Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060720286> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2060720286 endingPage "16015" @default.
- W2060720286 startingPage "16008" @default.
- W2060720286 abstract "The specific catalytic roles of two groups of DNA polymerase beta active site residues identified from crystal structures were investigated: residues possibly involved in DNA template positioning (Lys280, Asn294, and Glu295) and residues possibly involved in binding the triphosphate moiety of the incoming dNTP (Arg149, Ser180, Arg183, and Ser188). Eight site-specific mutants were constructed: K280A, N294A, N294Q, E295A, R149A, S180A, R183A, and S188A. Two-dimensional NMR analysis was employed to show that the global conformation of the mutants has not been perturbed significantly. Pre-steady-state kinetic analyses with single-nucleotide gapped DNA substrates were then performed to obtain the rate of catalysis at saturating dNTP (k(pol)), the apparent dissociation constant for dNTP (K(d)), catalytic efficiency k(pol)/K(d), and fidelity. Of the three template-positioning residues, Asn294 and Glu295 (but not Lys280) contribute significantly to k(pol). Taken together with other data, the results suggest that these two residues help to stabilize the transition state during catalysis even though they interact with the DNA template backbone rather than directly with the incoming dNTP or the opposite base on the template. Furthermore, the fidelity increases by up to 19-fold for N294Q due to differential k(pol) effects between correct and incorrect nucleotides. Of the four potential triphosphate-binding residues, Ser180 and Arg183 contribute significantly to k(pol) while the effects of R149A are relatively small and are primarily on K(d), and Ser188 appears to play a minimal role in the catalysis by Pol beta. These results identify several residues important for catalysis and quantitate the contributions of each of those residues. The functional data are discussed in relation to the prediction on the basis of available crystal structures." @default.
- W2060720286 created "2016-06-24" @default.
- W2060720286 creator A5046326187 @default.
- W2060720286 creator A5057117256 @default.
- W2060720286 creator A5077669538 @default.
- W2060720286 creator A5083030027 @default.
- W2060720286 creator A5086432639 @default.
- W2060720286 date "2000-11-23" @default.
- W2060720286 modified "2023-10-09" @default.
- W2060720286 title "DNA Polymerase β: Contributions of Template-Positioning and dNTP Triphosphate-Binding Residues to Catalysis and Fidelity" @default.
- W2060720286 cites W2065140867 @default.
- W2060720286 doi "https://doi.org/10.1021/bi0008480" @default.
- W2060720286 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11123928" @default.
- W2060720286 hasPublicationYear "2000" @default.
- W2060720286 type Work @default.
- W2060720286 sameAs 2060720286 @default.
- W2060720286 citedByCount "47" @default.
- W2060720286 countsByYear W20607202862012 @default.
- W2060720286 countsByYear W20607202862013 @default.
- W2060720286 countsByYear W20607202862014 @default.
- W2060720286 countsByYear W20607202862015 @default.
- W2060720286 countsByYear W20607202862016 @default.
- W2060720286 countsByYear W20607202862017 @default.
- W2060720286 countsByYear W20607202862018 @default.
- W2060720286 countsByYear W20607202862020 @default.
- W2060720286 countsByYear W20607202862022 @default.
- W2060720286 crossrefType "journal-article" @default.
- W2060720286 hasAuthorship W2060720286A5046326187 @default.
- W2060720286 hasAuthorship W2060720286A5057117256 @default.
- W2060720286 hasAuthorship W2060720286A5077669538 @default.
- W2060720286 hasAuthorship W2060720286A5083030027 @default.
- W2060720286 hasAuthorship W2060720286A5086432639 @default.
- W2060720286 hasConcept C104317684 @default.
- W2060720286 hasConcept C107824862 @default.
- W2060720286 hasConcept C12554922 @default.
- W2060720286 hasConcept C131140672 @default.
- W2060720286 hasConcept C143065580 @default.
- W2060720286 hasConcept C185592680 @default.
- W2060720286 hasConcept C2756471 @default.
- W2060720286 hasConcept C512185932 @default.
- W2060720286 hasConcept C552990157 @default.
- W2060720286 hasConcept C55493867 @default.
- W2060720286 hasConcept C71240020 @default.
- W2060720286 hasConcept C8010536 @default.
- W2060720286 hasConcept C82381507 @default.
- W2060720286 hasConcept C86803240 @default.
- W2060720286 hasConceptScore W2060720286C104317684 @default.
- W2060720286 hasConceptScore W2060720286C107824862 @default.
- W2060720286 hasConceptScore W2060720286C12554922 @default.
- W2060720286 hasConceptScore W2060720286C131140672 @default.
- W2060720286 hasConceptScore W2060720286C143065580 @default.
- W2060720286 hasConceptScore W2060720286C185592680 @default.
- W2060720286 hasConceptScore W2060720286C2756471 @default.
- W2060720286 hasConceptScore W2060720286C512185932 @default.
- W2060720286 hasConceptScore W2060720286C552990157 @default.
- W2060720286 hasConceptScore W2060720286C55493867 @default.
- W2060720286 hasConceptScore W2060720286C71240020 @default.
- W2060720286 hasConceptScore W2060720286C8010536 @default.
- W2060720286 hasConceptScore W2060720286C82381507 @default.
- W2060720286 hasConceptScore W2060720286C86803240 @default.
- W2060720286 hasIssue "51" @default.
- W2060720286 hasLocation W20607202861 @default.
- W2060720286 hasLocation W20607202862 @default.
- W2060720286 hasOpenAccess W2060720286 @default.
- W2060720286 hasPrimaryLocation W20607202861 @default.
- W2060720286 hasRelatedWork W1996851263 @default.
- W2060720286 hasRelatedWork W2015471659 @default.
- W2060720286 hasRelatedWork W2064291905 @default.
- W2060720286 hasRelatedWork W2092998957 @default.
- W2060720286 hasRelatedWork W2114345540 @default.
- W2060720286 hasRelatedWork W2126915623 @default.
- W2060720286 hasRelatedWork W2144168750 @default.
- W2060720286 hasRelatedWork W2194888552 @default.
- W2060720286 hasRelatedWork W4234093616 @default.
- W2060720286 hasRelatedWork W909894712 @default.
- W2060720286 hasVolume "39" @default.
- W2060720286 isParatext "false" @default.
- W2060720286 isRetracted "false" @default.
- W2060720286 magId "2060720286" @default.
- W2060720286 workType "article" @default.