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- W2060834819 abstract "In this study, an adaptive laboratory evolution strategy was originally developed to enhance fermentative hydrogen production by directionally regulating the metabolic heterogeneity in anaerobic mixed culture. The results indicated that the co-introduction of 4-methylpyrazole and oxamate could redistribute the metabolic flux to butyrate-type hydrogen fermentation. Subsequently, a synergistic evolutionary pressure, combining exogenous butyrate stress with 4-methylpyrazole and oxamate, was employed to evolve hydrogen-producing mixed culture with continuous fermentation system. The metabolic engineering strategy could directionally regulate the metabolic heterogeneity through efficiently shaping powerful butyrate-type hydrogen-producing community, by which evolved culture acquired a significantly improved hydrogen yield and productivity. Furthermore, compared with original culture, evolved culture possessed much higher activities of pyruvate-ferredoxin oxidoreductase and hydrogenase but a much lower ferredoxin-NAD+ oxidoreductase activity, and these enzymatic evolutionary mechanisms were crucially important for the enhanced hydrogen fermentation." @default.
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- W2060834819 date "2010-11-01" @default.
- W2060834819 modified "2023-09-27" @default.
- W2060834819 title "Metabolic modeling of polyhydroxyalkanoates (PHA) production from complex mixtures of competing volatile fatty acids (VFA) by mixed microbial cultures (MMC)" @default.
- W2060834819 doi "https://doi.org/10.1016/j.jbiotec.2010.10.035" @default.
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