Matches in SemOpenAlex for { <https://semopenalex.org/work/W2060751446> ?p ?o ?g. }
- W2060751446 endingPage "674" @default.
- W2060751446 startingPage "665" @default.
- W2060751446 abstract "Based on BLAST analysis of the human and mouse genome databases using the human CMP sialic acid; α2,8-sialyltransferase cDNA (hST8Sia I; EC 2.4.99.8), a putative sialyltransferase gene, was identified on human chromosome 10. The genomic organization was found to be similar to that of hST8Sia I and hST8Sia V. Transcriptional expression analysis showed that the newly identified gene was constitutively expressed at low levels in various human tissues and cell lines. We have isolated a full-length cDNA clone from the breast cancer cell line MCF-7 that encoded a type II membrane protein of 398 amino acid residues with the conserved motifs of sialyltransferases. We have established a mammary cell line (MDA-MB-231) stably transfected with the full-length hST8Sia VI and the analysis of sialylated carbohydrate structures expressed at the cell surface clearly indicated the disappearance of Neu5Acα2-3-sialylated structures. The transient expression of a truncated soluble form of the enzyme in either COS-7 cells or insect Sf-9 cells led to the production of an active enzyme in which substrate specificity was determined. Detailed substrate specificity analysis of the hST8Sia VI recombinant enzyme in vitro, revealed that this enzyme required the trisaccharide Neu5Acα2-3Galβ1-3GalNAc (where Neu5Ac is N-acetylneuraminic acid and GalNAc is N-acetylgalactosamine) to generate diSia (disialic acid) motifs specifically on O-glycans." @default.
- W2060751446 created "2016-06-24" @default.
- W2060751446 creator A5011654247 @default.
- W2060751446 creator A5018408053 @default.
- W2060751446 creator A5023819887 @default.
- W2060751446 creator A5051000352 @default.
- W2060751446 creator A5054254122 @default.
- W2060751446 creator A5068872279 @default.
- W2060751446 creator A5084208325 @default.
- W2060751446 date "2005-12-06" @default.
- W2060751446 modified "2023-09-24" @default.
- W2060751446 title "Molecular cloning and expression of a human hST8Sia VI (α2,8-sialyltransferase) responsible for the synthesis of the diSia motif on O-glycosylproteins" @default.
- W2060751446 cites W1487427036 @default.
- W2060751446 cites W1507147440 @default.
- W2060751446 cites W1521257112 @default.
- W2060751446 cites W1599773752 @default.
- W2060751446 cites W1604424618 @default.
- W2060751446 cites W1604742230 @default.
- W2060751446 cites W1655461206 @default.
- W2060751446 cites W1729319996 @default.
- W2060751446 cites W1877595444 @default.
- W2060751446 cites W1976152450 @default.
- W2060751446 cites W1983833224 @default.
- W2060751446 cites W1985631817 @default.
- W2060751446 cites W1986044155 @default.
- W2060751446 cites W1986048273 @default.
- W2060751446 cites W1987253015 @default.
- W2060751446 cites W1992304266 @default.
- W2060751446 cites W2000518846 @default.
- W2060751446 cites W2007760520 @default.
- W2060751446 cites W2009482334 @default.
- W2060751446 cites W2014602126 @default.
- W2060751446 cites W2017726307 @default.
- W2060751446 cites W2018366006 @default.
- W2060751446 cites W2019062483 @default.
- W2060751446 cites W2023524874 @default.
- W2060751446 cites W2033269692 @default.
- W2060751446 cites W2041196497 @default.
- W2060751446 cites W2046031000 @default.
- W2060751446 cites W2046203969 @default.
- W2060751446 cites W2048553094 @default.
- W2060751446 cites W2050120618 @default.
- W2060751446 cites W2056139556 @default.
- W2060751446 cites W2057241909 @default.
- W2060751446 cites W2057438794 @default.
- W2060751446 cites W2057805791 @default.
- W2060751446 cites W2064859977 @default.
- W2060751446 cites W2068746509 @default.
- W2060751446 cites W2092754814 @default.
- W2060751446 cites W2095879241 @default.
- W2060751446 cites W2107163098 @default.
- W2060751446 cites W2116261764 @default.
- W2060751446 cites W2117426164 @default.
- W2060751446 cites W2119583049 @default.
- W2060751446 cites W2120982884 @default.
- W2060751446 cites W2153950787 @default.
- W2060751446 cites W2158714788 @default.
- W2060751446 cites W230847824 @default.
- W2060751446 cites W2415249527 @default.
- W2060751446 cites W248409975 @default.
- W2060751446 doi "https://doi.org/10.1042/bj20051120" @default.
- W2060751446 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1770850" @default.
- W2060751446 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16120058" @default.
- W2060751446 hasPublicationYear "2005" @default.
- W2060751446 type Work @default.
- W2060751446 sameAs 2060751446 @default.
- W2060751446 citedByCount "35" @default.
- W2060751446 countsByYear W20607514462012 @default.
- W2060751446 countsByYear W20607514462013 @default.
- W2060751446 countsByYear W20607514462014 @default.
- W2060751446 countsByYear W20607514462015 @default.
- W2060751446 countsByYear W20607514462016 @default.
- W2060751446 countsByYear W20607514462018 @default.
- W2060751446 countsByYear W20607514462019 @default.
- W2060751446 countsByYear W20607514462020 @default.
- W2060751446 countsByYear W20607514462021 @default.
- W2060751446 countsByYear W20607514462022 @default.
- W2060751446 crossrefType "journal-article" @default.
- W2060751446 hasAuthorship W2060751446A5011654247 @default.
- W2060751446 hasAuthorship W2060751446A5018408053 @default.
- W2060751446 hasAuthorship W2060751446A5023819887 @default.
- W2060751446 hasAuthorship W2060751446A5051000352 @default.
- W2060751446 hasAuthorship W2060751446A5054254122 @default.
- W2060751446 hasAuthorship W2060751446A5068872279 @default.
- W2060751446 hasAuthorship W2060751446A5084208325 @default.
- W2060751446 hasBestOaLocation W20607514464 @default.
- W2060751446 hasConcept C104317684 @default.
- W2060751446 hasConcept C153911025 @default.
- W2060751446 hasConcept C181199279 @default.
- W2060751446 hasConcept C185592680 @default.
- W2060751446 hasConcept C187882448 @default.
- W2060751446 hasConcept C189482833 @default.
- W2060751446 hasConcept C19924922 @default.
- W2060751446 hasConcept C2777187973 @default.
- W2060751446 hasConcept C2778322149 @default.
- W2060751446 hasConcept C2780115458 @default.
- W2060751446 hasConcept C54355233 @default.
- W2060751446 hasConcept C55493867 @default.