Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060587989> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W2060587989 endingPage "Fa30" @default.
- W2060587989 startingPage "Fa29" @default.
- W2060587989 abstract "<h3>Background</h3> The fetal lung9s preparation for birth consists of structural and physiologic maturation processes associated with underlying changes in gene expression. Understanding this gene expression program may help to develop novel therapies. Our aim was to identify novel genes up-regulated in the perinatal lung. <h3>Methods</h3> We used the Rae230.2 oligonucleotide array to compare gene expression in embryonic day 16 and 20 rat lungs (n=10, each group). Microarray data were verified by quantitative reverse transcription PCR (qRT-PCR) in the siblings of the animals used for microarray analysis (n=10). The sheep SARG coding sequence was cloned by degenerate oligonucleotide PCR. Gene expression in sheep lungs was analysed by qRT-PCR at 100, 115, 130 and 145 days of gestation (n=5 each). The in vitro steroid induction experiments were performed in the A549 lung epithelial cell line. <h3>Results</h3> Gene expression analysis demonstrated dramatic up-regulation of the gene SARG (‘specifically androgen responsive gene’), a gene of unknown function, in the rat lung during late gestation. Microarray and qRT-PCR analysis showed 32-fold and 42-fold up-regulation, respectively (p<0.0001 for both). The gene was also significantly up-regulated in sheep lungs during late gestation (p<0.01). Maternal dexamethasone treatment increased SARG expression 1.8-fold (p<0.01), fetal adrenalectomy reduced it 1.9-fold (p<0.01). Dexamethasone and dihydro-testosterone both significantly up-regulated SARG in A549 cells in vitro. <h3>Conclusions</h3> SARG is an uncharacterized gene up-regulated in the perinatal lung. SARG expression is increase by both glucocorticoids and androgens. SARG may play a role in physiologic and steroid-mediated lung maturation." @default.
- W2060587989 created "2016-06-24" @default.
- W2060587989 creator A5011851390 @default.
- W2060587989 creator A5014471192 @default.
- W2060587989 creator A5032788156 @default.
- W2060587989 creator A5039169591 @default.
- W2060587989 creator A5048118710 @default.
- W2060587989 creator A5050299817 @default.
- W2060587989 date "2011-06-01" @default.
- W2060587989 modified "2023-09-26" @default.
- W2060587989 title "Dynamic expression and steroid regulation of sarg, a novel gene in the perinatal lung" @default.
- W2060587989 doi "https://doi.org/10.1136/archdischild.2011.300164.44" @default.
- W2060587989 hasPublicationYear "2011" @default.
- W2060587989 type Work @default.
- W2060587989 sameAs 2060587989 @default.
- W2060587989 citedByCount "0" @default.
- W2060587989 crossrefType "journal-article" @default.
- W2060587989 hasAuthorship W2060587989A5011851390 @default.
- W2060587989 hasAuthorship W2060587989A5014471192 @default.
- W2060587989 hasAuthorship W2060587989A5032788156 @default.
- W2060587989 hasAuthorship W2060587989A5039169591 @default.
- W2060587989 hasAuthorship W2060587989A5048118710 @default.
- W2060587989 hasAuthorship W2060587989A5050299817 @default.
- W2060587989 hasConcept C104317684 @default.
- W2060587989 hasConcept C150194340 @default.
- W2060587989 hasConcept C153911025 @default.
- W2060587989 hasConcept C16685009 @default.
- W2060587989 hasConcept C172680121 @default.
- W2060587989 hasConcept C186836561 @default.
- W2060587989 hasConcept C2779234561 @default.
- W2060587989 hasConcept C48023723 @default.
- W2060587989 hasConcept C54355233 @default.
- W2060587989 hasConcept C71924100 @default.
- W2060587989 hasConcept C8415881 @default.
- W2060587989 hasConcept C86803240 @default.
- W2060587989 hasConceptScore W2060587989C104317684 @default.
- W2060587989 hasConceptScore W2060587989C150194340 @default.
- W2060587989 hasConceptScore W2060587989C153911025 @default.
- W2060587989 hasConceptScore W2060587989C16685009 @default.
- W2060587989 hasConceptScore W2060587989C172680121 @default.
- W2060587989 hasConceptScore W2060587989C186836561 @default.
- W2060587989 hasConceptScore W2060587989C2779234561 @default.
- W2060587989 hasConceptScore W2060587989C48023723 @default.
- W2060587989 hasConceptScore W2060587989C54355233 @default.
- W2060587989 hasConceptScore W2060587989C71924100 @default.
- W2060587989 hasConceptScore W2060587989C8415881 @default.
- W2060587989 hasConceptScore W2060587989C86803240 @default.
- W2060587989 hasIssue "Supplement 1" @default.
- W2060587989 hasLocation W20605879891 @default.
- W2060587989 hasOpenAccess W2060587989 @default.
- W2060587989 hasPrimaryLocation W20605879891 @default.
- W2060587989 hasRelatedWork W1983994322 @default.
- W2060587989 hasRelatedWork W2356489734 @default.
- W2060587989 hasRelatedWork W2362238002 @default.
- W2060587989 hasRelatedWork W2364531334 @default.
- W2060587989 hasRelatedWork W2373252446 @default.
- W2060587989 hasRelatedWork W2374855950 @default.
- W2060587989 hasRelatedWork W2415025170 @default.
- W2060587989 hasRelatedWork W2900947575 @default.
- W2060587989 hasRelatedWork W2996351169 @default.
- W2060587989 hasRelatedWork W2998765584 @default.
- W2060587989 hasVolume "96" @default.
- W2060587989 isParatext "false" @default.
- W2060587989 isRetracted "false" @default.
- W2060587989 magId "2060587989" @default.
- W2060587989 workType "article" @default.