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- W2061063721 abstract "Preovulatory follicles were isolated from immature rats 2 days after they were given a single injection of PMS gonadotrophin (PMSG). The follicles were either incubated intact or mechanically separated to obtain theca and granulosa cells. The samples were incubated for 2 h in a modified Krebs bicarbonate buffer containing albumin. The accumulation of progesterone, androstenedione, testosterone, and estradiol in the medium was determined by RIA. When the cells were isolated from rats killed before the LH surge, the intact follicles secreted predominantly estradiol in the hormone-free medium. The theca cells produced mainly androstenedione, and the granulosa cells produced mainly progesterone and estradiol. When LH was added to the incubation medium, the intact follicles increased the production of progesterone, androstenedione, and estradiol. In the theca cells, the in vitro addition of LH increased only the production of androstenedione while in the granulosa cells only progesterone was increased during t..." @default.
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- W2061063721 date "1978-09-01" @default.
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- W2061063721 title "Steroidogenesis in Isolated Cells of Preovulatory Rat Follicles*" @default.
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- W2061063721 doi "https://doi.org/10.1210/endo-103-3-771" @default.
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