FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2061124657> ?p ?o ?g. }

• W2061124657 endingPage "79" @default.
• W2061124657 startingPage "69" @default.
• W2061124657 abstract "Background: Acylphosphatase (ACP) is a low molecular weight phosphomonohydrolase catalyzing with high specificity the hydrolysis of the carboxyl-phosphate bond present in acylphosphates. The enzyme is thought to regulate metabolic processes in which acylphosphates are involved, such as glycolysis and the production of ribonucleotides. Furthermore the enzyme is capable of hydrolyzing the phospho-aspartyl intermediate formed during the action of membrane pumps such as (Ca2++Mg2+) ATPase. Although the tertiary structure of a muscle ACP has been determined by NMR spectroscopy, little is known about the catalytic mechanism of ACP and further structures might provide an increased understanding.Results: The structure of ‘common type’ ACP from bovine testis has been determined by X-ray crystallography to a resolution of 1.8 Å. The structure has been refined to an R factor of 17.0 % using all data between 15 and 1.8 Å. The binding of a sulphate and a chloride ion in the active centre allows a detailed description of this site. The overall protein folds of common type and muscle ACP are similar but their loops have very different conformations. These differences, in part, are probably caused by the binding of the ions in the active site of the common type form. The phosphate-binding loop of ACP shows some remarkable similarities to that of low molecular weight protein tyrosine phosphatase.Conclusions: The active site of ACP has been located, enabling a reaction mechanism to be suggested in which the phosphate moiety bound to Arg23 acts as a base, abstracting a proton from a nucleophilic water molecule liganded to Asn41. The transition-state intermediate is stabilized by the phosphate-binding loop. We suggest the catalysis to be substrate assisted, which probably explains why this enzyme can only hydrolyze acylphosphates." @default.
• W2061124657 created "2016-06-24" @default.
• W2061124657 creator A5021055324 @default.
• W2061124657 creator A5028015945 @default.
• W2061124657 creator A5032609098 @default.
• W2061124657 creator A5047379462 @default.
• W2061124657 creator A5060431795 @default.
• W2061124657 date "1997-01-01" @default.
• W2061124657 modified "2023-10-15" @default.
• W2061124657 title "Crystal structure of common type acylphosphatase from bovine testis" @default.
• W2061124657 cites W1482051045 @default.
• W2061124657 cites W1521251288 @default.
• W2061124657 cites W1570569542 @default.
• W2061124657 cites W168286673 @default.
• W2061124657 cites W1880257727 @default.
• W2061124657 cites W1950746275 @default.
• W2061124657 cites W1964206110 @default.
• W2061124657 cites W1966041739 @default.
• W2061124657 cites W1975914851 @default.
• W2061124657 cites W1976846867 @default.
• W2061124657 cites W1986191025 @default.
• W2061124657 cites W1990866331 @default.
• W2061124657 cites W1993404146 @default.
• W2061124657 cites W1993772532 @default.
• W2061124657 cites W1996607909 @default.
• W2061124657 cites W1997196594 @default.
• W2061124657 cites W2000282440 @default.
• W2061124657 cites W2000840258 @default.
• W2061124657 cites W2001208346 @default.
• W2061124657 cites W2001641653 @default.
• W2061124657 cites W2004370807 @default.
• W2061124657 cites W2007829111 @default.
• W2061124657 cites W2013083986 @default.
• W2061124657 cites W2018243175 @default.
• W2061124657 cites W2023653296 @default.
• W2061124657 cites W2025881506 @default.
• W2061124657 cites W2026954909 @default.
• W2061124657 cites W2028231353 @default.
• W2061124657 cites W2031357465 @default.
• W2061124657 cites W2034890717 @default.
• W2061124657 cites W2034984854 @default.
• W2061124657 cites W2057984729 @default.
• W2061124657 cites W2060276346 @default.
• W2061124657 cites W2062489486 @default.
• W2061124657 cites W2071763386 @default.
• W2061124657 cites W2074612897 @default.
• W2061124657 cites W2076423964 @default.
• W2061124657 cites W2078248419 @default.
• W2061124657 cites W2080476827 @default.
• W2061124657 cites W2081867459 @default.
• W2061124657 cites W2083042788 @default.
• W2061124657 cites W2091114929 @default.
• W2061124657 cites W2115054713 @default.
• W2061124657 cites W2116275717 @default.
• W2061124657 cites W2123481095 @default.
• W2061124657 cites W2167938143 @default.
• W2061124657 cites W63904086 @default.
• W2061124657 doi "https://doi.org/10.1016/s0969-2126(97)00167-6" @default.
• W2061124657 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9016712" @default.
• W2061124657 hasPublicationYear "1997" @default.
• W2061124657 type Work @default.
• W2061124657 sameAs 2061124657 @default.
• W2061124657 citedByCount "84" @default.
• W2061124657 countsByYear W20611246572012 @default.
• W2061124657 countsByYear W20611246572013 @default.
• W2061124657 countsByYear W20611246572015 @default.
• W2061124657 countsByYear W20611246572017 @default.
• W2061124657 countsByYear W20611246572019 @default.
• W2061124657 countsByYear W20611246572020 @default.
• W2061124657 countsByYear W20611246572023 @default.
• W2061124657 crossrefType "journal-article" @default.
• W2061124657 hasAuthorship W2061124657A5021055324 @default.
• W2061124657 hasAuthorship W2061124657A5028015945 @default.
• W2061124657 hasAuthorship W2061124657A5032609098 @default.
• W2061124657 hasAuthorship W2061124657A5047379462 @default.
• W2061124657 hasAuthorship W2061124657A5060431795 @default.
• W2061124657 hasBestOaLocation W20611246571 @default.
• W2061124657 hasConcept C107824862 @default.
• W2061124657 hasConcept C181199279 @default.
• W2061124657 hasConcept C185592680 @default.
• W2061124657 hasConcept C23265538 @default.
• W2061124657 hasConcept C2776568683 @default.
• W2061124657 hasConcept C2780120296 @default.
• W2061124657 hasConcept C41183919 @default.
• W2061124657 hasConcept C47701112 @default.
• W2061124657 hasConcept C55493867 @default.
• W2061124657 hasConcept C66974803 @default.
• W2061124657 hasConcept C71240020 @default.
• W2061124657 hasConceptScore W2061124657C107824862 @default.
• W2061124657 hasConceptScore W2061124657C181199279 @default.
• W2061124657 hasConceptScore W2061124657C185592680 @default.
• W2061124657 hasConceptScore W2061124657C23265538 @default.
• W2061124657 hasConceptScore W2061124657C2776568683 @default.
• W2061124657 hasConceptScore W2061124657C2780120296 @default.
• W2061124657 hasConceptScore W2061124657C41183919 @default.
• W2061124657 hasConceptScore W2061124657C47701112 @default.
• W2061124657 hasConceptScore W2061124657C55493867 @default.
• W2061124657 hasConceptScore W2061124657C66974803 @default.

• next