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• W2060751966 endingPage "305" @default.
• W2060751966 startingPage "300" @default.
• W2060751966 abstract "Abstract In this study, the growth factors in COLLOSS®E were analyzed, using ELISA tests, mass spectrometry, western blotting, and a 24‐day cell culture experiment using osteoblast‐like cells. The results of the ELISA testing, mass spectrometry, and western blotting all confirmed that TGF‐β1 was the main growth factor in COLLOSS®E at 55 ng/mg. The results from the culture test showed that the cell proliferation, alkaline phosphatase activity, and matrix calcification were all drastically changed by the addition of COLLOSS®E, mirroring the effects of addition of TGF‐β1. We conclude that COLLOSS®E is not only a rich source of TGFβ‐1, but also contains the growth factors TGFβ‐2, BMP‐2, BMP‐3, BMP‐7, IGF‐1, and possibly VEGF. Other growth factors might be present in COLLOSS®E, but were not identified due to inherent detection limits of the used ELISA and mass spectrometry techniques. The number of osteoinductive factors in COLLOSS®E causes a synergistic effect, explaining the new bone formation found in previously described in vivo studies, with much lower growth factor concentrations when compared with recombinant BMPs. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009" @default.
• W2060751966 created "2016-06-24" @default.
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• W2060751966 date "2008-09-29" @default.
• W2060751966 modified "2023-10-07" @default.
• W2060751966 title "Analytical assessment of the osteoinductive material COLLOSS®E" @default.
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• W2060751966 doi "https://doi.org/10.1002/jbm.b.31216" @default.
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