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• W2060864671 endingPage "32" @default.
• W2060864671 startingPage "25" @default.
• W2060864671 abstract "Recoverin is cotranslationally modified by the covalent linkage of a myristoyl group to its N terminus. It is a member of a family of Ca2+-myristoyl switch proteins. Recombinant myristoylated revoverin is currently produced by the cotransformation of bacteria with recoverin and an enzyme that allows N-myristoylation and by supplementing the culture medium with myristic acid. A large variation in the myristoylation level of recoverin and in the amount of myristic acid supplied to the culture medium can be found in the literature. Moreover, although it is known to strongly affect bacterial growth, the amount of ethanol used to solubilize myristic acid is only scarcely mentioned. To improve our understanding of the parameters responsible for recombinant recoverin myristoylation, the effects of myristic acid and ethanol on recoverin myristoylation and expression levels have been systematically studied. In addition, a single-step purification procedure to produce purified myristoylated and nonmyristoylated recombinant recoverin has also been devised. Finally, sodium myristate has been used as an efficient alternative substrate to achieve high myristoylation and expression levels of recoverin. Given that a large number of proteins are myristoylated, these procedures could be applied to several other proteins in addition to recoverin." @default.
• W2060864671 created "2016-06-24" @default.
• W2060864671 creator A5010869173 @default.
• W2060864671 creator A5032228278 @default.
• W2060864671 creator A5078639626 @default.
• W2060864671 date "2006-02-01" @default.
• W2060864671 modified "2023-10-03" @default.
• W2060864671 title "Single-step purification of myristoylated and nonmyristoylated recoverin and substrate dependence of myristoylation level" @default.
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• W2060864671 doi "https://doi.org/10.1016/j.ab.2005.11.023" @default.
• W2060864671 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16376289" @default.
• W2060864671 hasPublicationYear "2006" @default.
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