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- W2061045772 abstract "Chlorella sorokiniana may prove to be a useful organism in which to express genes/cDNAs from certain higher plants encoding enzymes catalyzing the synthesis of compounds of medical or industrial importance. This organism has a NADP-specific glutamate dehydrogenase (NADP-GDH) nuclear-gene encoding a precursor mRNA that appears to be alternatively spliced to yield mRNAs differing in size and encoding enzyme subunits with differing affinities for ammonium. The promoter of this gene may be useful to drive the expression of heterologous genes. The transcription initiation site of the NADP-GDH gene was identified. This site delineates the 3′-border of the promoter region, and 1285 bp upstream from this site have been sequenced. To identify the promoter 5′-border by deletion analysis, this region (coupled to a reporter gene in a plasmid vector) was to be introduced by electroporation intoC. sorokiniana protoplasts. Cells were treated with a mixture of hydrolytic enzymes; however, non-hydrolyzable component(s) in the cell wall inhibited protoplast formation. The cell wall was subsequently shown to contain acetolysis-resistant component(s) identified as sporopollenin-like in other algae. Since sporopollenin was reported earlier to be a polymer of carotenoids/carotenoid-esters, the cells were cultured in the presence of norflurazon, an inhibitor of carotenoid biosynthesis. Whereas synthesis of total carotenoids was inhibited by 75%, synthesis of the acetolysis-resistant component(s) in the cell wall was not inhibited. In addition to the above studies, a revised model for the regulation of expression of theC. sorokiniana NADP-GDH gene is presented." @default.
- W2061045772 created "2016-06-24" @default.
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- W2061045772 date "1994-08-01" @default.
- W2061045772 modified "2023-09-25" @default.
- W2061045772 title "Transcription initiation site of a NADP-specific glutamate dehydrogenase gene and potential use of its promoter region to express foreign genes in ammonium-culturedChlorella sorokiniana cells" @default.
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- W2061045772 doi "https://doi.org/10.1007/bf02182164" @default.
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