2060545093 title "Effects of nitration on the structure and aggregation of α-synuclein" @default.
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- W2060545093 doi "https://doi.org/10.1016/j.molbrainres.2004.11.014" @default.
- W2060545093 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15790533" @default.
- W2060545093 hasPublicationYear "2005" @default.
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