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- W2061026108 abstract "The maltose phosphorylase (MPase) gene of Bacillus sp. strain RK-1 was cloned by PCR with oligonucleotide primers designed on the basis of a partial N-terminal amino acid sequence of the purified enzyme. The MPase gene consisted of 2,655 bp encoding a theoretical protein with a Mr of 88,460, and had no secretion signal sequence, although most of the MPase activity was detected in the culture supernatant of RK-1. This cloned MPase gene and the trehalose phosphorylase (TPase) gene from Bacillus stearothermophilus SK-1 were efficiently expressed intracellularly under the control of the Bacillus amyloliquefaciens alpha-amylase promoter in Bacillus subtilis. The production yields were estimated to be more than 2 g of enzyme per liter of medium, about 250 times the production of the original strains, in a simple shake flask. About 60% of maltose was converted into trehalose by the simultaneous action of both enzymes produced in B. subtilis." @default.
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- W2061026108 date "2002-01-01" @default.
- W2061026108 modified "2023-10-17" @default.
- W2061026108 title "Cloning of the Maltose Phosphorylase Gene from<i>Bacillus</i>sp. Strain RK-1 and Efficient Production of the Cloned Gene and the Trehalose Phosphorylase Gene from…" @default.
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- W2061026108 doi "https://doi.org/10.1271/bbb.66.2594" @default.
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