FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061057944> ?p ?o ?g. }

• W2061057944 endingPage "800" @default.
• W2061057944 startingPage "793" @default.
• W2061057944 abstract "Enolase is a dimeric metal-activated metalloenzyme which uses two magnesium ions per subunit: the strongly bound conformational ion and the catalytic ion that binds to the enzyme−substrate complex inducing catalysis. The crystal structure of the human neuronal enolase−Mg2F2Pi complex (enolase fluoride/phosphate inhibitory complex, EFPIC) determined at 1.36 Å resolution shows that the combination of anions effectively mimics an intermediate state in catalysis. The phosphate ion binds in the same site as the phosphate group of the substrate/product, 2-phospho-d-glycerate/phosphoenolpyruvate, and induces binding of the catalytic Mg2+ ion. One fluoride ion bridges the structural and catalytic magnesium ions while the other interacts with the structural magnesium ion and the ammonio groups of Lys 342 and Lys 393. These fluoride ion positions correspond closely to the positions of the oxygen atoms of the substrate's carboxylate moiety. To relate structural changes resulting from fluoride, phosphate, and magnesium ions binding to those that are induced by phosphate and magnesium ions alone, we also determined the structure of the human neuronal enolase−Mg2Pi complex (enolase phosphate inhibitory complex, EPIC) at 1.92 Å resolution. It shows the closed conformation in one subunit and a mixture of open and semiclosed conformations in the other. The EPFIC dimer is essentially symmetric while the EPIC dimer is asymmetric. Isothermal titration calorimetry data confirmed binding of four fluoride ions per dimer and yielded Kb values of 7.5 × 105 ± 1.3 × 105, 1.2 × 105 ± 0.2 × 105, 8.6 × 104 ± 1.6 × 104, and 1.6 × 104 ± 0.7 × 104 M-1. The different binding constants indicate negative cooperativity between the subunits; the asymmetry of EPIC supports such an interpretation." @default.
• W2061057944 created "2016-06-24" @default.
• W2061057944 creator A5020266474 @default.
• W2061057944 creator A5039895393 @default.
• W2061057944 creator A5048918898 @default.
• W2061057944 creator A5075038023 @default.
• W2061057944 creator A5081693942 @default.
• W2061057944 date "2005-12-22" @default.
• W2061057944 modified "2023-10-07" @default.
• W2061057944 title "Fluoride Inhibition of Enolase: Crystal Structure and Thermodynamics^," @default.
• W2061057944 cites W1512618923 @default.
• W2061057944 cites W1539796472 @default.
• W2061057944 cites W1568517269 @default.
• W2061057944 cites W1964275052 @default.
• W2061057944 cites W1973024789 @default.
• W2061057944 cites W1981975492 @default.
• W2061057944 cites W1986191025 @default.
• W2061057944 cites W1995017064 @default.
• W2061057944 cites W2001641653 @default.
• W2061057944 cites W2016231457 @default.
• W2061057944 cites W2018513048 @default.
• W2061057944 cites W2028231353 @default.
• W2061057944 cites W2040456926 @default.
• W2061057944 cites W2040786841 @default.
• W2061057944 cites W2057664120 @default.
• W2061057944 cites W2073033441 @default.
• W2061057944 cites W2080092144 @default.
• W2061057944 cites W2080528351 @default.
• W2061057944 cites W2084377671 @default.
• W2061057944 cites W2089492488 @default.
• W2061057944 cites W2099657531 @default.
• W2061057944 cites W2100273026 @default.
• W2061057944 cites W2109574208 @default.
• W2061057944 cites W2140261762 @default.
• W2061057944 cites W3118440386 @default.
• W2061057944 cites W91085156 @default.
• W2061057944 doi "https://doi.org/10.1021/bi051558s" @default.
• W2061057944 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2566932" @default.
• W2061057944 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16411755" @default.
• W2061057944 hasPublicationYear "2005" @default.
• W2061057944 type Work @default.
• W2061057944 sameAs 2061057944 @default.
• W2061057944 citedByCount "75" @default.
• W2061057944 countsByYear W20610579442012 @default.
• W2061057944 countsByYear W20610579442013 @default.
• W2061057944 countsByYear W20610579442014 @default.
• W2061057944 countsByYear W20610579442015 @default.
• W2061057944 countsByYear W20610579442016 @default.
• W2061057944 countsByYear W20610579442017 @default.
• W2061057944 countsByYear W20610579442018 @default.
• W2061057944 countsByYear W20610579442019 @default.
• W2061057944 countsByYear W20610579442020 @default.
• W2061057944 countsByYear W20610579442021 @default.
• W2061057944 countsByYear W20610579442022 @default.
• W2061057944 countsByYear W20610579442023 @default.
• W2061057944 crossrefType "journal-article" @default.
• W2061057944 hasAuthorship W2061057944A5020266474 @default.
• W2061057944 hasAuthorship W2061057944A5039895393 @default.
• W2061057944 hasAuthorship W2061057944A5048918898 @default.
• W2061057944 hasAuthorship W2061057944A5075038023 @default.
• W2061057944 hasAuthorship W2061057944A5081693942 @default.
• W2061057944 hasBestOaLocation W20610579442 @default.
• W2061057944 hasConcept C115624301 @default.
• W2061057944 hasConcept C126322002 @default.
• W2061057944 hasConcept C147789679 @default.
• W2061057944 hasConcept C156911925 @default.
• W2061057944 hasConcept C161790260 @default.
• W2061057944 hasConcept C178790620 @default.
• W2061057944 hasConcept C179104552 @default.
• W2061057944 hasConcept C185592680 @default.
• W2061057944 hasConcept C188618488 @default.
• W2061057944 hasConcept C204232928 @default.
• W2061057944 hasConcept C2779546866 @default.
• W2061057944 hasConcept C2780828025 @default.
• W2061057944 hasConcept C41183919 @default.
• W2061057944 hasConcept C543218039 @default.
• W2061057944 hasConcept C55493867 @default.
• W2061057944 hasConcept C71240020 @default.
• W2061057944 hasConcept C71924100 @default.
• W2061057944 hasConcept C8010536 @default.
• W2061057944 hasConceptScore W2061057944C115624301 @default.
• W2061057944 hasConceptScore W2061057944C126322002 @default.
• W2061057944 hasConceptScore W2061057944C147789679 @default.
• W2061057944 hasConceptScore W2061057944C156911925 @default.
• W2061057944 hasConceptScore W2061057944C161790260 @default.
• W2061057944 hasConceptScore W2061057944C178790620 @default.
• W2061057944 hasConceptScore W2061057944C179104552 @default.
• W2061057944 hasConceptScore W2061057944C185592680 @default.
• W2061057944 hasConceptScore W2061057944C188618488 @default.
• W2061057944 hasConceptScore W2061057944C204232928 @default.
• W2061057944 hasConceptScore W2061057944C2779546866 @default.
• W2061057944 hasConceptScore W2061057944C2780828025 @default.
• W2061057944 hasConceptScore W2061057944C41183919 @default.
• W2061057944 hasConceptScore W2061057944C543218039 @default.
• W2061057944 hasConceptScore W2061057944C55493867 @default.
• W2061057944 hasConceptScore W2061057944C71240020 @default.
• W2061057944 hasConceptScore W2061057944C71924100 @default.
• W2061057944 hasConceptScore W2061057944C8010536 @default.

• next