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- W2060675878 abstract "Preparative counter-current chromatography (CCC) purification of valrubicin (AD-32) from crude synthetic drug has been successfully performed for the first time using upright coil planet centrifuge with four multilayer coils connected in series with 1600 ml capacity. The two-phase system used was composed of light petroleum (bp 60-90 degrees C)-ethyl acetate-tetrachloromethane-methanol-water at an optimized volume ratio of 1:1:8:6:1. Target compound (1.2 g) with a purity of 99.88% was obtained from 1.5 g of crude synthetic drug with a purity 95.49% based on HPLC peak area percentage. Identification of the target compound was performed by electrospray ionization mass spectrometry, one- and two-dimensional nuclear magnetic resonance." @default.
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- W2060675878 date "2005-05-01" @default.
- W2060675878 modified "2023-10-16" @default.
- W2060675878 title "Preparative counter-current chromatography purification of valrubicin (AD-32) from crude synthetic drug using upright coil planet centrifuge with four multilayer coils connected in series" @default.
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- W2060675878 doi "https://doi.org/10.1016/j.chroma.2005.04.027" @default.
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