Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060674920> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W2060674920 endingPage "1125" @default.
- W2060674920 startingPage "1120" @default.
- W2060674920 abstract "국내에 자생 중인 큰엉겅퀴(Cirsium pendulum)를 강원도의 홍천, 원주, 평창, 양양 및 경기도 가평과 충청북도의 충주에서 채취하고, 고려엉겅퀴(Cirsium setidens)를 강원도의 태백 및 경상북도의 봉화에서 채취하였다. 채취된 식물들의 genomic DNA를 분리하여서 18S rDNA, ITS1, 5.8S rDNA, ITS2 및 28S rDNA의 일부를 증폭하고, 그 염기서열을 분석하였다. 이렇게 얻어진 염기서열을 GenBank에 등록하고, 서로 비교해 보았다. 그 결과 큰엉겅퀴는 6개체 모두에서 18S rDNA 및 5.8S rDNA의 염기서열은 서로 완전히 일치하고 있으나, ITS1과 ITS2의 염기서열에서는 약간의 차이를 보였다. 고려엉겅퀴의 경우에는 18S rDNA, ITS1 및 ITS2에서 2개체가 서로 비교적 큰 차이를 보였다. 일본의 홋카이도에서 채취된 큰엉겅퀴와 중국의 안휘성에서 채취된 엉겅퀴(Cirsium japonicum)를 포함하여서 ITS1과 ITS2 염기서열을 비교한 결과, 엉겅퀴, 큰엉겅퀴, 고려엉겅퀴는 확연하게 서로 다른 그룹을 형성하는 것으로 나타났다. 또한 채취된 큰엉겅퀴 및 고려엉겅퀴들의 silymarin 함량을 분석해 본 결과, 모두에서 그 함량이 2 mg/ml가 넘는 것으로 나타났다. 따라서 엉겅퀴뿐만 아니라 큰엉겅퀴나 고려엉겅퀴도 여러 가지 약리작용을 가진 것으로 보고된 silymarin을 상당히 높은 농도로 함유하고 있는 것으로 나타났다. Cirsium pendulum plants were collected from Hongcheon, Pyeongchang, Wonju, Yangyang in Kangwondo, Gapyeong in Gyeongkido, and Choongju in Choongcheongbukdo. Cirsium setidens plants were collected from Taebaek in Kangwondo and Bonghwa in Kyeongsangbukdo. Genomic DNA was prepared from those plants and used for the amplification of 18S rDNA, ITS1, 5.8S rDNA, ITS2, and part of 28S rDNA. The PCR products were sequenced, and the sequence was deposited in the GenBank. The comparison of those sequences has revealed that the rDNA sequences are identical for all six C. pendulum plants, but that the ITS1 and ITS2 sequences contain variable nucleotides. The two C. setidens plants had different nucleotides in 18S rDNA, ITS1, and ITS2. The comparison of the DNA sequences of C. pendulum and C. setidens collected in this study with C. pendulum of Hokkaido in Japan and C. japonicum of Anhui in China indicated that the plants of those three species are clearly divided into three distinct groups. The silymarin content of the collected plants was analyzed and turned out to be quite high. Therefore, it has been found that both C. pendulum and C. setidens plants are producing large amounts of silymarin, which has been reported to have various medicinal effects." @default.
- W2060674920 created "2016-06-24" @default.
- W2060674920 creator A5004977115 @default.
- W2060674920 creator A5075146226 @default.
- W2060674920 date "2012-08-30" @default.
- W2060674920 modified "2023-10-03" @default.
- W2060674920 title "Phylogenetic and Chemical Analyses of Cirsium pendulum and Cirsium setidens Inhabiting Korea" @default.
- W2060674920 cites W2016634372 @default.
- W2060674920 cites W2025910569 @default.
- W2060674920 cites W2046171565 @default.
- W2060674920 cites W2095724872 @default.
- W2060674920 cites W2107686567 @default.
- W2060674920 cites W2132632499 @default.
- W2060674920 cites W2148904300 @default.
- W2060674920 cites W2151471884 @default.
- W2060674920 cites W2157335302 @default.
- W2060674920 cites W892718978 @default.
- W2060674920 doi "https://doi.org/10.5352/jls.2012.22.8.1120" @default.
- W2060674920 hasPublicationYear "2012" @default.
- W2060674920 type Work @default.
- W2060674920 sameAs 2060674920 @default.
- W2060674920 citedByCount "10" @default.
- W2060674920 countsByYear W20606749202012 @default.
- W2060674920 countsByYear W20606749202014 @default.
- W2060674920 countsByYear W20606749202015 @default.
- W2060674920 countsByYear W20606749202017 @default.
- W2060674920 countsByYear W20606749202018 @default.
- W2060674920 countsByYear W20606749202020 @default.
- W2060674920 countsByYear W20606749202022 @default.
- W2060674920 crossrefType "journal-article" @default.
- W2060674920 hasAuthorship W2060674920A5004977115 @default.
- W2060674920 hasAuthorship W2060674920A5075146226 @default.
- W2060674920 hasBestOaLocation W20606749201 @default.
- W2060674920 hasConcept C104317684 @default.
- W2060674920 hasConcept C193252679 @default.
- W2060674920 hasConcept C207812311 @default.
- W2060674920 hasConcept C54355233 @default.
- W2060674920 hasConcept C59822182 @default.
- W2060674920 hasConcept C79029880 @default.
- W2060674920 hasConcept C86803240 @default.
- W2060674920 hasConceptScore W2060674920C104317684 @default.
- W2060674920 hasConceptScore W2060674920C193252679 @default.
- W2060674920 hasConceptScore W2060674920C207812311 @default.
- W2060674920 hasConceptScore W2060674920C54355233 @default.
- W2060674920 hasConceptScore W2060674920C59822182 @default.
- W2060674920 hasConceptScore W2060674920C79029880 @default.
- W2060674920 hasConceptScore W2060674920C86803240 @default.
- W2060674920 hasIssue "8" @default.
- W2060674920 hasLocation W20606749201 @default.
- W2060674920 hasOpenAccess W2060674920 @default.
- W2060674920 hasPrimaryLocation W20606749201 @default.
- W2060674920 hasRelatedWork W2022672552 @default.
- W2060674920 hasRelatedWork W2026385282 @default.
- W2060674920 hasRelatedWork W2051662717 @default.
- W2060674920 hasRelatedWork W2082510711 @default.
- W2060674920 hasRelatedWork W2322131235 @default.
- W2060674920 hasRelatedWork W2373731554 @default.
- W2060674920 hasRelatedWork W2387199019 @default.
- W2060674920 hasRelatedWork W2402770443 @default.
- W2060674920 hasRelatedWork W3161267193 @default.
- W2060674920 hasRelatedWork W4234628617 @default.
- W2060674920 hasVolume "22" @default.
- W2060674920 isParatext "false" @default.
- W2060674920 isRetracted "false" @default.
- W2060674920 magId "2060674920" @default.
- W2060674920 workType "article" @default.