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• W2060996945 abstract "Approximately 60% of emerging viruses are of zoonotic origin, with three-fourths derived from wild animals. Many of these zoonotic diseases are transmitted by rodents with important information about their reservoir dynamics and pathogenesis missing. One main reason for the gap in our knowledge is the lack of adequate cell culture systems as models for the investigation of rodent-borne (robo) viruses in vitro. Therefore we established and characterized a new cell line, BVK168, using the kidney of a bank vole, Myodes glareolus, the most abundant member of the Arvicolinae trapped in Germany. BVK168 proved to be of epithelial morphology expressing tight junctions as well as adherence junction proteins. The BVK168 cells were analyzed for their infectability by several arbo- and robo-viruses: Vesicular stomatitis virus, vaccinia virus, cowpox virus, Sindbis virus, Pixuna virus, Usutu virus, Inkoo virus, Puumalavirus, and Borna disease virus (BDV). The cell line was susceptible for all tested viruses, and most interestingly also for the difficult to propagate BDV. In conclusion, the newly established cell line from wildlife rodents seems to be an excellent tool for the isolation and characterization of new rodent-associated viruses and may be used as in vitro- model to study properties and pathogenesis of these agents." @default.
• W2060996945 created "2016-06-24" @default.
• W2060996945 creator A5016343037 @default.
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• W2060996945 date "2011-07-05" @default.
• W2060996945 modified "2023-10-10" @default.
• W2060996945 title "A new permanent cell line derived from the bank vole (Myodes glareolus) as cell culture model for zoonotic viruses" @default.
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• W2060996945 doi "https://doi.org/10.1186/1743-422x-8-339" @default.
• W2060996945 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3145595" @default.
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