Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060659673> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2060659673 endingPage "531a" @default.
- W2060659673 startingPage "531a" @default.
- W2060659673 abstract "Voltage sensing in Kv channels originates from the coupling of movement between the charged S4 segment and the activation gate at the cytoplasmic region of the pore domain. The voltage sensor moves prior to the pore opening and the pore must shut before the voltage sensor returns to its resting state. However, gating current recordings from Kv channels indicate that frequently voltage sensors return more slowly after depolarisations that populate open states, indicating that the open pore exerts a resistance to S4 return. This process of pore closure therefore intrinsically regulates the deactivation kinetics of Kv channels. We observed slow voltage sensor return (IgOFF) in WT-Kv1.2 channels under non-permeant ionic conditions after depolarisations to voltages that caused channel openings. Using TEA+ and NMG+ internal solutions resulted in a slower IgOFF than internal Cs+, suggesting that the intracellular ionic composition was modulating IgOFF. A mutation in the pore lining S6 segment to enlarge the inner cavity (Kv1.2-I402C) removed the slowing of IgOFF in the presence of internal NMG+, suggesting that NMG+ interacted within the inner cavity of the WT channel to prevent pore closure through a ‘foot in the door’ mechanism. Gating currents of a non-conducting, P-type inactivated channel (Kv1.2-W366F, V381T), in the presence of intracellular K+ ions also displayed a slowing of IgOFF after depolarisations that would open the channel pore. These results suggest that internal K+ ions bound in the inner cavity can also slow activation gate closure. We propose that internal ions in the cavity of Kv1.2 allosterically regulate the voltage sensor deactivation kinetics by preventing pore closure and thus rate limiting the return of voltage sensors." @default.
- W2060659673 created "2016-06-24" @default.
- W2060659673 creator A5019990104 @default.
- W2060659673 creator A5029781869 @default.
- W2060659673 creator A5030291558 @default.
- W2060659673 creator A5083962653 @default.
- W2060659673 date "2012-01-01" @default.
- W2060659673 modified "2023-09-29" @default.
- W2060659673 title "Intracellular Ions Impede Voltage Sensor Return in Kv1.2 Channels" @default.
- W2060659673 doi "https://doi.org/10.1016/j.bpj.2011.11.2901" @default.
- W2060659673 hasPublicationYear "2012" @default.
- W2060659673 type Work @default.
- W2060659673 sameAs 2060659673 @default.
- W2060659673 citedByCount "0" @default.
- W2060659673 crossrefType "journal-article" @default.
- W2060659673 hasAuthorship W2060659673A5019990104 @default.
- W2060659673 hasAuthorship W2060659673A5029781869 @default.
- W2060659673 hasAuthorship W2060659673A5030291558 @default.
- W2060659673 hasAuthorship W2060659673A5083962653 @default.
- W2060659673 hasBestOaLocation W20606596731 @default.
- W2060659673 hasConcept C121332964 @default.
- W2060659673 hasConcept C12554922 @default.
- W2060659673 hasConcept C131584629 @default.
- W2060659673 hasConcept C145148216 @default.
- W2060659673 hasConcept C165801399 @default.
- W2060659673 hasConcept C170493617 @default.
- W2060659673 hasConcept C178790620 @default.
- W2060659673 hasConcept C185592680 @default.
- W2060659673 hasConcept C191897082 @default.
- W2060659673 hasConcept C192562407 @default.
- W2060659673 hasConcept C194544171 @default.
- W2060659673 hasConcept C50254741 @default.
- W2060659673 hasConcept C55493867 @default.
- W2060659673 hasConcept C62520636 @default.
- W2060659673 hasConcept C79879829 @default.
- W2060659673 hasConcept C86803240 @default.
- W2060659673 hasConceptScore W2060659673C121332964 @default.
- W2060659673 hasConceptScore W2060659673C12554922 @default.
- W2060659673 hasConceptScore W2060659673C131584629 @default.
- W2060659673 hasConceptScore W2060659673C145148216 @default.
- W2060659673 hasConceptScore W2060659673C165801399 @default.
- W2060659673 hasConceptScore W2060659673C170493617 @default.
- W2060659673 hasConceptScore W2060659673C178790620 @default.
- W2060659673 hasConceptScore W2060659673C185592680 @default.
- W2060659673 hasConceptScore W2060659673C191897082 @default.
- W2060659673 hasConceptScore W2060659673C192562407 @default.
- W2060659673 hasConceptScore W2060659673C194544171 @default.
- W2060659673 hasConceptScore W2060659673C50254741 @default.
- W2060659673 hasConceptScore W2060659673C55493867 @default.
- W2060659673 hasConceptScore W2060659673C62520636 @default.
- W2060659673 hasConceptScore W2060659673C79879829 @default.
- W2060659673 hasConceptScore W2060659673C86803240 @default.
- W2060659673 hasIssue "3" @default.
- W2060659673 hasLocation W20606596731 @default.
- W2060659673 hasOpenAccess W2060659673 @default.
- W2060659673 hasPrimaryLocation W20606596731 @default.
- W2060659673 hasRelatedWork W1964623499 @default.
- W2060659673 hasRelatedWork W1967113088 @default.
- W2060659673 hasRelatedWork W1988759455 @default.
- W2060659673 hasRelatedWork W2030310238 @default.
- W2060659673 hasRelatedWork W2122857255 @default.
- W2060659673 hasRelatedWork W2154875971 @default.
- W2060659673 hasRelatedWork W2585182539 @default.
- W2060659673 hasRelatedWork W3130077175 @default.
- W2060659673 hasRelatedWork W3132007339 @default.
- W2060659673 hasRelatedWork W3183158762 @default.
- W2060659673 hasVolume "102" @default.
- W2060659673 isParatext "false" @default.
- W2060659673 isRetracted "false" @default.
- W2060659673 magId "2060659673" @default.
- W2060659673 workType "article" @default.