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• W2060758897 abstract "Abstract— Relative potency of 10 fluorescein derivatives as sensitizers of delayed photohemolysis of human erythrocytes has been assessed. Dilute suspensions of washed cells were illuminated in the presence of sensitizer at different concentrations for 1 h and analyzed for percent hemolysis following 23 h of dark incubation. Plots of percent hemolysis versus concentration showed a steep dependence on concentration for all sensitizers. Additional measurements of octanol/water partition coefficients, photon absorption in octanol and in saline and photobleach rates were made. After correction for absorption cross section, the effectiveness values ranged over more than three orders of magnitude with fluorescein being the least potent and rose bengal the most. A reasonable prediction of potency is obtained by taking the product of partition coefficient, relative absorption in octanol versus water and molecular weight of the substituents added to the fluorescein skeleton. The results suggest that the influence of halogen substitution on sensitizing potency is exerted by four factors; (1) distribution of sensitizer into a low polarity region of the cell membrane, (2) absorption efficiency in a low polarity region, (3) triplet quantum yield, and (4) photobleach rate." @default.
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• W2060758897 date "2008-01-02" @default.
• W2060758897 modified "2023-09-27" @default.
• W2060758897 title "CELL MEMBRANE PHOTOMODIFICATION: RELATIVE EFFECTIVENESS OF HALOGENATED FLUORESCEINS FOR PHOTOHEMOLYSIS" @default.
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• W2060758897 doi "https://doi.org/10.1111/j.1751-1097.1982.tb02572.x" @default.
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