FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061068182> ?p ?o ?g. }

• W2061068182 endingPage "e0119207" @default.
• W2061068182 startingPage "e0119207" @default.
• W2061068182 abstract "Nucleic acid aptamer selection is a powerful strategy for the development of regulatory agents for molecular intervention. Accordingly, aptamers have proven their diligence in the intervention with serine protease activities, which play important roles in physiology and pathophysiology. Nonetheless, there are only a few studies on the molecular basis underlying aptamer-protease interactions and the associated mechanisms of inhibition. In the present study, we use site-directed mutagenesis to delineate the binding sites of two 2´-fluoropyrimidine RNA aptamers (upanap-12 and upanap-126) with therapeutic potential, both binding to the serine protease urokinase-type plasminogen activator (uPA). We determine the subsequent impact of aptamer binding on the well-established molecular interactions (plasmin, PAI-1, uPAR, and LRP-1A) controlling uPA activities. One of the aptamers (upanap-126) binds to the area around the C-terminal α-helix in pro-uPA, while the other aptamer (upanap-12) binds to both the β-hairpin of the growth factor domain and the kringle domain of uPA. Based on the mapping studies, combined with data from small-angle X-ray scattering analysis, we construct a model for the upanap-12:pro-uPA complex. The results suggest and highlight that the size and shape of an aptamer as well as the domain organization of a multi-domain protein such as uPA, may provide the basis for extensive sterical interference with protein ligand interactions considered distant from the aptamer binding site." @default.
• W2061068182 created "2016-06-24" @default.
• W2061068182 creator A5011753158 @default.
• W2061068182 creator A5017464975 @default.
• W2061068182 creator A5026143955 @default.
• W2061068182 creator A5027036371 @default.
• W2061068182 creator A5028509658 @default.
• W2061068182 creator A5047355282 @default.
• W2061068182 creator A5068858393 @default.
• W2061068182 creator A5071117617 @default.
• W2061068182 creator A5074612956 @default.
• W2061068182 creator A5075943573 @default.
• W2061068182 date "2015-03-20" @default.
• W2061068182 modified "2023-10-17" @default.
• W2061068182 title "Protein-Binding RNA Aptamers Affect Molecular Interactions Distantly from Their Binding Sites" @default.
• W2061068182 cites W1480746595 @default.
• W2061068182 cites W1546092720 @default.
• W2061068182 cites W1591407067 @default.
• W2061068182 cites W1670322156 @default.
• W2061068182 cites W1753189859 @default.
• W2061068182 cites W1964858690 @default.
• W2061068182 cites W1992323997 @default.
• W2061068182 cites W1999839318 @default.
• W2061068182 cites W2002996779 @default.
• W2061068182 cites W2005113904 @default.
• W2061068182 cites W2010031911 @default.
• W2061068182 cites W2017871159 @default.
• W2061068182 cites W2020628782 @default.
• W2061068182 cites W2021375991 @default.
• W2061068182 cites W2037480447 @default.
• W2061068182 cites W2039092261 @default.
• W2061068182 cites W2050091361 @default.
• W2061068182 cites W2058622049 @default.
• W2061068182 cites W2062403128 @default.
• W2061068182 cites W2062695451 @default.
• W2061068182 cites W2063787921 @default.
• W2061068182 cites W2064283575 @default.
• W2061068182 cites W2066074720 @default.
• W2061068182 cites W2066088601 @default.
• W2061068182 cites W2069846304 @default.
• W2061068182 cites W2073864312 @default.
• W2061068182 cites W2077800633 @default.
• W2061068182 cites W2079321730 @default.
• W2061068182 cites W2083329111 @default.
• W2061068182 cites W2088480703 @default.
• W2061068182 cites W2104525153 @default.
• W2061068182 cites W2106434056 @default.
• W2061068182 cites W2109078461 @default.
• W2061068182 cites W2115997676 @default.
• W2061068182 cites W2124983865 @default.
• W2061068182 cites W2130630969 @default.
• W2061068182 cites W2137918942 @default.
• W2061068182 cites W2149885731 @default.
• W2061068182 cites W2152301430 @default.
• W2061068182 cites W2157467256 @default.
• W2061068182 cites W2331665234 @default.
• W2061068182 cites W2413744854 @default.
• W2061068182 doi "https://doi.org/10.1371/journal.pone.0119207" @default.
• W2061068182 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4368798" @default.
• W2061068182 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25793507" @default.
• W2061068182 hasPublicationYear "2015" @default.
• W2061068182 type Work @default.
• W2061068182 sameAs 2061068182 @default.
• W2061068182 citedByCount "19" @default.
• W2061068182 countsByYear W20610681822015 @default.
• W2061068182 countsByYear W20610681822016 @default.
• W2061068182 countsByYear W20610681822017 @default.
• W2061068182 countsByYear W20610681822018 @default.
• W2061068182 countsByYear W20610681822019 @default.
• W2061068182 countsByYear W20610681822020 @default.
• W2061068182 countsByYear W20610681822021 @default.
• W2061068182 countsByYear W20610681822022 @default.
• W2061068182 countsByYear W20610681822023 @default.
• W2061068182 crossrefType "journal-article" @default.
• W2061068182 hasAuthorship W2061068182A5011753158 @default.
• W2061068182 hasAuthorship W2061068182A5017464975 @default.
• W2061068182 hasAuthorship W2061068182A5026143955 @default.
• W2061068182 hasAuthorship W2061068182A5027036371 @default.
• W2061068182 hasAuthorship W2061068182A5028509658 @default.
• W2061068182 hasAuthorship W2061068182A5047355282 @default.
• W2061068182 hasAuthorship W2061068182A5068858393 @default.
• W2061068182 hasAuthorship W2061068182A5071117617 @default.
• W2061068182 hasAuthorship W2061068182A5074612956 @default.
• W2061068182 hasAuthorship W2061068182A5075943573 @default.
• W2061068182 hasBestOaLocation W20610681821 @default.
• W2061068182 hasConcept C104317684 @default.
• W2061068182 hasConcept C107824862 @default.
• W2061068182 hasConcept C11804247 @default.
• W2061068182 hasConcept C122682173 @default.
• W2061068182 hasConcept C128972844 @default.
• W2061068182 hasConcept C153911025 @default.
• W2061068182 hasConcept C161624437 @default.
• W2061068182 hasConcept C181199279 @default.
• W2061068182 hasConcept C185592680 @default.
• W2061068182 hasConcept C2776714187 @default.
• W2061068182 hasConcept C2777807008 @default.
• W2061068182 hasConcept C37600318 @default.
• W2061068182 hasConcept C40767141 @default.

• next